FastQCFastQC Report
Fri 10 Feb 2017
ERR1630058.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630058.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260605
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35260.27970696609961093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.24765886221298503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25630.20331507490451012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.13636309549779668No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT14620.11597605911447281No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG14170.11240634457264569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.026.930381
ACAGCGT701.2193162E-418.58
CCGCGAA609.2374935E-418.527
TAAGCGT902.152552E-618.54
CGACCAA1156.406299E-817.69565212
TCGTTGT1201.0414078E-716.95833234
GTATCAA13200.016.1174242
ACAGCGC1504.671165E-916.0333338
ACAACGT700.002592680515.8571426
GTTATAC700.002592680515.8571421
AACCACG1659.731593E-1015.6969697
TTATACT2201.8189894E-1215.1363644
GGCCTAT1001.0934968E-414.81
ACCACGA1904.5474735E-1014.6052648
CGTTGTA1405.995644E-714.53571435
CATCGTT1405.995644E-714.53571432
TCAGGAC3600.014.3888893
CTTAGAC908.2747964E-414.3888893
AACAGCG1304.444495E-614.2307697
GTTCTAG3700.014.01
Produced by FastQC (version 0.11.5)