FastQCFastQC Report
Fri 10 Feb 2017
ERR1630064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106397
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90550.4298809768528915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78400.3721995426313273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60960.28940413416844024No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.20527944162472694No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.10116801343716308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.027.8126411
CGTGCGA957.1540853E-921.42105310
CTATGCG500.00703678118.4999989
CGAATTA2000.017.57499915
TTAACGG752.068297E-417.26666635
GTATCAA37400.016.4719262
ATCTCGC4850.015.639173511
GCTCTAT1353.9764018E-715.0740751
AATCTCG5000.014.79999910
TAACGGC908.2780485E-414.38888836
AACGGCC908.2780485E-414.38888837
TTCCTCG5200.014.23076919
GTACGAC800.00630091813.8753
GTCTTAG2950.013.1694921
GATTAGC850.009408127513.0588241
CTTATAC19200.013.00781337
TCTCGCC5750.012.86956512
TATCAAC48500.012.8546393
TACTCCG1602.6978305E-612.718755
ATCAACG48900.012.598164
Produced by FastQC (version 0.11.5)