FastQCFastQC Report
Fri 10 Feb 2017
ERR1630061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476586
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48331.0140876987574121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43790.9188268224412802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30230.6343031478054327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.5134435338008251No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18090.3795747252332213No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15080.3164171838870634No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.2872514089797015No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.24801399957195555No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.17457499800665569No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.14645835169308374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCT303.5963446E-430.8333344
CGCGTGC250.005493154329.68
GGTATCA11700.028.303421
CGTGCGA400.001929537723.12510
CGAGGTT450.003822571820.55555522
ATAGTGC450.003822571820.5555556
TAGAAAG555.137025E-420.1818185
TTGAGTG555.137025E-420.18181813
AGCACCC609.226003E-418.55
CCGTGCG609.226003E-418.59
GTTAATA500.007028986218.52
CTTGAGT609.226003E-418.512
GTAAGCA500.007028986218.530
TCTATAC609.226003E-418.53
GCGAGGT500.007028986218.521
CCGCCGT752.064068E-417.26666622
GGGGTAA650.001578100317.07692113
GTAAGGC650.001578100317.07692116
GTATCAA19650.016.9465662
TCTAATA1757.2759576E-1216.9142862
Produced by FastQC (version 0.11.5)