##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630062.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29171
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.562373590209454	33.0	31.0	34.0	31.0	34.0
2	32.72280689726098	34.0	31.0	34.0	31.0	34.0
3	32.823249117274	34.0	31.0	34.0	31.0	34.0
4	36.14661821672209	37.0	35.0	37.0	35.0	37.0
5	36.175105412910085	37.0	35.0	37.0	35.0	37.0
6	36.30578314079051	37.0	37.0	37.0	35.0	37.0
7	36.180418909190635	37.0	37.0	37.0	35.0	37.0
8	36.2737993212437	37.0	37.0	37.0	35.0	37.0
9	37.95488670254705	39.0	38.0	39.0	35.0	39.0
10	37.96565081759282	39.0	38.0	39.0	35.0	39.0
11	37.96085153062973	39.0	38.0	39.0	35.0	39.0
12	37.941517260292756	39.0	38.0	39.0	35.0	39.0
13	37.976963422577214	39.0	38.0	39.0	35.0	39.0
14	39.18408693565527	40.0	39.0	41.0	36.0	41.0
15	39.18117308285626	40.0	39.0	41.0	36.0	41.0
16	39.0114154468479	40.0	38.0	41.0	36.0	41.0
17	39.14085907236639	40.0	39.0	41.0	36.0	41.0
18	39.122896026876006	40.0	39.0	41.0	36.0	41.0
19	39.15426279524185	40.0	39.0	41.0	36.0	41.0
20	39.34643995749203	41.0	39.0	41.0	36.0	41.0
21	39.30691440128895	41.0	39.0	41.0	36.0	41.0
22	39.22769188577697	41.0	39.0	41.0	35.0	41.0
23	39.10722978300367	41.0	39.0	41.0	35.0	41.0
24	39.01498063144904	41.0	39.0	41.0	35.0	41.0
25	38.95262418155017	41.0	39.0	41.0	35.0	41.0
26	38.643515820506664	40.0	38.0	41.0	35.0	41.0
27	38.41969764492133	40.0	38.0	41.0	35.0	41.0
28	38.236879092249154	40.0	37.0	41.0	34.0	41.0
29	37.97761475437935	40.0	37.0	41.0	34.0	41.0
30	37.65037194473964	40.0	36.0	41.0	33.0	41.0
31	37.334407459463165	39.0	35.0	41.0	33.0	41.0
32	37.0593054746152	39.0	35.0	41.0	33.0	41.0
33	36.798087141339	39.0	35.0	41.0	33.0	41.0
34	36.55298070001028	39.0	35.0	41.0	32.0	41.0
35	36.204243940900206	39.0	35.0	41.0	31.0	41.0
36	35.951390079188236	39.0	35.0	41.0	30.0	41.0
37	35.64584690274588	38.0	35.0	41.0	28.0	41.0
38	35.33372184703987	38.0	35.0	41.0	25.0	41.0
39	34.991944054026256	38.0	35.0	41.0	23.0	41.0
40	34.591032189503274	38.0	35.0	41.0	20.0	41.0
41	34.14120187857804	38.0	35.0	40.0	16.0	41.0
42	33.67145452675602	37.0	34.0	40.0	13.0	41.0
43	33.068424119845055	37.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	2.0
20	7.0
21	13.0
22	20.0
23	48.0
24	88.0
25	114.0
26	194.0
27	287.0
28	354.0
29	422.0
30	528.0
31	602.0
32	735.0
33	919.0
34	1177.0
35	1546.0
36	2218.0
37	4780.0
38	5446.0
39	9667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.46594220287271	17.84306331630729	14.48356244215145	19.20743203866854
2	20.38668540673957	24.87059065510267	32.47746049158411	22.265263446573652
3	23.194268280141237	25.206540742518253	31.438757670288986	20.160433307051523
4	14.30873127421069	16.807788557128656	32.240924205546605	36.642555963114056
5	13.67453978266086	38.60340749374379	31.7678516334716	15.954201090123753
6	32.326625758458746	33.44417400843303	14.003633745843475	20.22556648726475
7	25.74131843269	31.593020465530834	21.78190668814919	20.883754413629976
8	29.368208151931714	32.083233348188266	18.31270782626581	20.235850673614205
9	25.371087724109557	14.003633745843475	20.331836412875802	40.29344211717116
10	20.15700524493504	27.89071337972644	28.26094408830688	23.69133728703164
11	32.90939631826129	23.012580987967503	20.513523705049536	23.564498988721674
12	21.511089780946833	30.324637482431182	27.640464845222994	20.52380789139899
13	32.864831510746974	19.591374995714922	24.911727400500496	22.632066093037608
14	20.948887593843203	24.29124815741661	29.837852661890235	24.922011586849955
15	25.689897500942717	28.48719618799493	25.096842754790714	20.72606355627164
16	19.704501045558946	26.639470707209217	29.37849233828117	24.27753590895067
17	20.777484488018924	28.32264920640362	28.53861711974221	22.36124918583525
18	21.8504679304789	23.876452641321862	29.71787048781324	24.555208940386
19	22.950875869870764	25.329950978711736	32.40547118713791	19.31370196427959
20	22.59778547187275	26.13554557608584	31.10966370710637	20.15700524493504
21	21.637928079256795	26.612046210277327	32.083233348188266	19.666792362277604
22	20.71577936992218	28.01412361591992	32.05923691337287	19.210860100785027
23	20.647218127592474	27.61304034829111	31.682150080559463	20.05759144355696
24	20.335264474992286	27.434781118233865	31.98381954681019	20.246134859963664
25	19.728497480374344	27.22909739124473	32.13465427993555	20.907750848445374
26	19.98560213911076	28.360357889684963	32.823694765349146	18.83034520585513
27	19.735353604607315	27.842720510095642	32.947105001542624	19.47482088375441
28	19.039456994960748	28.86085495869185	33.241918343560386	18.857769702787014
29	19.10801823729046	28.740872784614858	32.751705460902954	19.399403517191733
30	18.54581605018683	29.844708786123203	32.62829522470947	18.981179938980492
31	18.49782318055603	29.872133283055092	32.686572280689724	18.943471255699155
32	18.5766686092352	29.666449556065956	32.62829522470947	19.128586609989373
33	18.487538994206577	30.02296801618045	32.357478317507116	19.13201467210586
34	18.60409310616708	29.652737307600013	31.75071132288917	19.99245826334373
35	17.932192931335916	29.9406945253848	32.50488498851599	19.622227554763292
36	18.32299201261527	29.083678996263412	32.40547118713791	20.18785780398341
37	17.579102533337903	29.200233108223923	32.9299646909602	20.290699667477973
38	17.630523465085187	28.744300846731342	33.12193616948339	20.503239518700077
39	17.44198004867848	29.035686126632616	33.44760207054952	20.074731754139385
40	17.232868259572864	29.3716362140482	33.135648417949334	20.259847108429604
41	16.626101264954922	29.05625449933153	33.44074594631655	20.876898289397
42	16.499262966644956	29.23794179150526	33.19735353604607	21.065441705803707
43	16.48212265606253	28.53861711974221	32.93339275307668	22.045867471118576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	61.0
1	50.5
2	40.0
3	100.0
4	160.0
5	160.0
6	210.0
7	260.0
8	244.5
9	229.0
10	284.5
11	340.0
12	340.0
13	657.0
14	974.0
15	1373.0
16	1772.0
17	1506.0
18	1240.0
19	1240.0
20	1307.0
21	1374.0
22	944.5
23	515.0
24	470.0
25	425.0
26	425.0
27	456.0
28	487.0
29	532.5
30	578.0
31	607.0
32	636.0
33	636.0
34	666.5
35	697.0
36	740.0
37	783.0
38	834.5
39	886.0
40	886.0
41	944.5
42	1003.0
43	1046.5
44	1090.0
45	1224.5
46	1359.0
47	1359.0
48	1516.5
49	1674.0
50	1733.0
51	1792.0
52	1743.0
53	1694.0
54	1694.0
55	1697.5
56	1701.0
57	1643.5
58	1586.0
59	1552.5
60	1519.0
61	1519.0
62	1392.5
63	1266.0
64	1123.0
65	980.0
66	847.5
67	715.0
68	715.0
69	612.5
70	510.0
71	429.5
72	349.0
73	286.0
74	223.0
75	223.0
76	173.5
77	124.0
78	98.0
79	72.0
80	52.5
81	33.0
82	33.0
83	23.0
84	13.0
85	8.0
86	3.0
87	3.5
88	4.0
89	4.0
90	3.0
91	2.0
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	29171.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.59905385485585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.40253026799074	69.09944808199924
2	5.917562236430418	8.947242124027287
3	1.251530404026663	2.8384354324500363
4	0.3854350881966172	1.1655411196050873
5	0.20405387022173854	0.7713139762092489
6	0.14510497437990297	0.6581879263652257
7	0.10429420033555524	0.5519180007541736
8	0.07708701763932345	0.4662164478420349
9	0.03627624359497574	0.24682047238695964
>10	0.42624586224096495	5.879126529772719
>50	0.027207182696231805	1.4946350827877002
>100	0.013603591348115902	2.9104247368962324
>500	0.009069060898743935	4.970690068904048
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	2.6567481402763016	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	675	2.313941928627747	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	431	1.4774947722052723	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	1.083267628809434	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102	0.3496623358815262	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93	0.3188097768331562	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.2948133420177574	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.2776730314353296	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64	0.21939597545507522	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	56	0.19197147852319085	TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	56	0.19197147852319085	No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50	0.17140310582427754	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49	0.16797504370779198	No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA	47	0.16111891947482088	No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA	44	0.15083473312536422	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42	0.14397860889239314	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	40	0.13712248465942203	No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.13026636042645093	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	37	0.12683829830996537	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.12341023619347982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0068561242329711015	0.0
11	0.0	0.0	0.0	0.0068561242329711015	0.0
12	0.0	0.0	0.0	0.0068561242329711015	0.0
13	0.0	0.0	0.0	0.0068561242329711015	0.0
14	0.0	0.0	0.0	0.0068561242329711015	0.0
15	0.0	0.0	0.0	0.0068561242329711015	0.0
16	0.0	0.0	0.0	0.0068561242329711015	0.0
17	0.0	0.0	0.0	0.010284186349456652	0.0
18	0.0	0.0	0.0	0.010284186349456652	0.0
19	0.0	0.0	0.0	0.010284186349456652	0.0
20	0.0	0.0	0.0	0.010284186349456652	0.0
21	0.0	0.0	0.0	0.010284186349456652	0.0
22	0.0	0.0	0.0	0.017140310582427754	0.0
23	0.0	0.0	0.0	0.020568372698913304	0.0
24	0.0	0.0	0.0	0.030852559048369955	0.0
25	0.0	0.0	0.0	0.04113674539782661	0.0
26	0.0	0.0	0.0	0.07884542867916766	0.0
27	0.0	0.0	0.0	0.1816872921737342	0.0
28	0.0	0.0	0.0	0.4387919509101505	0.0
29	0.0	0.0	0.0	0.8295910321895033	0.0
30	0.0	0.0	0.0	1.206677865002914	0.0
31	0.0	0.0	0.0	1.7345994309416886	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGC	65	0.0015305739	17.076923	14
GGTATCA	290	0.0	16.586206	1
TCTCTTA	115	3.3946335E-4	12.869565	37
CCTGTCT	150	1.7951297E-5	12.333333	37
GTCTCTT	150	2.490594E-4	11.1	36
GTATCAA	445	0.0	10.808989	2
TACGGGG	120	0.006884023	10.791667	2
AACGCAG	415	0.0	10.253012	7
GAGTACG	230	1.2637385E-5	9.652174	11
AGTACGG	235	1.63105E-5	9.446808	12
CGCAGAG	465	7.2759576E-12	9.1505375	9
TATCAAC	525	1.8189894E-12	8.809524	3
TGTCTCT	190	0.0021853198	8.763158	37
GTACGGG	305	4.199179E-6	8.491803	1
CAGAGTA	535	1.4551915E-11	8.299066	11
CAACGCA	535	1.4551915E-11	8.299066	6
ACGCAGA	520	7.2759576E-11	8.182693	8
GCAGAGT	520	7.2759576E-11	8.182693	10
ATCAACG	545	2.1827873E-11	8.146789	4
AGAGTAC	530	1.09139364E-10	8.028302	12
>>END_MODULE