FastQCFastQC Report
Fri 10 Feb 2017
ERR1630065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105424
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29730.2689465761553938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.2322185876188684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21210.19187207804426176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.1394939860180347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.027.2480621
CGAATTA2050.020.756115
CGAGCGT500.007034715718.56
TACGGAT700.0025924115.85714210
CCGAATT2750.015.47272714
GTATCAA11450.015.1877732
CAATACT1601.0959411E-815.031254
GTACACT1508.0986865E-814.84
GTAATAC1508.0986865E-814.83
GTGTAAC1252.9583116E-614.7999991
GTCTAGG1551.2097553E-714.3225811
CCCGAAT3100.014.32258113
AATGCGT1458.9082823E-714.03448335
TAATCTA2252.7284841E-1113.977777520
TAATACT2700.013.7037034
GTTCTAC2457.2759576E-1213.5918361
AATACTG3150.013.5079375
GTCTATA1102.4562262E-413.4545451
TAGGACA2408.0035534E-1113.1041664
GGCGTAG850.00940443213.0588241
Produced by FastQC (version 0.11.5)