FastQCFastQC Report
Fri 10 Feb 2017
ERR1630056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732468
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28020.3825423090155474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.3152356143886149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18140.24765587029057923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12220.1668332268440396No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG9980.13625168608048407No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG9380.12806020194738882No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG9200.12560275670746027No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT8710.1189130446654325No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA7660.10457794743251583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6100.028.5081961
CCTTATA806.9530324E-720.81252
GTACGAT450.003824503920.5555556
GTATCAA9200.018.9021742
GTATAAC609.232458E-418.51
ACCGTGT609.232458E-418.58
AGTACGA650.001579194417.0769235
TAGGACA904.442416E-516.4444454
GTCTAAT700.002591289815.8571431
TCTATAG700.002591289815.8571433
AACTGTG2100.015.8571427
GTTATAT1059.333769E-615.8571421
CTCTTAG1101.4503594E-515.1363641
GTATATA1353.9661245E-715.0740751
GGATAGA750.004102515514.81
GTTCTAG908.268966E-414.3888881
GTGTAAG908.268966E-414.3888881
GTATTAG1551.2078453E-714.3225811
CTAGTCC1051.6543377E-414.0952383
ACTGTCC1203.2971475E-513.8749998
Produced by FastQC (version 0.11.5)