FastQCFastQC Report
Fri 10 Feb 2017
ERR1630060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198751
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5370.2701873198122273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.2445270715619041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4220.21232597571836115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.14238922068316637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTGC250.005485547729.624
GGTATCA1350.028.7777791
GCATTGC400.001925602423.12500230
TAACACT502.6912216E-422.24
CTGATAT502.6912216E-422.225
CTAGCAC502.6912216E-422.23
CTACTTA705.066975E-621.14285912
TTTAGCA450.003814851220.55555532
AGCAATA450.003814851220.55555535
ATTGCCC450.003814851220.55555532
TTGCAGT555.1225524E-420.1818184
ACTACTT656.8654896E-519.92307711
TAGTCAA656.8654896E-519.92307726
GTCAATA759.2027E-619.73333428
GATTAAT851.2347391E-619.5882341
GTATCAA2050.018.9512212
TACTTAA801.6057353E-518.513
TTAGCAA500.00701492818.533
GAATATA500.00701492818.51
GCTGATA500.00701492818.524
Produced by FastQC (version 0.11.5)