##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1116025
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.820479827960845	34.0	31.0	34.0	31.0	34.0
2	33.05261799690867	34.0	33.0	34.0	31.0	34.0
3	33.173570484532156	34.0	33.0	34.0	31.0	34.0
4	36.47159696243364	37.0	37.0	37.0	35.0	37.0
5	36.41135727246253	37.0	37.0	37.0	35.0	37.0
6	36.45246029434824	37.0	37.0	37.0	35.0	37.0
7	36.38128088528483	37.0	37.0	37.0	35.0	37.0
8	36.385180439506286	37.0	37.0	37.0	35.0	37.0
9	38.158092336641204	39.0	39.0	39.0	37.0	39.0
10	38.11752693712059	39.0	39.0	39.0	37.0	39.0
11	38.1515333437871	39.0	39.0	39.0	37.0	39.0
12	38.08272753746556	39.0	38.0	39.0	37.0	39.0
13	38.10033108577317	39.0	39.0	39.0	37.0	39.0
14	39.53832933850048	41.0	39.0	41.0	37.0	41.0
15	39.51441858381309	41.0	39.0	41.0	37.0	41.0
16	39.42360520597657	41.0	39.0	41.0	36.0	41.0
17	39.330045473891715	41.0	39.0	41.0	36.0	41.0
18	39.22710243946148	41.0	39.0	41.0	36.0	41.0
19	39.22481127214892	41.0	39.0	41.0	36.0	41.0
20	39.47829484106539	41.0	39.0	41.0	37.0	41.0
21	39.50202280414865	41.0	39.0	41.0	37.0	41.0
22	39.51468112273471	41.0	39.0	41.0	37.0	41.0
23	39.43739432360386	41.0	39.0	41.0	37.0	41.0
24	39.40399363813535	41.0	39.0	41.0	36.0	41.0
25	39.37295132277503	41.0	39.0	41.0	36.0	41.0
26	39.224399095002354	41.0	39.0	41.0	36.0	41.0
27	39.13714029703635	41.0	39.0	41.0	36.0	41.0
28	39.09731412826773	41.0	39.0	41.0	35.0	41.0
29	38.976068636455274	40.0	39.0	41.0	35.0	41.0
30	38.8761524159405	40.0	39.0	41.0	35.0	41.0
31	38.743113281512514	40.0	39.0	41.0	35.0	41.0
32	38.66526556304742	40.0	38.0	41.0	35.0	41.0
33	38.562944378486144	40.0	38.0	41.0	35.0	41.0
34	38.490816065948344	40.0	38.0	41.0	35.0	41.0
35	38.36094352725073	40.0	38.0	41.0	34.0	41.0
36	38.322047445173716	40.0	38.0	41.0	34.0	41.0
37	38.27298044398647	40.0	38.0	41.0	34.0	41.0
38	38.16290674492059	40.0	38.0	41.0	34.0	41.0
39	38.10406666517327	40.0	38.0	41.0	34.0	41.0
40	38.03127976523823	40.0	38.0	41.0	33.0	41.0
41	37.92650971080397	40.0	38.0	41.0	33.0	41.0
42	37.82028897202124	40.0	38.0	41.0	33.0	41.0
43	37.514069129275775	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	3.0
15	4.0
16	10.0
17	14.0
18	23.0
19	55.0
20	121.0
21	268.0
22	475.0
23	799.0
24	1311.0
25	1959.0
26	2770.0
27	3980.0
28	5438.0
29	7207.0
30	9285.0
31	12061.0
32	15583.0
33	20125.0
34	27265.0
35	38258.0
36	57550.0
37	101287.0
38	211745.0
39	598424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.12221948433055	17.86420555095092	11.816401962321631	26.1971730023969
2	20.314688291032905	20.03826079164893	33.71080396944513	25.93624694787303
3	19.749736789050424	21.50740350798593	29.7284559037656	29.014403799198046
4	14.953607670079075	15.546694742501288	33.546739544365046	35.952958043054586
5	15.740776416298917	35.04374901995923	33.50059362469479	15.714880939047065
6	35.7567258797966	33.81743240518806	14.934611679845883	15.491230035169462
7	30.656929728276694	29.43518290360879	20.499361573441455	19.40852579467306
8	27.650097444053674	32.935194104074725	18.942317600412178	20.472390851459423
9	26.725745391008264	14.196366568849264	19.127976523823392	39.949911516319084
10	17.407316144351608	26.11724647745346	32.70061154544029	23.774825832754644
11	36.34963374476378	20.999887995340604	21.253197733025694	21.39728052686992
12	21.660715485764207	24.391389081785803	29.644407607356467	24.303487825093523
13	30.941511166864544	19.4117515288636	23.523666584529916	26.123070719741943
14	22.637575323133444	20.31504670594297	25.58114737573083	31.466230595192762
15	25.682309984095337	26.966241795658703	22.109809368069712	25.24163885217625
16	24.514414999663988	26.2501288053583	23.871060236105823	25.36439595887189
17	24.686543760220424	25.80219977151049	25.306332743442127	24.20492372482695
18	24.138258551555744	25.058220021952916	25.7582043413006	25.045317085190742
19	25.3376940480724	25.032324544701062	25.92612172666383	23.70385968056271
20	25.03393741179633	24.810555319101272	25.806679957886246	24.348827311216148
21	24.786093501489663	24.523912994780584	25.95165878900562	24.738334714724132
22	25.100512981340025	24.898277368338523	25.58347707264622	24.41773257767523
23	24.607961291189714	25.11986738648328	25.41045227481463	24.861719047512377
24	25.08985013776573	25.02650030241258	25.51797674783271	24.36567281198898
25	25.13465200152326	24.209403911202706	25.788759212383233	24.867184874890796
26	25.4281042091351	24.56539952062006	25.878452543625812	24.128043726619026
27	24.568356443628055	25.04558589637329	25.69297282766963	24.693084832329024
28	24.469881947088997	25.147286127102888	25.4524764230192	24.930355502788917
29	24.28646311686566	24.959028695593737	26.16957505432226	24.584933133218343
30	24.31916847740866	25.178647431733157	25.625142805940726	24.877041284917453
31	24.598642503528147	24.43825183127618	25.97343249479178	24.98967317040389
32	24.45223001276853	24.841379001366455	26.20711901615107	24.49927196971394
33	24.10187943818463	24.733944132075894	26.640890661051497	24.523285768687977
34	24.888241750856835	23.95645258842768	25.829528908402587	25.325776752312894
35	23.950001120046593	24.09238144306803	26.521717703456467	25.43589973342891
36	24.54416343719899	23.683430030689276	26.494030151654307	25.27837638045743
37	24.129208575076724	23.38030062050581	27.12788692009588	25.362603884321587
38	23.648842991868463	23.546157120136197	27.916937344593535	24.888062543401805
39	23.141685894133197	23.234873770748862	28.44739141148272	25.176048923635225
40	22.888644967630654	23.06588114065545	28.812168186196548	25.23330570551735
41	22.69752021684102	23.260231625635626	28.634663201989202	25.40758495553415
42	22.49492618892946	23.734593759100377	28.50473779709236	25.265742254877804
43	22.816513966981024	23.3808382428709	28.49998879953406	25.302658990614006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	964.0
1	865.5
2	767.0
3	1245.0
4	1723.0
5	1723.0
6	1869.5
7	2016.0
8	1733.0
9	1450.0
10	1955.0
11	2460.0
12	2460.0
13	3865.0
14	5270.0
15	6396.0
16	7522.0
17	7323.5
18	7125.0
19	7125.0
20	8540.0
21	9955.0
22	9190.0
23	8425.0
24	9869.5
25	11314.0
26	11314.0
27	13648.0
28	15982.0
29	18742.5
30	21503.0
31	24553.0
32	27603.0
33	27603.0
34	31092.0
35	34581.0
36	37314.0
37	40047.0
38	43190.5
39	46334.0
40	46334.0
41	50475.0
42	54616.0
43	58421.0
44	62226.0
45	65217.0
46	68208.0
47	68208.0
48	73283.0
49	78358.0
50	81604.5
51	84851.0
52	88184.0
53	91517.0
54	91517.0
55	88787.0
56	86057.0
57	81191.5
58	76326.0
59	72757.5
60	69189.0
61	69189.0
62	65743.5
63	62298.0
64	53647.0
65	44996.0
66	39644.5
67	34293.0
68	34293.0
69	27747.0
70	21201.0
71	18072.5
72	14944.0
73	12085.0
74	9226.0
75	9226.0
76	7508.0
77	5790.0
78	4483.5
79	3177.0
80	2550.0
81	1923.0
82	1923.0
83	1438.0
84	953.0
85	715.0
86	477.0
87	340.5
88	204.0
89	204.0
90	147.5
91	91.0
92	64.0
93	37.0
94	25.5
95	14.0
96	14.0
97	11.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1116025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.82287067073464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.29228060529744	49.22960459850514
2	10.420440325196637	12.467613078126929
3	2.9514552236925797	5.296935724122761
4	1.3293064569665745	3.180917130275355
5	0.7562154495734513	2.261948951952202
6	0.4575778164736349	1.6424171122019646
7	0.30302212201539375	1.2689357250988929
8	0.22214188755064415	1.063133232759605
9	0.16902578705425778	0.9100447019069399
>10	0.963962036818327	10.473816195876397
>50	0.0715296468269368	2.968515465479816
>100	0.058065594224083916	6.409380061427495
>500	0.004072130435560236	1.6194420926160877
>1k	9.049178745689413E-4	1.2072959296504469
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3852	0.34515355838802897	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3100	0.27777155529670033	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2449	0.2194395286843933	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1822	0.16325799153244774	No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGAT	1154	0.10340270155238457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.688111825451939E-4	0.0
10	0.0	0.0	0.0	4.480186375753231E-4	0.0
11	0.0	0.0	0.0	8.960372751506462E-4	0.0
12	0.0	0.0	0.0	0.0029569230079971327	0.0
13	0.0	0.0	0.0	0.0030465267355121975	0.0
14	0.0	0.0	0.0	0.0030465267355121975	0.0
15	0.0	0.0	0.0	0.0030465267355121975	0.0
16	0.0	0.0	0.0	0.0030465267355121975	0.0
17	0.0	0.0	0.0	0.003136130463027262	0.0
18	0.0	0.0	0.0	0.003136130463027262	0.0
19	0.0	0.0	0.0	0.0032257341905423268	0.0
20	0.0	0.0	0.0	0.003942564010662844	0.0
21	0.0	0.0	0.0	0.004300978920723102	0.0
22	0.0	0.0	0.0	0.005017808740843619	0.0
23	0.0	0.0	0.0	0.008870769023991398	0.0
24	0.0	0.0	0.0	0.015949463497681504	0.0
25	0.0	0.0	0.0	0.028583589077305616	0.0
26	0.0	0.0	0.0	0.04462265630250219	0.0
27	0.0	0.0	0.0	0.13341995026993123	0.0
28	0.0	0.0	0.0	0.2839542124952398	0.0
29	0.0	0.0	0.0	0.44515131829484106	0.0
30	0.0	0.0	0.0	0.6236419435048498	0.0
31	8.960372751506463E-5	0.0	0.0	0.9443336842812661	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	845	0.0	26.928995	1
AGCGTAC	50	0.007034758	18.499998	5
CGAGCGT	275	0.0	18.163637	6
CCGCGCA	85	2.7230006E-5	17.411764	9
TACGAGC	310	0.0	16.709677	4
GCTACGA	310	0.0	16.709677	2
ACCTAGA	100	5.877484E-6	16.65	1
GTATCAA	1375	0.0	16.145454	2
TTCGCGG	255	0.0	15.960786	31
ATTCGCG	105	9.342533E-6	15.857142	30
CAATTCG	105	9.342533E-6	15.857142	28
CGGTCGA	270	0.0	15.759259	20
TCTTGCG	95	7.0593815E-5	15.578948	7
CGCGCTT	285	0.0	15.578947	12
GGCTACG	345	0.0	15.550725	1
GTTCGCG	155	7.205017E-9	15.516129	30
GTCGAAA	275	0.0	15.472728	22
CCTACAC	575	0.0	15.443479	3
CGCGGAA	265	0.0	15.358491	33
ACGCTCG	170	1.4861143E-9	15.235294	20
>>END_MODULE