##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630063.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	140669
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72275341404289	33.0	31.0	34.0	31.0	34.0
2	32.94212655240316	34.0	31.0	34.0	31.0	34.0
3	33.04464380922591	34.0	33.0	34.0	31.0	34.0
4	36.34284739352665	37.0	37.0	37.0	35.0	37.0
5	36.32659647825747	37.0	37.0	37.0	35.0	37.0
6	36.42126552403159	37.0	37.0	37.0	35.0	37.0
7	36.33954176115562	37.0	37.0	37.0	35.0	37.0
8	36.3734440423974	37.0	37.0	37.0	35.0	37.0
9	38.11966389183118	39.0	39.0	39.0	37.0	39.0
10	38.0915127000263	39.0	39.0	39.0	37.0	39.0
11	38.12727040072795	39.0	39.0	39.0	37.0	39.0
12	38.05253467359546	39.0	38.0	39.0	35.0	39.0
13	38.089913200491935	39.0	39.0	39.0	37.0	39.0
14	39.468511185833414	41.0	39.0	41.0	37.0	41.0
15	39.44743333641385	41.0	39.0	41.0	37.0	41.0
16	39.354399334608196	41.0	39.0	41.0	36.0	41.0
17	39.331842836730196	41.0	39.0	41.0	36.0	41.0
18	39.25869239135844	41.0	39.0	41.0	36.0	41.0
19	39.290831668669	41.0	39.0	41.0	36.0	41.0
20	39.490520299426315	41.0	39.0	41.0	37.0	41.0
21	39.47325992222878	41.0	39.0	41.0	37.0	41.0
22	39.47539969716142	41.0	39.0	41.0	36.0	41.0
23	39.39495553391294	41.0	39.0	41.0	36.0	41.0
24	39.35343963488757	41.0	39.0	41.0	36.0	41.0
25	39.319743511363555	41.0	39.0	41.0	36.0	41.0
26	39.117566770219454	41.0	39.0	41.0	35.0	41.0
27	39.00790508214319	41.0	39.0	41.0	35.0	41.0
28	38.9358067520207	40.0	39.0	41.0	35.0	41.0
29	38.80178290881431	40.0	38.0	41.0	35.0	41.0
30	38.6541668740092	40.0	38.0	41.0	35.0	41.0
31	38.46773631717009	40.0	38.0	41.0	35.0	41.0
32	38.322011246258946	40.0	38.0	41.0	35.0	41.0
33	38.216117268196975	40.0	38.0	41.0	35.0	41.0
34	38.12982959998294	40.0	38.0	41.0	34.0	41.0
35	37.93340394827574	40.0	37.0	41.0	34.0	41.0
36	37.87032679552709	40.0	37.0	41.0	34.0	41.0
37	37.77553689867704	40.0	37.0	41.0	33.0	41.0
38	37.62148021241354	40.0	37.0	41.0	33.0	41.0
39	37.529782681329934	40.0	37.0	41.0	33.0	41.0
40	37.38181831107067	40.0	37.0	41.0	33.0	41.0
41	37.209562874549476	40.0	36.0	41.0	33.0	41.0
42	37.033390441390786	40.0	36.0	41.0	31.0	41.0
43	36.68573744037421	40.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	1.0
15	1.0
16	1.0
17	2.0
18	6.0
19	4.0
20	18.0
21	24.0
22	78.0
23	85.0
24	167.0
25	297.0
26	412.0
27	599.0
28	839.0
29	1040.0
30	1409.0
31	1760.0
32	2213.0
33	2895.0
34	4047.0
35	5556.0
36	8196.0
37	15544.0
38	27222.0
39	68251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1838642486973	19.557969417568902	12.937463122649625	23.320703211084176
2	20.148007023580178	22.09513112341738	34.32526000753542	23.431601845467018
3	19.415791681180643	25.032523157198817	30.78858881487748	24.763096346743062
4	13.506174068202661	17.288101856130346	36.85531282656449	32.3504112491025
5	12.878459362048497	38.25718530735272	35.33401104721012	13.530344283388665
6	30.787877926195538	37.58184105950849	15.370124192252735	16.260156822043236
7	27.41257846433827	30.609444867028273	23.357669422545126	18.620307246088334
8	26.3647285471568	33.8326141509501	19.673133384043393	20.129523917849703
9	25.220197769231316	14.535540879653656	20.956998343629373	39.287263007485656
10	18.291876675031457	26.765669763771694	32.85229865855306	22.090154902643793
11	33.22196077316253	23.82259062053473	22.07025001954944	20.8851985867533
12	20.239711663550604	28.95094157205923	28.833644939538917	21.975701824851246
13	29.63197292935899	21.013158549502734	25.39934171708052	23.955526804057754
14	21.45035508889663	22.34038771868713	27.458786228664454	28.750470963751784
15	24.358600686718468	28.85283893395133	24.122585644313958	22.665974735016245
16	22.324748167684422	26.422310530394043	27.057845012049565	24.19509628987197
17	21.689924574710844	28.20948467679446	27.19433563898229	22.9062551095124
18	21.454620420988277	26.281554571369668	28.24147466748182	24.022350340160234
19	22.937534211517818	25.74270095045817	30.235517420327152	21.084247417696865
20	22.655311404787124	26.21544192394913	29.651877812453346	21.4773688588104
21	21.519311291044936	27.019457023224735	29.632683818040935	21.828547867689398
22	21.93375939261671	26.667567125663794	29.39880144168225	21.99987204003725
23	21.683526576573374	26.96400770603331	29.659697587954703	21.692768129438612
24	20.83046015824382	27.95285386261365	28.455452160746148	22.761233818396377
25	22.009113592902487	26.865194179243474	29.7784159978389	21.34727623001514
26	21.227136042767064	27.573239306457005	29.603537382081342	21.596087268694593
27	21.07145142142192	27.488643553305987	29.043357100711596	22.39654792456049
28	21.134009625432753	27.19291386161841	30.641434857715634	21.03164165523321
29	21.935892058662535	27.482245555168518	29.614200712310456	20.96766167385849
30	21.057233647783093	27.53485131763217	29.584343387668927	21.82357164691581
31	21.66433258216096	27.64930439542472	29.539557400706624	21.146805621707696
32	20.842545265836822	27.11969232737846	29.769885333655605	22.26787707312912
33	20.23331366541313	27.889584769920877	29.53102673652333	22.34607482814266
34	21.699166127576085	26.02847820059857	30.126040563308194	22.146315108517157
35	21.167421393483995	27.201444525801705	29.843817756577494	21.787316324136803
36	21.75319366740362	26.844578407467175	28.850706267905508	22.551521657223695
37	20.782119727871812	25.8948311283936	30.224143201416094	23.098905942318492
38	20.507716696642472	26.09387995933717	31.099247168885825	22.299156175134534
39	20.041373721288984	26.147907499164706	31.348058207565277	22.462660571981033
40	20.185684123723068	26.27444568455026	31.65231856343615	21.887551628290524
41	20.00227484378221	25.40147438312635	31.418436187077464	23.177814586013977
42	18.919591381185622	26.314255450738973	31.263462454414263	23.50269071366115
43	19.548727864703665	25.21166710504802	31.976483802401383	23.26312122784693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	251.5
2	201.0
3	310.0
4	419.0
5	419.0
6	469.0
7	519.0
8	461.5
9	404.0
10	551.0
11	698.0
12	698.0
13	1208.0
14	1718.0
15	2282.5
16	2847.0
17	2498.0
18	2149.0
19	2149.0
20	2379.5
21	2610.0
22	1856.5
23	1103.0
24	1071.5
25	1040.0
26	1040.0
27	1258.0
28	1476.0
29	2005.5
30	2535.0
31	3187.5
32	3840.0
33	3840.0
34	4625.0
35	5410.0
36	6155.5
37	6901.0
38	7745.0
39	8589.0
40	8589.0
41	9655.0
42	10721.0
43	11298.5
44	11876.0
45	12035.0
46	12194.0
47	12194.0
48	11981.5
49	11769.0
50	11484.0
51	11199.0
52	10210.0
53	9221.0
54	9221.0
55	8919.0
56	8617.0
57	7419.5
58	6222.0
59	5612.0
60	5002.0
61	5002.0
62	4335.5
63	3669.0
64	3140.5
65	2612.0
66	2219.5
67	1827.0
68	1827.0
69	1507.0
70	1187.0
71	1004.5
72	822.0
73	660.0
74	498.0
75	498.0
76	358.0
77	218.0
78	169.0
79	120.0
80	84.5
81	49.0
82	49.0
83	46.0
84	43.0
85	31.5
86	20.0
87	16.5
88	13.0
89	13.0
90	9.5
91	6.0
92	4.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	140669.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.380190375989024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40777021417898	40.28606160561318
2	10.581659195306878	10.873753278974045
3	3.5018540040954123	5.397777761980251
4	1.7986606895788366	3.6966211460947327
5	1.1068681166638994	2.8435547277651794
6	0.7761912668105595	2.3928513034143983
7	0.6198461453317837	2.2293469065679004
8	0.45243234268636895	1.8596847919584274
9	0.3583485527699375	1.6570815176051583
>10	2.2995185123692514	21.983521600352603
>50	0.07609718302064308	2.508015269888888
>100	0.015219436604128618	1.2255720876667922
>500	0.0027671702916597487	1.0983230135993005
>1k	0.0027671702916597487	1.9478349885191477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1381	0.9817372697609281	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1359	0.9660977187582196	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	907	0.6447760345207544	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	638	0.4535469790785461	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	244	0.17345683839367593	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	227	0.16137173080067393	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	190	0.13506884956884602	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.12795996274943305	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	150	0.1066333022911942	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0021326660458238843	0.0
23	0.0	0.0	0.0	0.002843554727765179	0.0
24	0.0	0.0	0.0	0.007819775501354242	0.0
25	0.0	0.0	0.0	0.014928662320767191	0.0
26	0.0	0.0	0.0	0.03554443409706474	0.0
27	0.0	0.0	0.0	0.12014018724807882	0.0
28	0.0	0.0	0.0	0.25307637077110096	0.0
29	0.0	0.0	0.0	0.43435298466613115	0.0
30	0.0	0.0	0.0	0.6426433684749305	0.0
31	0.0	0.0	0.0	0.9874243792164585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAT	25	1.2281247E-4	37.0	3
AACGAAC	20	0.0018364495	37.0	25
ACCTTTA	20	0.0018364495	37.0	30
TTAGATT	40	1.5868864E-6	32.375	28
CTTGCAA	30	3.583579E-4	30.833334	10
TAGATTT	55	1.8313585E-8	30.272728	29
CGAACAG	25	0.0054801595	29.6	30
CCATACA	25	0.0054801595	29.6	2
GAACCTT	25	0.0054801595	29.6	28
TTTACGC	45	3.9754686E-6	28.777779	33
GTTAGAT	45	3.9754686E-6	28.777779	27
GAAAGTT	45	3.9754686E-6	28.777779	23
AAGTTAG	45	3.9754686E-6	28.777779	25
CATGAGT	40	5.903702E-5	27.750002	4
AGATTTA	55	6.1963146E-7	26.90909	30
TATGGGA	35	8.8305917E-4	26.42857	2
TTACGCC	50	9.017243E-6	25.900002	34
GGTATCA	470	0.0	25.585108	1
AAGACTT	45	1.3151631E-4	24.666668	5
TATACCA	45	1.3151631E-4	24.666668	5
>>END_MODULE