FastQCFastQC Report
Fri 10 Feb 2017
ERR1630066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116025
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38520.34515355838802897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.27777155529670033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24490.2194395286843933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.16325799153244774No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGAT11540.10340270155238457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.026.9289951
AGCGTAC500.00703475818.4999985
CGAGCGT2750.018.1636376
CCGCGCA852.7230006E-517.4117649
TACGAGC3100.016.7096774
GCTACGA3100.016.7096772
ACCTAGA1005.877484E-616.651
GTATCAA13750.016.1454542
TTCGCGG2550.015.96078631
ATTCGCG1059.342533E-615.85714230
CAATTCG1059.342533E-615.85714228
CGGTCGA2700.015.75925920
TCTTGCG957.0593815E-515.5789487
CGCGCTT2850.015.57894712
GGCTACG3450.015.5507251
GTTCGCG1557.205017E-915.51612930
GTCGAAA2750.015.47272822
CCTACAC5750.015.4434793
CGCGGAA2650.015.35849133
ACGCTCG1701.4861143E-915.23529420
Produced by FastQC (version 0.11.5)