##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630064.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2106397
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.871946741283814	34.0	31.0	34.0	31.0	34.0
2	33.079654974821935	34.0	33.0	34.0	31.0	34.0
3	33.20183422213381	34.0	33.0	34.0	31.0	34.0
4	36.48819097254696	37.0	37.0	37.0	35.0	37.0
5	36.43873780678571	37.0	37.0	37.0	35.0	37.0
6	36.490944964315844	37.0	37.0	37.0	35.0	37.0
7	36.42180082861873	37.0	37.0	37.0	35.0	37.0
8	36.43295969373295	37.0	37.0	37.0	35.0	37.0
9	38.2061282844592	39.0	39.0	39.0	37.0	39.0
10	38.19099343571036	39.0	39.0	39.0	37.0	39.0
11	38.229156232182255	39.0	39.0	39.0	37.0	39.0
12	38.170463592570634	39.0	39.0	39.0	37.0	39.0
13	38.1821157170277	39.0	39.0	39.0	37.0	39.0
14	39.64107763161455	41.0	40.0	41.0	37.0	41.0
15	39.6392004925947	41.0	40.0	41.0	37.0	41.0
16	39.554198947301956	41.0	40.0	41.0	37.0	41.0
17	39.49383093500418	41.0	39.0	41.0	37.0	41.0
18	39.400366122815406	41.0	39.0	41.0	36.0	41.0
19	39.43764447062923	41.0	39.0	41.0	37.0	41.0
20	39.64664590768027	41.0	40.0	41.0	37.0	41.0
21	39.64600927555442	41.0	40.0	41.0	37.0	41.0
22	39.662264995629975	41.0	40.0	41.0	37.0	41.0
23	39.594408366514	41.0	40.0	41.0	37.0	41.0
24	39.56210059167384	41.0	40.0	41.0	37.0	41.0
25	39.53607653258147	41.0	40.0	41.0	37.0	41.0
26	39.39343485582253	41.0	39.0	41.0	36.0	41.0
27	39.3125298792203	41.0	39.0	41.0	36.0	41.0
28	39.27620339375721	41.0	39.0	41.0	36.0	41.0
29	39.17329734138436	41.0	39.0	41.0	36.0	41.0
30	39.08796679828161	41.0	39.0	41.0	35.0	41.0
31	38.953241008224	40.0	39.0	41.0	35.0	41.0
32	38.86093694588437	40.0	39.0	41.0	35.0	41.0
33	38.78834664120771	40.0	39.0	41.0	35.0	41.0
34	38.71432070972376	40.0	38.0	41.0	35.0	41.0
35	38.58747330156661	40.0	38.0	41.0	35.0	41.0
36	38.57484557754307	40.0	38.0	41.0	35.0	41.0
37	38.53281646337324	40.0	38.0	41.0	35.0	41.0
38	38.430653385852715	40.0	38.0	41.0	35.0	41.0
39	38.378243987244566	40.0	38.0	41.0	35.0	41.0
40	38.313171733533615	40.0	38.0	41.0	35.0	41.0
41	38.21114585712	40.0	38.0	41.0	34.0	41.0
42	38.11880713844541	40.0	38.0	41.0	34.0	41.0
43	37.82394296991498	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	8.0
16	16.0
17	46.0
18	105.0
19	164.0
20	236.0
21	445.0
22	744.0
23	1236.0
24	1999.0
25	3001.0
26	4403.0
27	6128.0
28	8596.0
29	11770.0
30	15506.0
31	19492.0
32	25580.0
33	33545.0
34	45978.0
35	64956.0
36	99420.0
37	177067.0
38	370945.0
39	1215006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.787850533398974	19.99067602166163	12.686165048658918	24.535308396280474
2	18.28743584424019	22.103952863586493	35.82230700100693	23.786304291166385
3	18.96755454930861	23.81288997278291	32.10985393541673	25.109701542491752
4	14.2033054547647	16.375972810443614	36.37424474113854	33.046476993653144
5	13.447844826972313	37.32515760324383	35.611900320784734	13.61509724899912
6	32.51917848344827	36.6711023610459	15.861777243321178	14.947941912184644
7	26.87461100637724	31.760869389768402	22.760002031905664	18.604517571948687
8	25.34085454926113	35.02079617470021	20.957967562619963	18.680381713418694
9	25.000083080255052	15.174442424671133	21.35907903400926	38.46639546106456
10	16.006146989385194	27.920282833672854	35.101265336021655	20.9723048409203
11	33.13924203272223	22.994905518760234	23.1745487673976	20.69130368111994
12	19.597302882600005	26.69207181742093	31.40220006010263	22.308425239876435
13	28.817597062661974	21.408547391588577	26.15902890100964	23.614826644739807
14	21.079739479309932	22.751931378557792	27.130735564093566	29.037593578038706
15	23.70445837133266	29.276959661450334	24.304582659394217	22.71399930782279
16	22.626219084056803	28.43723191781986	25.792858611173486	23.143690386949846
17	21.8830068595806	28.261054302678936	27.44387691399105	22.412061923749416
18	21.546887884857412	27.09522468936293	28.524869718291473	22.83301770748819
19	22.80534011394813	26.877981691010767	29.24942449120465	21.067253703836457
20	23.042047629198102	26.555345454821673	28.919477192571012	21.483129723409213
21	22.03981490668663	27.358660309523795	28.04333656001219	22.55818822377738
22	22.268404294157275	27.1256083254961	28.152622701228687	22.453364679117943
23	22.16405549381242	27.290819346970206	28.161547894342803	22.38357726487457
24	22.792142222002784	27.083071234909657	28.132873337742126	21.99191320534543
25	22.75686871942943	26.578987721687792	28.434620824089667	22.229522734793107
26	22.359080458242204	27.116303336930315	28.248948322657125	22.27566788217036
27	22.27467091910974	27.250893350113962	27.79993514992663	22.674500580849667
28	22.070293491682715	27.249374168307305	28.35989606897465	22.320436271035327
29	22.181858405609198	27.435853735074634	28.429778432080944	21.952509427235224
30	22.003496966621203	27.87185891358562	28.111937113469114	22.01270700632407
31	22.308899984191015	27.197342191429254	28.513191008152784	21.98056681622695
32	21.686842508795827	27.353343173200496	28.493963863412265	22.46585045459142
33	21.896679495840527	27.11089125174409	28.668242501294866	22.324186751120514
34	22.000221230850595	26.63491260194541	28.50972537465634	22.855140792547655
35	22.049262318546788	26.72544634273596	28.760437847186456	22.464853491530796
36	22.043422963477447	26.424505921723206	28.878981502537272	22.653089612262075
37	22.153278797871437	25.46680421591941	29.172848233262773	23.207068752946384
38	21.662820446478037	25.98465531426412	29.952853142118986	22.39967109713886
39	21.300638008884366	25.86606418448184	30.18035061766609	22.6529471889677
40	21.130964390853197	25.234559297226493	30.70067038644662	22.93380592547369
41	20.484742429845845	25.564743968017424	30.838820981989624	23.111692620147103
42	20.598870963071064	25.169614274991847	30.589010523657223	23.64250423827987
43	20.097066222559185	25.391462293195445	30.8616087090895	23.64986277515587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3058.0
1	2295.0
2	1532.0
3	2683.5
4	3835.0
5	3835.0
6	4184.0
7	4533.0
8	4025.0
9	3517.0
10	4941.0
11	6365.0
12	6365.0
13	9783.0
14	13201.0
15	17745.5
16	22290.0
17	23094.5
18	23899.0
19	23899.0
20	28558.5
21	33218.0
22	34640.5
23	36063.0
24	40582.5
25	45102.0
26	45102.0
27	50402.0
28	55702.0
29	66640.0
30	77578.0
31	81889.0
32	86200.0
33	86200.0
34	91100.5
35	96001.0
36	101740.5
37	107480.0
38	114528.0
39	121576.0
40	121576.0
41	128122.0
42	134668.0
43	131646.0
44	128624.0
45	129977.0
46	131330.0
47	131330.0
48	132731.5
49	134133.0
50	137337.0
51	140541.0
52	143287.5
53	146034.0
54	146034.0
55	134063.5
56	122093.0
57	113788.0
58	105483.0
59	95089.0
60	84695.0
61	84695.0
62	77958.0
63	71221.0
64	62775.5
65	54330.0
66	46953.0
67	39576.0
68	39576.0
69	33041.0
70	26506.0
71	22661.5
72	18817.0
73	15463.0
74	12109.0
75	12109.0
76	9403.5
77	6698.0
78	5478.5
79	4259.0
80	3167.5
81	2076.0
82	2076.0
83	1565.0
84	1054.0
85	802.0
86	550.0
87	398.5
88	247.0
89	247.0
90	180.5
91	114.0
92	79.5
93	45.0
94	32.5
95	20.0
96	20.0
97	15.5
98	11.0
99	12.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2106397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.89799037504862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.241875541715	44.051177111649196
2	11.816488840171472	12.974029812292057
3	3.5259093315116314	5.80696009633859
4	1.4358627993521575	3.1530392855490046
5	0.7736984518307538	2.1237245080897376
6	0.4535761739468094	1.4940252259009992
7	0.29328695674533845	1.1270605169973025
8	0.22250560349136358	0.9772088383090611
9	0.15043388558699544	0.7432666202732437
>10	0.9033305791227717	9.254562607651849
>50	0.08929136297956299	3.4245842081443647
>100	0.08430740355478347	9.255932686720396
>500	0.006900116012135055	2.61997072560487
>1k	0.0022709242571583715	1.8920465750666644
>5k	2.6202972197981214E-4	1.1024111814125466
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9055	0.4298809768528915	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7840	0.3721995426313273	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6096	0.28940413416844024	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4324	0.20527944162472694	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2131	0.10116801343716308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.494886291615493E-5	0.0
5	0.0	4.747443145807746E-5	0.0	9.494886291615493E-5	0.0
6	0.0	4.747443145807746E-5	0.0	9.494886291615493E-5	0.0
7	0.0	4.747443145807746E-5	0.0	9.494886291615493E-5	0.0
8	0.0	4.747443145807746E-5	0.0	9.494886291615493E-5	0.0
9	0.0	4.747443145807746E-5	0.0	1.424232943742324E-4	0.0
10	0.0	4.747443145807746E-5	0.0	3.797954516646197E-4	0.0
11	0.0	1.424232943742324E-4	0.0	4.272698831226972E-4	0.0
12	0.0	1.424232943742324E-4	0.0	6.17167608955007E-4	0.0
13	4.747443145807746E-5	1.424232943742324E-4	0.0	6.646420404130846E-4	0.0
14	4.747443145807746E-5	1.424232943742324E-4	0.0	8.070653347873169E-4	0.0
15	4.747443145807746E-5	1.424232943742324E-4	0.0	9.020141977034719E-4	0.0
16	4.747443145807746E-5	1.8989772583230985E-4	0.0	9.969630606196267E-4	0.0
17	4.747443145807746E-5	1.8989772583230985E-4	0.0	0.0010919119235357818	0.0
18	4.747443145807746E-5	1.8989772583230985E-4	0.0	0.0013767585122842466	0.0
19	9.494886291615493E-5	1.8989772583230985E-4	0.0	0.0015191818066584788	0.0
20	9.494886291615493E-5	1.8989772583230985E-4	0.0	0.0016616051010327113	0.0
21	1.424232943742324E-4	2.3737215729038733E-4	0.0	0.001946451689781176	0.0
22	1.424232943742324E-4	2.3737215729038733E-4	0.0	0.0028009914560265703	0.0
23	1.424232943742324E-4	2.848465887484648E-4	0.0	0.0050322897345562115	0.0
24	1.424232943742324E-4	2.848465887484648E-4	0.0	0.009684784017447804	0.0
25	1.424232943742324E-4	2.848465887484648E-4	0.0	0.01609383226428826	0.0
26	1.424232943742324E-4	2.848465887484648E-4	0.0	0.02829476114901417	0.0
27	1.8989772583230985E-4	2.848465887484648E-4	0.0	0.09300241122637376	0.0
28	1.8989772583230985E-4	2.848465887484648E-4	0.0	0.20451985072139772	0.0
29	1.8989772583230985E-4	3.323210202065423E-4	0.0	0.3312765827144646	0.0
30	1.8989772583230985E-4	3.323210202065423E-4	0.0	0.47080393676975424	0.0
31	1.8989772583230985E-4	3.323210202065423E-4	0.0	0.7380375114472723	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2215	0.0	27.812641	1
CGTGCGA	95	7.1540853E-9	21.421053	10
CTATGCG	50	0.007036781	18.499998	9
CGAATTA	200	0.0	17.574999	15
TTAACGG	75	2.068297E-4	17.266666	35
GTATCAA	3740	0.0	16.471926	2
ATCTCGC	485	0.0	15.6391735	11
GCTCTAT	135	3.9764018E-7	15.074075	1
AATCTCG	500	0.0	14.799999	10
TAACGGC	90	8.2780485E-4	14.388888	36
AACGGCC	90	8.2780485E-4	14.388888	37
TTCCTCG	520	0.0	14.230769	19
GTACGAC	80	0.006300918	13.875	3
GTCTTAG	295	0.0	13.169492	1
GATTAGC	85	0.0094081275	13.058824	1
CTTATAC	1920	0.0	13.007813	37
TCTCGCC	575	0.0	12.869565	12
TATCAAC	4850	0.0	12.854639	3
TACTCCG	160	2.6978305E-6	12.71875	5
ATCAACG	4890	0.0	12.59816	4
>>END_MODULE