##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630059.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1189404
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85915130603227	34.0	31.0	34.0	31.0	34.0
2	33.08501989231581	34.0	33.0	34.0	31.0	34.0
3	33.212363503065404	34.0	33.0	34.0	31.0	34.0
4	36.49635952123921	37.0	37.0	37.0	35.0	37.0
5	36.44063497348252	37.0	37.0	37.0	35.0	37.0
6	36.48485123641757	37.0	37.0	37.0	35.0	37.0
7	36.415706521921905	37.0	37.0	37.0	35.0	37.0
8	36.41505241280507	37.0	37.0	37.0	35.0	37.0
9	38.18756705038826	39.0	39.0	39.0	37.0	39.0
10	38.17335825337732	39.0	39.0	39.0	37.0	39.0
11	38.199055997793856	39.0	39.0	39.0	37.0	39.0
12	38.1413926638888	39.0	39.0	39.0	37.0	39.0
13	38.15659607669051	39.0	39.0	39.0	37.0	39.0
14	39.60755470807228	41.0	40.0	41.0	37.0	41.0
15	39.60399662351901	41.0	40.0	41.0	37.0	41.0
16	39.508418502039675	41.0	39.0	41.0	37.0	41.0
17	39.45511365356094	41.0	39.0	41.0	37.0	41.0
18	39.34847789312967	41.0	39.0	41.0	36.0	41.0
19	39.34549404575737	41.0	39.0	41.0	36.0	41.0
20	39.585213266476316	41.0	39.0	41.0	37.0	41.0
21	39.58863514836002	41.0	40.0	41.0	37.0	41.0
22	39.60318697431655	41.0	40.0	41.0	37.0	41.0
23	39.54911451449634	41.0	40.0	41.0	37.0	41.0
24	39.511726881698735	41.0	40.0	41.0	37.0	41.0
25	39.48982683764306	41.0	40.0	41.0	37.0	41.0
26	39.33527464175335	41.0	39.0	41.0	36.0	41.0
27	39.25339581840989	41.0	39.0	41.0	36.0	41.0
28	39.197973102495034	41.0	39.0	41.0	36.0	41.0
29	39.10991807661652	41.0	39.0	41.0	36.0	41.0
30	39.02432646939139	41.0	39.0	41.0	35.0	41.0
31	38.88646246355317	40.0	39.0	41.0	35.0	41.0
32	38.8271537677694	40.0	39.0	41.0	35.0	41.0
33	38.74925592986067	40.0	39.0	41.0	35.0	41.0
34	38.689677351009415	40.0	38.0	41.0	35.0	41.0
35	38.55705798870695	40.0	38.0	41.0	35.0	41.0
36	38.5458498542127	40.0	38.0	41.0	35.0	41.0
37	38.49612158694607	40.0	38.0	41.0	35.0	41.0
38	38.40766131608772	40.0	38.0	41.0	35.0	41.0
39	38.355067748216754	40.0	38.0	41.0	34.0	41.0
40	38.288408312062174	40.0	38.0	41.0	34.0	41.0
41	38.195709784059915	40.0	38.0	41.0	34.0	41.0
42	38.109178210263295	40.0	38.0	41.0	34.0	41.0
43	37.81572619564084	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	5.0
16	8.0
17	6.0
18	20.0
19	61.0
20	110.0
21	230.0
22	430.0
23	719.0
24	1157.0
25	1865.0
26	2597.0
27	3728.0
28	5108.0
29	6843.0
30	8825.0
31	11216.0
32	14664.0
33	19417.0
34	26505.0
35	37475.0
36	57385.0
37	100583.0
38	220555.0
39	669890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.992621514640945	18.807234547723063	11.987011982471893	25.213131955164098
2	18.97412485581014	21.10283806007042	34.11523754754482	25.80779953657462
3	19.714663814818177	22.84589592770833	30.19066692225686	27.24877333521663
4	14.341636651633927	15.269328167720978	35.761440183486854	34.62759499715824
5	14.337685092701891	36.3920921738955	35.10287505338808	14.167347680014528
6	34.63213508614398	35.49239787322054	14.951521938718887	14.92394510191659
7	28.74683454906827	30.25733896976973	21.97882300715316	19.01700347400883
8	27.338986584877805	32.9532269943602	20.276878167552827	19.43090825320917
9	25.924917017262427	14.501128296188678	20.292095873227264	39.28185881332163
10	16.804887153565986	26.992426458966	34.125999239955476	22.076687147512537
11	34.55940958665012	22.50606185955319	22.00589538962371	20.92863316417298
12	21.860696617801857	25.50428618030543	29.86386459100524	22.77115261088747
13	30.6659469784867	20.290750661675933	24.727426509411437	24.31587585042593
14	21.932833587242015	21.18960420513131	26.190764450094335	30.686797757532343
15	24.443418720636554	28.73548432660391	22.72911474990836	24.091982202851174
16	23.598205487790523	27.25062300109971	24.76097272247277	24.390198788637
17	23.377927096259977	26.795521118139842	26.103746077867573	23.72280570773261
18	23.177658726555485	25.97981846370115	27.18151275765005	23.661010052093317
19	24.80191759906642	25.250041197103762	27.68479002929198	22.263251174537835
20	24.83008296592243	25.666047869353054	26.342689279672843	23.161179885051673
21	23.687157601622324	25.623085175432404	26.978722116286814	23.71103510665846
22	23.91475058096324	25.852611896378352	27.146453181593472	23.086184341064936
23	23.880279534960366	25.70648829161496	26.333945404589187	24.079286768835484
24	23.45191373158321	25.991000534721593	27.107021667995063	23.450064065700133
25	24.038173740797912	26.090630265242087	26.38733348803266	23.483862505927338
26	23.774260049571044	26.173444851370935	26.725654193192554	23.32664090586546
27	23.581642570564753	25.697324037921515	26.855887486505846	23.865145905007886
28	23.54851673611321	26.007731603391278	26.511597405086917	23.93215425540859
29	23.830170404673265	26.29510242104449	26.93491866514658	22.939808509135666
30	24.19077117615209	25.888596305376478	26.122158660976424	23.798473857495015
31	23.030526213128592	26.410622463015088	27.30628112903605	23.25257019482026
32	22.994037349798724	25.96636634818783	27.13468258051932	23.904913721494125
33	22.630998382383112	26.435340725270805	27.17083514096136	23.762825751384728
34	24.013539554264153	24.75937528375556	27.1979075234319	24.029177638548383
35	23.129315186429505	25.151252223802846	27.365638588738562	24.353794001029087
36	22.977894811182743	25.545903662674753	27.83494926870937	23.641252257433134
37	22.94384414378966	24.363294557610367	28.50436016694075	24.18850113165922
38	23.203974427528408	24.93837249580462	28.05547988740579	23.802173189261175
39	22.565923773587443	24.681100786612458	28.65384680058248	24.099128639217625
40	21.849766773947287	24.279891441427807	29.198489327427858	24.671852457197048
41	21.209782378401286	24.470407027385143	29.544124620398115	24.775685973815456
42	21.31025286614136	24.543805132654676	29.73606949362874	24.409872507575223
43	21.573325800148645	24.45535747315462	29.682176955853517	24.289139770843214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1310.0
1	1032.0
2	754.0
3	1337.0
4	1920.0
5	1920.0
6	2105.0
7	2290.0
8	1839.5
9	1389.0
10	2051.0
11	2713.0
12	2713.0
13	4202.0
14	5691.0
15	7196.0
16	8701.0
17	9276.0
18	9851.0
19	9851.0
20	12087.5
21	14324.0
22	14753.0
23	15182.0
24	18103.0
25	21024.0
26	21024.0
27	24089.0
28	27154.0
29	30415.5
30	33677.0
31	37499.0
32	41321.0
33	41321.0
34	44927.0
35	48533.0
36	52624.5
37	56716.0
38	58416.5
39	60117.0
40	60117.0
41	62478.0
42	64839.0
43	65548.0
44	66257.0
45	68128.0
46	69999.0
47	69999.0
48	72466.5
49	74934.0
50	76248.0
51	77562.0
52	77298.0
53	77034.0
54	77034.0
55	74210.0
56	71386.0
57	70184.5
58	68983.0
59	67695.0
60	66407.0
61	66407.0
62	61641.5
63	56876.0
64	52439.0
65	48002.0
66	41210.0
67	34418.0
68	34418.0
69	30660.5
70	26903.0
71	22368.0
72	17833.0
73	12512.5
74	7192.0
75	7192.0
76	5523.5
77	3855.0
78	2978.0
79	2101.0
80	1614.0
81	1127.0
82	1127.0
83	837.5
84	548.0
85	404.0
86	260.0
87	191.0
88	122.0
89	122.0
90	89.5
91	57.0
92	41.5
93	26.0
94	18.0
95	10.0
96	10.0
97	7.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1189404.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.9090708179592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.48687913529686	57.66178912570518
2	7.8478557549269565	10.344897614412188
3	1.9386634664382507	3.8332652310500674
4	0.7933899167507944	2.0916636883753146
5	0.4285514633460695	1.412271437340807
6	0.2802082525992654	1.108095933860495
7	0.19027263727214003	0.8778484907282539
8	0.14633526287612095	0.7715856963253545
9	0.11055181184240644	0.655773047619738
>10	0.6787844036928181	8.35109841993062
>50	0.05011133311635514	2.2885929132848744
>100	0.04097064038691601	5.89061320537007
>500	0.005377391398537028	2.3500502677647415
>1k	0.0020485300565855347	2.3624549282323137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3631	0.305278946430313	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3288	0.276440973798642	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2733	0.22977894811182742	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2095	0.17613863750248024	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2095	0.17613863750248024	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1905	0.1601642503304176	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1584	0.13317594358182755	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1567	0.1317466563085377	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1333	0.11207293737031321	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1255	0.10551503105757169	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1193	0.10030233629616178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	8.407572195822446E-5	0.0	0.0	0.0	0.0
3	8.407572195822446E-5	0.0	0.0	0.0	0.0
4	8.407572195822446E-5	0.0	0.0	0.0	0.0
5	6.726057756657956E-4	0.0	0.0	0.0	0.0
6	6.726057756657956E-4	0.0	0.0	0.0	0.0
7	6.726057756657956E-4	0.0	0.0	0.0	0.0
8	6.726057756657956E-4	0.0	0.0	0.0	0.0
9	6.726057756657956E-4	0.0	0.0	2.5222716587467337E-4	0.0
10	8.407572195822446E-4	0.0	0.0	3.363028878328978E-4	0.0
11	9.24832941540469E-4	0.0	0.0	3.363028878328978E-4	0.0
12	0.0011770601074151423	0.0	0.0	4.203786097911223E-4	0.0
13	0.0011770601074151423	0.0	0.0	5.044543317493467E-4	0.0
14	0.0011770601074151423	0.0	0.0	5.044543317493467E-4	0.0
15	0.0016815144391644891	0.0	0.0	5.885300537075711E-4	0.0
16	0.0016815144391644891	0.0	0.0	8.407572195822446E-4	0.0
17	0.0021018930489556113	0.0	0.0	8.407572195822446E-4	0.0
18	0.00227004449287206	0.0	0.0	0.0010089086634986935	0.0
19	0.0026904231026631826	0.0	0.0	0.0011770601074151423	0.0
20	0.0026904231026631826	0.0	0.0	0.00151336299524804	0.0
21	0.002774498824621407	0.0	0.0	0.002017817326997387	0.0
22	0.002942650268537856	0.0	0.0	0.002606347380704958	0.0
23	0.002942650268537856	0.0	0.0	0.004203786097911223	0.0
24	0.002942650268537856	0.0	0.0	0.008407572195822445	0.0
25	0.002942650268537856	0.0	0.0	0.01378841840114881	0.0
26	0.002942650268537856	0.0	0.0	0.02530679230942556	0.0
27	0.003110801712454305	0.0	0.0	0.09794821608133149	0.0
28	0.003699331766161876	0.0	0.0	0.22994709955574388	0.0
29	0.003699331766161876	8.407572195822446E-5	0.0	0.40171379951639646	0.0
30	0.003867483210078325	8.407572195822446E-5	0.0	0.5557405221438636	0.0
31	0.0039515589320365495	8.407572195822446E-5	0.0	0.8827110048393986	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	960	0.0	28.713541	1
CTAGTAC	40	0.0019312269	23.125	3
CGTGCGA	80	6.960545E-7	20.8125	10
CGAATTA	105	2.2595486E-8	19.38095	15
AAGCTCG	50	0.0070350235	18.499998	18
GTATCAA	1550	0.0	17.783873	2
CGAGAAT	125	8.578354E-9	17.76	14
GTAGCAC	65	0.001579978	17.076923	3
GTCTAAT	100	5.878137E-6	16.65	1
CGACCAA	125	1.657736E-7	16.279999	12
AAACCGT	70	0.0025925648	15.857143	6
CGGTCGT	85	5.364344E-4	15.235294	10
AGTCCGT	135	3.9721817E-7	15.074075	6
CCGTGCG	125	2.95877E-6	14.799999	9
ACCATCG	140	5.994825E-7	14.535715	30
ATAGGAC	155	1.21001E-7	14.32258	3
ACCACGA	155	1.21001E-7	14.32258	8
GGTATAC	105	1.6557079E-4	14.095238	1
GCTTTAT	620	0.0	14.024193	1
ATTACAC	160	1.7831553E-7	13.875	3
>>END_MODULE