##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630057.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	849266
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87678418775743	34.0	31.0	34.0	31.0	34.0
2	33.09846620493462	34.0	33.0	34.0	31.0	34.0
3	33.22296430093751	34.0	33.0	34.0	31.0	34.0
4	36.50858977046061	37.0	37.0	37.0	35.0	37.0
5	36.45625752120066	37.0	37.0	37.0	35.0	37.0
6	36.49293625318805	37.0	37.0	37.0	35.0	37.0
7	36.42750681176451	37.0	37.0	37.0	35.0	37.0
8	36.430052539487036	37.0	37.0	37.0	35.0	37.0
9	38.20958333431457	39.0	39.0	39.0	37.0	39.0
10	38.18935645604557	39.0	39.0	39.0	37.0	39.0
11	38.22157839828746	39.0	39.0	39.0	37.0	39.0
12	38.16341287653103	39.0	39.0	39.0	37.0	39.0
13	38.17324489618094	39.0	39.0	39.0	37.0	39.0
14	39.63446081675235	41.0	40.0	41.0	37.0	41.0
15	39.62777033344088	41.0	40.0	41.0	37.0	41.0
16	39.54447605343909	41.0	40.0	41.0	37.0	41.0
17	39.46709393758846	41.0	39.0	41.0	37.0	41.0
18	39.36270261614147	41.0	39.0	41.0	36.0	41.0
19	39.38352883548853	41.0	39.0	41.0	36.0	41.0
20	39.6039768458881	41.0	40.0	41.0	37.0	41.0
21	39.619246502273725	41.0	40.0	41.0	37.0	41.0
22	39.638778662986624	41.0	40.0	41.0	37.0	41.0
23	39.5640141015889	41.0	40.0	41.0	37.0	41.0
24	39.530761857886695	41.0	40.0	41.0	37.0	41.0
25	39.50489128258991	41.0	40.0	41.0	37.0	41.0
26	39.36532959049344	41.0	39.0	41.0	36.0	41.0
27	39.288426712007784	41.0	39.0	41.0	36.0	41.0
28	39.24585583315475	41.0	39.0	41.0	36.0	41.0
29	39.12505504753516	41.0	39.0	41.0	36.0	41.0
30	39.03997922912256	41.0	39.0	41.0	35.0	41.0
31	38.913444079946686	40.0	39.0	41.0	35.0	41.0
32	38.82829996726585	40.0	39.0	41.0	35.0	41.0
33	38.7580498924954	40.0	39.0	41.0	35.0	41.0
34	38.67709998987361	40.0	38.0	41.0	35.0	41.0
35	38.56105154333272	40.0	38.0	41.0	35.0	41.0
36	38.544554945093765	40.0	38.0	41.0	35.0	41.0
37	38.50071355735423	40.0	38.0	41.0	35.0	41.0
38	38.39332553051694	40.0	38.0	41.0	35.0	41.0
39	38.33858885202045	40.0	38.0	41.0	34.0	41.0
40	38.278518155677965	40.0	38.0	41.0	34.0	41.0
41	38.18735355000671	40.0	38.0	41.0	34.0	41.0
42	38.097246328005596	40.0	38.0	41.0	34.0	41.0
43	37.79955867772877	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	5.0
16	6.0
17	14.0
18	30.0
19	47.0
20	83.0
21	192.0
22	336.0
23	570.0
24	879.0
25	1352.0
26	1895.0
27	2679.0
28	3523.0
29	4782.0
30	6223.0
31	8093.0
32	10250.0
33	13659.0
34	18692.0
35	26464.0
36	40361.0
37	70612.0
38	153871.0
39	484643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.682269159485955	19.470107127802123	12.435326505476493	25.412297207235422
2	18.6842520482393	20.845530140144547	35.8953496313287	24.57486818028745
3	18.88395390843387	23.033419446910628	31.201296178111456	26.88133046654405
4	14.134911794420123	16.02324831089435	36.11801249549611	33.72382739918942
5	14.38230189363521	36.353392223402324	34.88942215984156	14.374883723120908
6	33.55897916553824	36.35986840401005	15.542833458539492	14.538318971912215
7	28.271001076223467	30.96886016866329	22.17114543617665	18.588993318936588
8	26.039780233754794	34.69219302315176	20.214985646428797	19.05304109666465
9	25.758007502949603	14.789359282015294	20.73402208495336	38.71861113008174
10	16.52721291091366	27.099518878655214	34.42984883416974	21.94341937626138
11	34.466115445573	22.31385690702324	22.581382040491434	20.638645606912323
12	20.465437212840264	25.618710745514363	31.00559777501984	22.910254266625532
13	29.395501527201134	20.67220399733417	25.342707702886962	24.589586772577732
14	21.317231585863556	21.767856007422882	26.822338348644593	30.092574058068966
15	24.1933622681233	28.575263816048214	23.37971848631642	23.85165542951207
16	23.270447657153355	27.7878780028872	25.08778168441925	23.853892655540196
17	22.597160371426618	27.3416102846458	26.987422079772415	23.073807264155164
18	22.75223545979705	26.462262706855093	27.4711338968003	23.31436793654756
19	23.46944302491799	26.27822142885739	27.774101400503493	22.478234145721128
20	23.471327004731144	26.43447400461104	27.337606827542842	22.756592163114973
21	23.467205798889864	26.31354605035407	27.154978534405004	23.06426961635106
22	23.481217898750213	26.503710262744534	27.273433765157208	22.741638073348046
23	23.44259631258051	26.220642295817797	27.158628745292994	23.178132646308693
24	23.357817220988476	26.217698577359744	27.214088401042787	23.210395800608996
25	23.504532148938022	25.830305228279478	27.371518464179655	23.29364415860284
26	23.36370465790459	26.383959795870787	27.26943030805425	22.982905238170375
27	23.082638419529335	26.79761111359692	27.04900466991496	23.070745796958786
28	23.14151278869047	26.601323966813695	27.228571495856425	23.028591748639414
29	22.88305430807309	26.56258463190567	27.757498828400053	22.796862231621187
30	23.07416051037013	26.771353144951053	27.173818332536566	22.98066801214225
31	23.08499339429578	26.355935596150086	27.312761843756846	23.24630916579729
32	22.885880277792822	26.259734876940794	27.828265820131737	23.026119025134644
33	22.84996691260453	26.328971135074287	27.698977705453885	23.122084246867296
34	23.204508363692884	25.751060327388593	27.44605341553765	23.598377893380874
35	22.85196864115601	25.67263966766596	28.009834374624674	23.465557316553355
36	23.12137775443736	25.435375959946587	27.738659030268494	23.70458725534756
37	22.69265459820598	24.67966455739427	28.66074940007018	23.96693144432957
38	22.487654044786908	24.904800145066446	29.164949497566134	23.44259631258051
39	21.869355419856674	25.053045806614183	29.596380874778927	23.481217898750213
40	21.820607442191257	24.51799553967779	29.997433077504574	23.663963940626374
41	21.395416748109543	24.672599633094933	29.89899513226716	24.032988486528367
42	21.01791429304835	25.16891056512329	29.80161692567464	24.011558216153713
43	21.144847432959757	24.740422906368558	29.992016635541752	24.122713025129936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	824.0
1	637.0
2	450.0
3	759.0
4	1068.0
5	1068.0
6	1216.0
7	1364.0
8	1160.0
9	956.0
10	1340.5
11	1725.0
12	1725.0
13	2812.5
14	3900.0
15	4930.0
16	5960.0
17	6329.5
18	6699.0
19	6699.0
20	9071.0
21	11443.0
22	11960.0
23	12477.0
24	14855.0
25	17233.0
26	17233.0
27	18947.0
28	20661.0
29	22966.5
30	25272.0
31	28431.5
32	31591.0
33	31591.0
34	34807.0
35	38023.0
36	41154.0
37	44285.0
38	45674.5
39	47064.0
40	47064.0
41	48871.5
42	50679.0
43	51750.5
44	52822.0
45	52843.0
46	52864.0
47	52864.0
48	53142.0
49	53420.0
50	54237.5
51	55055.0
52	55378.5
53	55702.0
54	55702.0
55	53573.0
56	51444.0
57	48690.5
58	45937.0
59	43092.0
60	40247.0
61	40247.0
62	37652.5
63	35058.0
64	30730.5
65	26403.0
66	22893.5
67	19384.0
68	19384.0
69	16503.0
70	13622.0
71	11458.0
72	9294.0
73	8088.0
74	6882.0
75	6882.0
76	5670.0
77	4458.0
78	3519.5
79	2581.0
80	1912.5
81	1244.0
82	1244.0
83	920.0
84	596.0
85	453.0
86	310.0
87	233.5
88	157.0
89	157.0
90	111.0
91	65.0
92	42.5
93	20.0
94	18.0
95	16.0
96	16.0
97	11.0
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	849266.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.64521672493487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.58881679162926	63.6290857850852
2	8.265353313173108	12.008767654872377
3	1.9105918080764217	4.163860679117905
4	0.7592925805794608	2.2063589629532
5	0.3681290288599855	1.3371406542136728
6	0.2318061344946732	1.010376412712096
7	0.13637746782937482	0.6935019494803936
8	0.09586274024596451	0.5571175632811383
9	0.07514185822450324	0.49128269182500317
>10	0.49270988971655855	6.949136515890331
>50	0.047899045451448574	2.4413707013520582
>100	0.027204825996269732	3.5120160857036313
>500	1.6290314457408745E-4	0.07005807471712724
>1k	6.516125782963498E-4	0.9299262687959222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2598	0.30591122216125455	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2268	0.26705413851490584	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1774	0.20888626178370498	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1218	0.1434179632765235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	8.242411682558822E-4	0.0
13	0.0	0.0	0.0	8.242411682558822E-4	0.0
14	0.0	0.0	0.0	8.242411682558822E-4	0.0
15	0.0	0.0	0.0	8.242411682558822E-4	0.0
16	0.0	0.0	0.0	9.41989906578151E-4	0.0
17	0.0	0.0	0.0	0.0011774873832226887	0.0
18	0.0	0.0	0.0	0.0011774873832226887	0.0
19	0.0	0.0	0.0	0.0011774873832226887	0.0
20	0.0	0.0	0.0	0.0014129848598672266	0.0
21	0.0	0.0	0.0	0.0016484823365117644	0.0
22	0.0	0.0	0.0	0.0023549747664453773	0.0
23	0.0	0.0	0.0	0.004945447009535293	0.0
24	0.0	0.0	0.0	0.010244140234037392	0.0
25	1.1774873832226888E-4	0.0	0.0	0.016602572103439912	0.0
26	1.1774873832226888E-4	0.0	0.0	0.03061467196378991	0.0
27	1.1774873832226888E-4	0.0	0.0	0.09584747299432686	0.0
28	1.1774873832226888E-4	0.0	0.0	0.21960139697103145	0.0
29	1.1774873832226888E-4	0.0	0.0	0.35925140062124233	0.0
30	1.1774873832226888E-4	0.0	0.0	0.526807855253831	0.0
31	1.1774873832226888E-4	0.0	0.0	0.8540315990514161	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	595	0.0	27.050419	1
ATAGGCC	55	5.1415735E-4	20.181818	8
TCCGCGA	90	2.1508695E-6	18.5	26
CGAGAGA	90	2.1508695E-6	18.5	10
CCGCGAA	85	2.7217598E-5	17.411764	27
CTTAGGG	75	2.0664654E-4	17.266666	3
GCACCGA	130	1.3915269E-8	17.076923	6
GTATCAA	975	0.0	16.507694	2
CGAATTA	160	6.2755134E-10	16.1875	15
ACCGTTT	105	9.337267E-6	15.857144	8
TGCACCG	105	9.337267E-6	15.857144	5
CTACACT	145	5.3389158E-8	15.310346	4
GTGTAGT	110	1.45090125E-5	15.136364	1
GGCTACG	110	1.45090125E-5	15.136364	1
CTTATAC	720	0.0	14.902778	37
GTATTAT	125	2.956358E-6	14.800001	1
ACCGAGA	125	2.956358E-6	14.800001	8
CACCGTT	125	2.956358E-6	14.800001	7
AGACCGT	75	0.004103232	14.8	6
CGGTCGA	75	0.004103232	14.8	20
>>END_MODULE