FastQCFastQC Report
Fri 10 Feb 2017
ERR1630062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29171
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7752.6567481402763016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6752.313941928627747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4311.4774947722052723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3161.083267628809434No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.3496623358815262No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.3188097768331562No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.2948133420177574No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.2776730314353296No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.21939597545507522No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT560.19197147852319085TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT560.19197147852319085No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.17140310582427754No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.16797504370779198No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA470.16111891947482088No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA440.15083473312536422No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.14397860889239314No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA400.13712248465942203No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.13026636042645093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG370.12683829830996537No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.12341023619347982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGC650.001530573917.07692314
GGTATCA2900.016.5862061
TCTCTTA1153.3946335E-412.86956537
CCTGTCT1501.7951297E-512.33333337
GTCTCTT1502.490594E-411.136
GTATCAA4450.010.8089892
TACGGGG1200.00688402310.7916672
AACGCAG4150.010.2530127
GAGTACG2301.2637385E-59.65217411
AGTACGG2351.63105E-59.44680812
CGCAGAG4657.2759576E-129.15053759
TATCAAC5251.8189894E-128.8095243
TGTCTCT1900.00218531988.76315837
GTACGGG3054.199179E-68.4918031
CAGAGTA5351.4551915E-118.29906611
CAACGCA5351.4551915E-118.2990666
ACGCAGA5207.2759576E-118.1826938
GCAGAGT5207.2759576E-118.18269310
ATCAACG5452.1827873E-118.1467894
AGAGTAC5301.09139364E-108.02830212
Produced by FastQC (version 0.11.5)