FastQCFastQC Report
Fri 10 Feb 2017
ERR1630057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849266
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25980.30591122216125455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.26705413851490584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17740.20888626178370498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.1434179632765235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.027.0504191
ATAGGCC555.1415735E-420.1818188
TCCGCGA902.1508695E-618.526
CGAGAGA902.1508695E-618.510
CCGCGAA852.7217598E-517.41176427
CTTAGGG752.0664654E-417.2666663
GCACCGA1301.3915269E-817.0769236
GTATCAA9750.016.5076942
CGAATTA1606.2755134E-1016.187515
ACCGTTT1059.337267E-615.8571448
TGCACCG1059.337267E-615.8571445
CTACACT1455.3389158E-815.3103464
GTGTAGT1101.45090125E-515.1363641
GGCTACG1101.45090125E-515.1363641
CTTATAC7200.014.90277837
GTATTAT1252.956358E-614.8000011
ACCGAGA1252.956358E-614.8000018
CACCGTT1252.956358E-614.8000017
AGACCGT750.00410323214.86
CGGTCGA750.00410323214.820
Produced by FastQC (version 0.11.5)