##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630058.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1260605
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.882176415292655	34.0	31.0	34.0	31.0	34.0
2	33.10435465510608	34.0	33.0	34.0	31.0	34.0
3	33.22907413503833	34.0	33.0	34.0	31.0	34.0
4	36.50921184669266	37.0	37.0	37.0	35.0	37.0
5	36.45583588832346	37.0	37.0	37.0	35.0	37.0
6	36.49992820907421	37.0	37.0	37.0	35.0	37.0
7	36.43330464340535	37.0	37.0	37.0	35.0	37.0
8	36.43863145077165	37.0	37.0	37.0	35.0	37.0
9	38.217862851567304	39.0	39.0	39.0	37.0	39.0
10	38.19671110300213	39.0	39.0	39.0	37.0	39.0
11	38.232808849718985	39.0	39.0	39.0	37.0	39.0
12	38.17080132158765	39.0	39.0	39.0	37.0	39.0
13	38.18413856838581	39.0	39.0	39.0	37.0	39.0
14	39.654202545603106	41.0	40.0	41.0	37.0	41.0
15	39.65069232630364	41.0	40.0	41.0	37.0	41.0
16	39.568178771304254	41.0	40.0	41.0	37.0	41.0
17	39.492592842325706	41.0	39.0	41.0	37.0	41.0
18	39.3945835531352	41.0	39.0	41.0	36.0	41.0
19	39.42001578607098	41.0	39.0	41.0	37.0	41.0
20	39.63412250467038	41.0	40.0	41.0	37.0	41.0
21	39.64858698799386	41.0	40.0	41.0	37.0	41.0
22	39.6632013993281	41.0	40.0	41.0	37.0	41.0
23	39.60123036161208	41.0	40.0	41.0	37.0	41.0
24	39.568419132083406	41.0	40.0	41.0	37.0	41.0
25	39.54028502187442	41.0	40.0	41.0	37.0	41.0
26	39.40094478444874	41.0	40.0	41.0	37.0	41.0
27	39.326684409470055	41.0	39.0	41.0	36.0	41.0
28	39.29252938073385	41.0	39.0	41.0	36.0	41.0
29	39.199109951174236	41.0	39.0	41.0	36.0	41.0
30	39.11759115662718	41.0	39.0	41.0	36.0	41.0
31	38.995599731874776	40.0	39.0	41.0	35.0	41.0
32	38.912603868777296	40.0	39.0	41.0	35.0	41.0
33	38.8449442926214	40.0	39.0	41.0	35.0	41.0
34	38.782356090924594	40.0	39.0	41.0	35.0	41.0
35	38.658640097413546	40.0	38.0	41.0	35.0	41.0
36	38.65362028549784	40.0	38.0	41.0	35.0	41.0
37	38.61618349919285	40.0	38.0	41.0	35.0	41.0
38	38.52500109074611	40.0	38.0	41.0	35.0	41.0
39	38.48335442109146	40.0	38.0	41.0	35.0	41.0
40	38.43443901935975	40.0	38.0	41.0	35.0	41.0
41	38.33908004489908	40.0	38.0	41.0	35.0	41.0
42	38.25851079442014	40.0	38.0	41.0	34.0	41.0
43	37.97264488083103	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	3.0
16	9.0
17	13.0
18	42.0
19	68.0
20	125.0
21	248.0
22	435.0
23	763.0
24	1162.0
25	1889.0
26	2608.0
27	3571.0
28	4948.0
29	6676.0
30	8829.0
31	11590.0
32	14844.0
33	19452.0
34	26630.0
35	37456.0
36	57249.0
37	102421.0
38	223983.0
39	735586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01632946085412	19.247266193613385	12.46583981500946	25.270564530523043
2	18.329056286465626	21.560203235747917	35.957258617885856	24.1534818599006
3	18.887597621776845	23.55638760753765	31.521610655201272	26.034404115484232
4	14.550632434426328	16.029366851630762	35.60409485921443	33.815905854728484
5	13.837006834020174	37.084574470194866	35.33803213536358	13.740386560421387
6	33.665343228053196	35.83969601897501	15.668905009896042	14.826055743075745
7	27.697573784016406	31.174079112806947	22.55258387837586	18.575763224800788
8	25.595091245870037	35.15359688403584	20.629856299157943	18.621455570936178
9	25.585968642040925	15.112584830299735	20.425589300375613	38.875857227283724
10	16.10813855252042	27.707648311723336	34.800036490415316	21.384176645340926
11	34.10838446618885	22.545127141332934	22.699735444488955	20.64675294798926
12	20.063064956905613	26.048682973651538	30.897307245330612	22.99094482411223
13	28.953161378861736	21.036803756926236	25.80625969276657	24.203775171445457
14	21.58479460259161	21.954141067185994	26.38574335338984	30.075320976832554
15	23.858544111755865	29.13870720804693	23.574315507236605	23.428433172960602
16	23.495067844408045	27.87859797478195	25.059396083626513	23.566938097183495
17	22.774858103846963	27.732477659536492	26.605003153247846	22.887661083368698
18	22.19481915429496	27.00901551239286	27.52575152406979	23.27041380924239
19	23.53600057115433	26.58160169125142	28.11824481102328	21.764152926570972
20	23.617390062707987	26.295707219945978	27.87970855263941	22.207194164706628
21	22.546396373170026	27.030909761582734	27.1520420750354	23.270651790211843
22	23.083678075209917	26.493310751583564	27.126181476354606	23.29682969685191
23	22.76042059170002	26.988231841060443	27.064623732255544	23.186723834983997
24	23.52061113512956	26.71812344072886	27.116979545535674	22.644285878605906
25	23.532748164571775	26.06788010518759	27.45245338547761	22.94691834476303
26	23.33681049972037	26.48720257336755	27.286501322777557	22.889485604134524
27	22.969129901912176	26.634433466470465	27.016234268466334	23.380202363151025
28	22.748283562257804	26.65283732810833	27.497669769674083	23.10120933995978
29	23.14253870165516	26.922231785531554	27.28872247849247	22.646507034320823
30	22.654757041261934	27.536064032746182	26.99291213345973	22.816266792532158
31	23.21750270703353	26.465625632136952	27.509727472126478	22.80714418870304
32	22.455170334878886	26.83766921438516	27.532811626163628	23.174348824572327
33	22.79937014370084	26.50719297480178	27.461893297265995	23.23154358423138
34	22.804050436100127	26.057726250490838	27.686309351462196	23.451913961946843
35	22.865528853209373	26.18782251379298	27.757069026380186	23.189579606617457
36	22.764624922160394	25.98069974337719	27.95467255801778	23.300002776444646
37	22.91693274261168	24.92826856945673	28.28038917821205	23.87440950971954
38	22.377112576897602	25.47411758639701	28.991317660964377	23.157452175741014
39	22.131833524379164	25.290078970018364	29.33274102514269	23.24534648045978
40	21.863153009864313	24.700600108677975	29.845907322277792	23.590339559179917
41	21.347686230024472	25.100090829403342	29.82559961288429	23.726623327687896
42	21.375371349471088	24.770963148646878	29.61046481649684	24.24320068538519
43	20.979688324257005	25.005295076570377	29.90738573938704	24.10763085978558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	949.0
1	771.0
2	593.0
3	1084.0
4	1575.0
5	1575.0
6	1785.0
7	1995.0
8	1747.0
9	1499.0
10	2079.0
11	2659.0
12	2659.0
13	4048.0
14	5437.0
15	7399.5
16	9362.0
17	9872.5
18	10383.0
19	10383.0
20	12093.5
21	13804.0
22	15116.0
23	16428.0
24	18691.0
25	20954.0
26	20954.0
27	24468.5
28	27983.0
29	34374.5
30	40766.0
31	43220.0
32	45674.0
33	45674.0
34	49525.0
35	53376.0
36	58177.5
37	62979.0
38	68071.5
39	73164.0
40	73164.0
41	79244.0
42	85324.0
43	82332.0
44	79340.0
45	82341.0
46	85342.0
47	85342.0
48	86793.0
49	88244.0
50	91267.5
51	94291.0
52	95599.0
53	96907.0
54	96907.0
55	86738.5
56	76570.0
57	70574.5
58	64579.0
59	57726.5
60	50874.0
61	50874.0
62	46499.0
63	42124.0
64	37717.5
65	33311.0
66	29647.0
67	25983.0
68	25983.0
69	21863.5
70	17744.0
71	15185.0
72	12626.0
73	10327.0
74	8028.0
75	8028.0
76	6178.5
77	4329.0
78	3610.5
79	2892.0
80	2083.5
81	1275.0
82	1275.0
83	960.5
84	646.0
85	484.5
86	323.0
87	226.0
88	129.0
89	129.0
90	103.5
91	78.0
92	57.5
93	37.0
94	29.0
95	21.0
96	21.0
97	14.5
98	8.0
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1260605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.07976620454235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.29622485150699	48.95905031301059
2	9.187206801317108	10.671816461865582
3	2.6137258047156977	4.554137509820011
4	1.1290120652532118	2.6229102716805626
5	0.6117941033239385	1.7766429243185988
6	0.3811338916647613	1.3281700392310023
7	0.2643024372156537	1.074543663254313
8	0.19394172895879647	0.9011272220185296
9	0.1510920193611488	0.7897850243880937
>10	0.9780856938660716	11.006435934302901
>50	0.1110390540684311	4.454995940804673
>100	0.07624811731299806	8.42416583524903
>500	0.0048171133385537645	1.9941692116645648
>1k	0.001376318096729647	1.4420496483917404
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3526	0.27970696609961093	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3122	0.24765886221298503	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2563	0.20331507490451012	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1719	0.13636309549779668	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1462	0.11597605911447281	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1417	0.11240634457264569	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	7.932698981838086E-5	0.0	0.0	0.0	0.0
4	7.932698981838086E-5	0.0	0.0	7.932698981838086E-5	0.0
5	7.932698981838086E-5	0.0	0.0	7.932698981838086E-5	0.0
6	7.932698981838086E-5	0.0	0.0	7.932698981838086E-5	0.0
7	7.932698981838086E-5	0.0	0.0	7.932698981838086E-5	0.0
8	7.932698981838086E-5	0.0	0.0	7.932698981838086E-5	0.0
9	7.932698981838086E-5	0.0	0.0	2.3798096945514257E-4	0.0
10	7.932698981838086E-5	0.0	0.0	3.173079592735234E-4	0.0
11	1.586539796367617E-4	7.932698981838086E-5	0.0	3.173079592735234E-4	0.0
12	1.586539796367617E-4	7.932698981838086E-5	0.0	4.7596193891028514E-4	0.0
13	1.586539796367617E-4	7.932698981838086E-5	0.0	5.55288928728666E-4	0.0
14	1.586539796367617E-4	7.932698981838086E-5	0.0	5.55288928728666E-4	0.0
15	1.586539796367617E-4	7.932698981838086E-5	0.0	5.55288928728666E-4	0.0
16	1.586539796367617E-4	7.932698981838086E-5	0.0	7.139429083654278E-4	0.0
17	1.586539796367617E-4	7.932698981838086E-5	0.0	7.139429083654278E-4	0.0
18	1.586539796367617E-4	7.932698981838086E-5	0.0	7.932698981838086E-4	0.0
19	1.586539796367617E-4	7.932698981838086E-5	0.0	0.0010312508676389512	0.0
20	1.586539796367617E-4	7.932698981838086E-5	0.0	0.0012692318370940937	0.0
21	1.586539796367617E-4	7.932698981838086E-5	0.0	0.001665866786185998	0.0
22	1.586539796367617E-4	7.932698981838086E-5	0.0	0.0025384636741881874	0.0
23	1.586539796367617E-4	7.932698981838086E-5	0.0	0.003887022501100662	0.0
24	1.586539796367617E-4	7.932698981838086E-5	0.0	0.00737741005310942	0.0
25	1.586539796367617E-4	7.932698981838086E-5	0.0	0.011819721482938748	0.0
26	1.586539796367617E-4	7.932698981838086E-5	0.0	0.0203077093935055	0.0
27	1.586539796367617E-4	7.932698981838086E-5	0.0	0.08440391716675723	0.0
28	1.586539796367617E-4	7.932698981838086E-5	0.0	0.20006266832195652	0.0
29	1.586539796367617E-4	7.932698981838086E-5	0.0	0.32555796621463506	0.0
30	1.586539796367617E-4	7.932698981838086E-5	0.0	0.46398356344770963	0.0
31	1.586539796367617E-4	7.932698981838086E-5	0.0	0.7460703392418719	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	790	0.0	26.93038	1
ACAGCGT	70	1.2193162E-4	18.5	8
CCGCGAA	60	9.2374935E-4	18.5	27
TAAGCGT	90	2.152552E-6	18.5	4
CGACCAA	115	6.406299E-8	17.695652	12
TCGTTGT	120	1.0414078E-7	16.958332	34
GTATCAA	1320	0.0	16.117424	2
ACAGCGC	150	4.671165E-9	16.033333	8
ACAACGT	70	0.0025926805	15.857142	6
GTTATAC	70	0.0025926805	15.857142	1
AACCACG	165	9.731593E-10	15.696969	7
TTATACT	220	1.8189894E-12	15.136364	4
GGCCTAT	100	1.0934968E-4	14.8	1
ACCACGA	190	4.5474735E-10	14.605264	8
CGTTGTA	140	5.995644E-7	14.535714	35
CATCGTT	140	5.995644E-7	14.535714	32
TCAGGAC	360	0.0	14.388889	3
CTTAGAC	90	8.2747964E-4	14.388889	3
AACAGCG	130	4.444495E-6	14.230769	7
GTTCTAG	370	0.0	14.0	1
>>END_MODULE