FastQCFastQC Report
Fri 10 Feb 2017
ERR1630059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189404
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36310.305278946430313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.276440973798642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27330.22977894811182742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20950.17613863750248024No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC20950.17613863750248024No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT19050.1601642503304176No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA15840.13317594358182755No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG15670.1317466563085377No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA13330.11207293737031321No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.10551503105757169No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.10030233629616178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9600.028.7135411
CTAGTAC400.001931226923.1253
CGTGCGA806.960545E-720.812510
CGAATTA1052.2595486E-819.3809515
AAGCTCG500.007035023518.49999818
GTATCAA15500.017.7838732
CGAGAAT1258.578354E-917.7614
GTAGCAC650.00157997817.0769233
GTCTAAT1005.878137E-616.651
CGACCAA1251.657736E-716.27999912
AAACCGT700.002592564815.8571436
CGGTCGT855.364344E-415.23529410
AGTCCGT1353.9721817E-715.0740756
CCGTGCG1252.95877E-614.7999999
ACCATCG1405.994825E-714.53571530
ATAGGAC1551.21001E-714.322583
ACCACGA1551.21001E-714.322588
GGTATAC1051.6557079E-414.0952381
GCTTTAT6200.014.0241931
ATTACAC1601.7831553E-713.8753
Produced by FastQC (version 0.11.5)