##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476586
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75744356737294	34.0	31.0	34.0	31.0	34.0
2	32.96697762838187	34.0	31.0	34.0	31.0	34.0
3	33.07679831132261	34.0	33.0	34.0	31.0	34.0
4	36.37308061923766	37.0	37.0	37.0	35.0	37.0
5	36.35796267620115	37.0	37.0	37.0	35.0	37.0
6	36.43960166685551	37.0	37.0	37.0	35.0	37.0
7	36.364960363921725	37.0	37.0	37.0	35.0	37.0
8	36.39481856370099	37.0	37.0	37.0	35.0	37.0
9	38.15402256885431	39.0	39.0	39.0	37.0	39.0
10	38.13548027008766	39.0	39.0	39.0	37.0	39.0
11	38.174230883827896	39.0	39.0	39.0	37.0	39.0
12	38.10338952466082	39.0	39.0	39.0	37.0	39.0
13	38.133505810074155	39.0	39.0	39.0	37.0	39.0
14	39.52195406495365	41.0	39.0	41.0	37.0	41.0
15	39.50716764655277	41.0	39.0	41.0	37.0	41.0
16	39.40413062909947	41.0	39.0	41.0	36.0	41.0
17	39.370971870764144	41.0	39.0	41.0	37.0	41.0
18	39.27219431540163	41.0	39.0	41.0	36.0	41.0
19	39.311704498243756	41.0	39.0	41.0	36.0	41.0
20	39.49300231227942	41.0	39.0	41.0	37.0	41.0
21	39.48794761071454	41.0	39.0	41.0	37.0	41.0
22	39.48276281720403	41.0	39.0	41.0	36.0	41.0
23	39.402065944026894	41.0	39.0	41.0	36.0	41.0
24	39.35960770983621	41.0	39.0	41.0	36.0	41.0
25	39.31649062288863	41.0	39.0	41.0	36.0	41.0
26	39.13347643447353	41.0	39.0	41.0	35.0	41.0
27	39.031585065444645	41.0	39.0	41.0	35.0	41.0
28	38.964235206237696	40.0	39.0	41.0	35.0	41.0
29	38.826323056069626	40.0	38.0	41.0	35.0	41.0
30	38.68880537825282	40.0	38.0	41.0	35.0	41.0
31	38.51931235915448	40.0	38.0	41.0	35.0	41.0
32	38.39145925394367	40.0	38.0	41.0	35.0	41.0
33	38.27375751700637	40.0	38.0	41.0	35.0	41.0
34	38.189357219893154	40.0	38.0	41.0	35.0	41.0
35	38.003269084698246	40.0	37.0	41.0	34.0	41.0
36	37.943886308032546	40.0	37.0	41.0	34.0	41.0
37	37.86182137116911	40.0	37.0	41.0	34.0	41.0
38	37.726357887138946	40.0	37.0	41.0	33.0	41.0
39	37.65504651836185	40.0	37.0	41.0	33.0	41.0
40	37.53153260901495	40.0	37.0	41.0	33.0	41.0
41	37.36886522054781	40.0	36.0	41.0	33.0	41.0
42	37.22477370296232	40.0	36.0	41.0	33.0	41.0
43	36.89384707062314	40.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	3.0
16	6.0
17	6.0
18	10.0
19	16.0
20	60.0
21	129.0
22	226.0
23	381.0
24	569.0
25	935.0
26	1321.0
27	1838.0
28	2485.0
29	3343.0
30	4288.0
31	5392.0
32	6999.0
33	9008.0
34	12756.0
35	18289.0
36	30140.0
37	52329.0
38	91595.0
39	234457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.280331356775065	20.246922905834413	13.286164511756535	23.186581225633986
2	19.25297847607777	22.607042590424392	35.1764424469036	22.963536486594236
3	19.00013848497438	25.385344932499066	31.517501563201606	24.09701501932495
4	13.233917907785795	17.59157843495193	37.89263637622591	31.28186728103637
5	12.70368831648433	38.253746438208424	35.743811190425234	13.298754054882014
6	29.762729077228457	38.267385109927694	15.907517216200223	16.06236859664363
7	26.71815789804988	31.246406734566268	23.904604835223864	18.13083053215999
8	25.639863529352517	34.516750387128454	20.625448502473844	19.217937581045184
9	24.887638327605092	14.75305611159371	21.78095873567415	38.57834682512705
10	17.516670653355323	27.134242298346994	33.79746782322603	21.551619225071654
11	32.25965513044865	24.110863516762976	22.908352322560884	20.72112903022749
12	19.743341180815214	28.891113041507722	30.00654656242525	21.35899921525181
13	29.305309010336018	21.39299098169061	26.421883983163585	22.87981602480979
14	20.87556075923338	22.949897814874966	28.17078134901151	28.00376007688014
15	24.003642574477638	29.1789939276437	24.767618016475517	22.049745481403146
16	21.514480072851487	27.26790128119584	27.768335620433664	23.449283025519005
17	21.159454956712953	28.418165871427192	27.981518550691796	22.440860621168056
18	21.118538941555144	26.579043446513328	29.147310244111242	23.15510736782029
19	23.00508197890832	25.876127288674027	30.90879715308465	20.209993579333005
20	22.28957627794354	26.536029174167936	30.186577029119615	20.98781751876891
21	21.26877415618587	27.05807556243784	30.257707947778574	21.415442333597714
22	21.724725443047006	26.930291699714214	30.013680636863022	21.331302220375754
23	21.367182418283374	27.272097795571	30.325271829218654	21.035447956926976
24	20.7960368118241	27.737071588338726	29.388399994964182	22.078491604872994
25	21.61498659213657	26.788869165271322	30.423470265597395	21.17267397699471
26	20.777572148573395	27.80547477265383	30.32317357203107	21.0937795067417
27	20.880386750764814	27.856462422312028	29.65949482359952	21.60365600332364
28	20.938298649142023	27.465347282547114	30.973003823024598	20.623350245286264
29	21.50377896119483	27.38036786645013	30.3999697850965	20.715883387258543
30	20.82562223816898	27.85415433940569	30.10915133889791	21.211072083527423
31	21.226809012434273	27.693847490274575	30.244908578934336	20.834434918356813
32	20.414783480840814	27.519482317986682	30.438997368785486	21.62673683238702
33	20.172224949956565	27.9265442123772	30.450747609035933	21.450483228630297
34	21.285979865124027	26.55008749732472	30.607277595229405	21.55665504232185
35	20.94186568636091	27.54046488986248	30.336182766594067	21.181486657182543
36	21.174982059901044	27.48087438573521	29.465615859467125	21.87852769489662
37	20.390233871746126	26.488608561728626	30.88424754398996	22.236910022535284
38	20.355402802432298	26.867763635524334	31.103725245810832	21.673108316232536
39	19.58135572593404	26.980440046497378	31.345024822382527	22.093179405186053
40	20.247972034428205	26.37215528781794	31.89665663699731	21.48321604075655
41	19.749845778096713	26.225696936124855	31.70172854427113	22.3227287415073
42	18.770589148653126	26.938474902745778	31.376079028758713	22.91485691984238
43	19.333341726362082	25.776250246545217	32.07710675512919	22.813301271963507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3599.0
1	2434.5
2	1270.0
3	1771.0
4	2272.0
5	2272.0
6	2320.5
7	2369.0
8	1997.0
9	1625.0
10	2173.0
11	2721.0
12	2721.0
13	4389.5
14	6058.0
15	7524.0
16	8990.0
17	8098.0
18	7206.0
19	7206.0
20	8189.0
21	9172.0
22	7022.5
23	4873.0
24	5118.5
25	5364.0
26	5364.0
27	6203.5
28	7043.0
29	8952.0
30	10861.0
31	12806.0
32	14751.0
33	14751.0
34	17536.5
35	20322.0
36	22565.5
37	24809.0
38	27166.5
39	29524.0
40	29524.0
41	32241.5
42	34959.0
43	37087.0
44	39215.0
45	38740.5
46	38266.0
47	38266.0
48	37235.5
49	36205.0
50	36188.5
51	36172.0
52	33169.0
53	30166.0
54	30166.0
55	28379.5
56	26593.0
57	22957.5
58	19322.0
59	17147.5
60	14973.0
61	14973.0
62	13133.5
63	11294.0
64	9822.0
65	8350.0
66	6839.5
67	5329.0
68	5329.0
69	4593.5
70	3858.0
71	3413.0
72	2968.0
73	2640.0
74	2312.0
75	2312.0
76	2013.0
77	1714.0
78	1414.0
79	1114.0
80	814.0
81	514.0
82	514.0
83	359.5
84	205.0
85	153.0
86	101.0
87	79.0
88	57.0
89	57.0
90	47.5
91	38.0
92	28.5
93	19.0
94	14.0
95	9.0
96	9.0
97	4.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	476586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.88199294438598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84843597313473	38.23302247885893
2	10.600316548827744	10.15128564398456
3	3.1855608228780876	4.575930025348828
4	1.4668528843359299	2.8094335783290085
5	0.8810461892027931	2.109312370754315
6	0.5757194856587692	1.6539957810155226
7	0.43869996644426607	1.4704080088590699
8	0.3126392793301915	1.1975833413620918
9	0.27956467699141635	1.2047502502082288
>10	2.129314425535093	20.504256925167507
>50	0.21936419039678004	7.289706857901021
>100	0.05805395745823104	4.115074957638089
>500	8.863199611943669E-4	0.32465656088208505
>1k	0.0035452798447774677	4.360583219690751
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4833	1.0140876987574121	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4379	0.9188268224412802	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3023	0.6343031478054327	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2447	0.5134435338008251	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1809	0.3795747252332213	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1508	0.3164171838870634	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1369	0.2872514089797015	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1182	0.24801399957195555	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	832	0.17457499800665569	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.14645835169308374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.0982571875799962E-4	0.0
4	0.0	0.0	0.0	2.0982571875799962E-4	0.0
5	0.0	0.0	0.0	2.0982571875799962E-4	0.0
6	0.0	0.0	0.0	2.0982571875799962E-4	0.0
7	0.0	0.0	0.0	2.0982571875799962E-4	0.0
8	0.0	0.0	0.0	2.0982571875799962E-4	0.0
9	0.0	0.0	0.0	4.1965143751599923E-4	0.0
10	0.0	0.0	0.0	6.294771562739988E-4	0.0
11	0.0	0.0	0.0	6.294771562739988E-4	0.0
12	0.0	0.0	0.0	6.294771562739988E-4	0.0
13	0.0	0.0	0.0	6.294771562739988E-4	0.0
14	0.0	0.0	0.0	8.393028750319985E-4	0.0
15	0.0	0.0	0.0	8.393028750319985E-4	0.0
16	0.0	0.0	0.0	8.393028750319985E-4	0.0
17	0.0	0.0	0.0	8.393028750319985E-4	0.0
18	0.0	0.0	0.0	8.393028750319985E-4	0.0
19	0.0	0.0	0.0	8.393028750319985E-4	0.0
20	0.0	0.0	0.0	0.0012589543125479975	0.0
21	0.0	0.0	0.0	0.0023080829063379955	0.0
22	0.0	0.0	0.0	0.0035670372188859934	0.0
23	0.0	0.0	0.0	0.006504597281497988	0.0
24	0.0	0.0	0.0	0.008812680187835983	0.0
25	0.0	0.0	0.0	0.015317277469333972	0.0
26	0.0	0.0	0.0	0.02643804056350795	0.0
27	0.0	0.0	0.0	0.09316261912855182	0.0
28	0.0	0.0	0.0	0.2171696189145296	0.0
29	0.0	0.0	0.0	0.3684539621390473	0.0
30	0.0	0.0	0.0	0.534845757114141	0.0
31	0.0	0.0	0.0	0.8437092151259165	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCT	30	3.5963446E-4	30.833334	4
CGCGTGC	25	0.0054931543	29.6	8
GGTATCA	1170	0.0	28.30342	1
CGTGCGA	40	0.0019295377	23.125	10
CGAGGTT	45	0.0038225718	20.555555	22
ATAGTGC	45	0.0038225718	20.555555	6
TAGAAAG	55	5.137025E-4	20.181818	5
TTGAGTG	55	5.137025E-4	20.181818	13
AGCACCC	60	9.226003E-4	18.5	5
CCGTGCG	60	9.226003E-4	18.5	9
GTTAATA	50	0.0070289862	18.5	2
CTTGAGT	60	9.226003E-4	18.5	12
GTAAGCA	50	0.0070289862	18.5	30
TCTATAC	60	9.226003E-4	18.5	3
GCGAGGT	50	0.0070289862	18.5	21
CCGCCGT	75	2.064068E-4	17.266666	22
GGGGTAA	65	0.0015781003	17.076921	13
GTAAGGC	65	0.0015781003	17.076921	16
GTATCAA	1965	0.0	16.946566	2
TCTAATA	175	7.2759576E-12	16.914286	2
>>END_MODULE