FastQCFastQC Report
Fri 10 Feb 2017
ERR1630063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140669
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13810.9817372697609281No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.9660977187582196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9070.6447760345207544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.4535469790785461No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.17345683839367593No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.16137173080067393No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.13506884956884602No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.12795996274943305No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.1066333022911942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT251.2281247E-437.03
AACGAAC200.001836449537.025
ACCTTTA200.001836449537.030
TTAGATT401.5868864E-632.37528
CTTGCAA303.583579E-430.83333410
TAGATTT551.8313585E-830.27272829
CGAACAG250.005480159529.630
CCATACA250.005480159529.62
GAACCTT250.005480159529.628
TTTACGC453.9754686E-628.77777933
GTTAGAT453.9754686E-628.77777927
GAAAGTT453.9754686E-628.77777923
AAGTTAG453.9754686E-628.77777925
CATGAGT405.903702E-527.7500024
AGATTTA556.1963146E-726.9090930
TATGGGA358.8305917E-426.428572
TTACGCC509.017243E-625.90000234
GGTATCA4700.025.5851081
AAGACTT451.3151631E-424.6666685
TATACCA451.3151631E-424.6666685