##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630056.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732468
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84975589377284	34.0	31.0	34.0	31.0	34.0
2	33.06863371505649	34.0	33.0	34.0	31.0	34.0
3	33.199457177651446	34.0	33.0	34.0	31.0	34.0
4	36.49109176100526	37.0	37.0	37.0	35.0	37.0
5	36.438887432625044	37.0	37.0	37.0	35.0	37.0
6	36.477930776498084	37.0	37.0	37.0	35.0	37.0
7	36.40340055811312	37.0	37.0	37.0	35.0	37.0
8	36.409439047166565	37.0	37.0	37.0	35.0	37.0
9	38.18997553476739	39.0	39.0	39.0	37.0	39.0
10	38.169595122244246	39.0	39.0	39.0	37.0	39.0
11	38.199524074771865	39.0	39.0	39.0	37.0	39.0
12	38.127297028675656	39.0	39.0	39.0	37.0	39.0
13	38.14592036785225	39.0	39.0	39.0	37.0	39.0
14	39.60072248890054	41.0	40.0	41.0	37.0	41.0
15	39.57776995035961	41.0	40.0	41.0	37.0	41.0
16	39.507930721888194	41.0	39.0	41.0	37.0	41.0
17	39.42194334769574	41.0	39.0	41.0	37.0	41.0
18	39.314315437671	41.0	39.0	41.0	36.0	41.0
19	39.3410715553444	41.0	39.0	41.0	36.0	41.0
20	39.56729986839016	41.0	39.0	41.0	37.0	41.0
21	39.57951064073789	41.0	40.0	41.0	37.0	41.0
22	39.60109110568653	41.0	40.0	41.0	37.0	41.0
23	39.54239639137819	41.0	40.0	41.0	37.0	41.0
24	39.50945706843166	41.0	40.0	41.0	37.0	41.0
25	39.47602079544772	41.0	40.0	41.0	37.0	41.0
26	39.3348965415554	41.0	39.0	41.0	36.0	41.0
27	39.24540594264869	41.0	39.0	41.0	36.0	41.0
28	39.20729915846153	41.0	39.0	41.0	36.0	41.0
29	39.10118257725935	41.0	39.0	41.0	36.0	41.0
30	39.02014149423592	40.0	39.0	41.0	35.0	41.0
31	38.87125171338543	40.0	39.0	41.0	35.0	41.0
32	38.775076863426115	40.0	39.0	41.0	35.0	41.0
33	38.70737561231344	40.0	38.0	41.0	35.0	41.0
34	38.60184062648471	40.0	38.0	41.0	35.0	41.0
35	38.50279602658409	40.0	38.0	41.0	35.0	41.0
36	38.47379134651616	40.0	38.0	41.0	35.0	41.0
37	38.405609528334345	40.0	38.0	41.0	35.0	41.0
38	38.330961898676804	40.0	38.0	41.0	34.0	41.0
39	38.25341311838879	40.0	38.0	41.0	34.0	41.0
40	38.18717131669916	40.0	38.0	41.0	34.0	41.0
41	38.087388117979216	40.0	38.0	41.0	34.0	41.0
42	37.990051442520354	40.0	38.0	41.0	34.0	41.0
43	37.69207255470546	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	2.0
16	6.0
17	6.0
18	17.0
19	39.0
20	83.0
21	150.0
22	272.0
23	470.0
24	757.0
25	1149.0
26	1729.0
27	2369.0
28	3307.0
29	4482.0
30	5667.0
31	7153.0
32	9281.0
33	12108.0
34	16486.0
35	23700.0
36	36056.0
37	64523.0
38	131992.0
39	410662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97543646957956	19.33258517778251	12.00325474969555	24.68872360294238
2	18.8283993293905	21.351922541326037	35.38202351502045	24.437654614263014
3	19.457778360283314	22.79703686713959	30.47546104403196	27.269723728545138
4	13.721964645554483	16.17940988548305	35.855627822648906	34.24299764631356
5	14.494421599305362	36.16949818968201	34.81285189250588	14.523228318506748
6	34.17596400115773	35.78422538595542	15.508800384453655	14.531010228433189
7	28.808357498211528	30.47791848927189	21.73118825668835	18.98253575582824
8	25.989940857484562	34.35180786054817	20.47488764014264	19.183363641824624
9	25.249567216588304	14.298645128524386	20.74315874550151	39.7086289093858
10	16.27047188409596	27.100023482254514	34.757968948814145	21.87153568483538
11	34.337745812786366	22.540916463244812	22.085196895973613	21.03614082799522
12	20.45522807822321	25.496676987936674	30.85568243254313	23.192412501296985
13	30.072986123625878	20.774422909942825	25.158095643768736	23.99449532266256
14	21.810099553837166	21.898158008267938	26.327703053239187	29.96403938465571
15	23.69072778606028	28.81381848763359	23.857970587110973	23.637483139195158
16	24.028216932343803	27.126645805687073	25.124374034087495	23.720763227881626
17	22.44821616780528	27.068349743606547	26.697412037112883	23.786022051475285
18	22.54610440319577	26.07854541085754	27.809952107122772	23.56539807882392
19	24.29198272142947	26.097112774892555	27.711108198583418	21.899796305094558
20	24.227133472042464	26.277325425820653	27.21593298273781	22.27960811939907
21	23.044146638487963	26.39227925315509	27.16664755320369	23.396926555153264
22	23.880360643741433	25.78856687254597	27.217571279564435	23.113501204148168
23	22.494225003686168	26.20578646439162	27.443929291108965	23.856059240813252
24	23.212072063216414	26.524162147697922	26.97496682448926	23.288798964596406
25	23.34245318566818	26.334392765281212	27.13565643823348	23.18749761081713
26	23.61946187410235	26.452896235739992	26.531397958682152	23.396243931475507
27	23.412626899741696	26.247563033470406	26.633381936139187	23.70642813064871
28	23.152820327987026	26.41262143875227	27.269996778016242	23.164561455244463
29	23.24251707924442	26.632835837196982	27.659911422751577	22.464735660807026
30	23.426415898032403	26.145306006542263	26.801580410338744	23.626697685086583
31	22.892331132554595	25.86611292233927	27.574856512502937	23.6666994326032
32	22.42828355641475	26.428185258605154	27.442154469546793	23.7013767154333
33	22.865572284386484	25.778600566850702	27.623459318359302	23.73236783040351
34	23.30381668550708	25.47496955498397	28.0151487846568	23.206064974852143
35	23.13438948868756	25.58651026392962	27.460721833581808	23.81837841380101
36	22.71689684737081	25.287111518864986	27.47997182129458	24.516019812469622
37	22.31537760011359	24.85637597819973	28.976146398204428	23.852100023482254
38	22.229776590922743	24.54837617479535	28.745965694064452	24.475881540217458
39	21.699241468569276	25.010512404637474	29.803622820382596	23.486623306410657
40	22.228274818831675	24.370347919636078	29.523610587766292	23.87776667376595
41	21.21717262733662	24.704560472266365	29.870519940802875	24.20774695959414
42	20.689231474958632	25.25229771129933	29.794066088893985	24.26440472484805
43	21.10249184947329	24.38427344266234	29.709557277587557	24.803677430276817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	871.0
1	652.0
2	433.0
3	795.0
4	1157.0
5	1157.0
6	1318.5
7	1480.0
8	1331.0
9	1182.0
10	1506.5
11	1831.0
12	1831.0
13	2848.5
14	3866.0
15	4916.5
16	5967.0
17	6209.0
18	6451.0
19	6451.0
20	8104.5
21	9758.0
22	9879.0
23	10000.0
24	11790.5
25	13581.0
26	13581.0
27	15092.5
28	16604.0
29	18724.5
30	20845.0
31	23232.0
32	25619.0
33	25619.0
34	29657.5
35	33696.0
36	35805.5
37	37915.0
38	38399.0
39	38883.0
40	38883.0
41	39657.0
42	40431.0
43	42111.5
44	43792.0
45	43560.5
46	43329.0
47	43329.0
48	43214.5
49	43100.0
50	44540.0
51	45980.0
52	47984.0
53	49988.0
54	49988.0
55	49907.0
56	49826.0
57	45268.5
58	40711.0
59	39196.5
60	37682.0
61	37682.0
62	34890.0
63	32098.0
64	26584.0
65	21070.0
66	18599.5
67	16129.0
68	16129.0
69	14327.5
70	12526.0
71	12249.0
72	11972.0
73	10392.5
74	8813.0
75	8813.0
76	5546.0
77	2279.0
78	1765.5
79	1252.0
80	932.0
81	612.0
82	612.0
83	489.5
84	367.0
85	281.0
86	195.0
87	145.0
88	95.0
89	95.0
90	74.5
91	54.0
92	33.0
93	12.0
94	12.0
95	12.0
96	12.0
97	7.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	732468.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.02503162404966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.18267891314629	64.23385269036828
2	7.42887417239605	10.701297943958224
3	1.5689739563007237	3.390161964596098
4	0.5899587576058675	1.6996719269379061
5	0.29309574192015114	1.0555115040336591
6	0.18508093996196226	0.7998276332261501
7	0.12264634045016645	0.6183524584648039
8	0.09261823004906708	0.5336664758598051
9	0.06334722134510398	0.41063270586091094
>10	0.3909677218935201	5.281947029736891
>50	0.03468554676764634	1.778951078281389
>100	0.04192688581583762	6.349098542894051
>500	0.004383265333163811	2.028746653145027
>1k	7.623070144632716E-4	1.1182813926368427
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2802	0.3825423090155474	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2309	0.3152356143886149	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1814	0.24765587029057923	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1222	0.1668332268440396	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	998	0.13625168608048407	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	938	0.12806020194738882	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	920	0.12560275670746027	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	871	0.1189130446654325	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	766	0.10457794743251583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3652473555158723E-4	0.0
10	0.0	0.0	0.0	1.3652473555158723E-4	0.0
11	0.0	0.0	0.0	4.095742066547617E-4	0.0
12	0.0	0.0	0.0	5.460989422063489E-4	0.0
13	0.0	0.0	0.0	5.460989422063489E-4	0.0
14	0.0	0.0	0.0	5.460989422063489E-4	0.0
15	0.0	0.0	0.0	5.460989422063489E-4	0.0
16	0.0	0.0	0.0	5.460989422063489E-4	0.0
17	0.0	0.0	0.0	6.826236777579362E-4	0.0
18	0.0	0.0	0.0	8.191484133095234E-4	0.0
19	0.0	0.0	0.0	8.191484133095234E-4	0.0
20	0.0	0.0	0.0	0.0012287226199642852	0.0
21	0.0	0.0	0.0	0.001774821562170634	0.0
22	0.0	0.0	0.0	0.002320920504376983	0.0
23	0.0	0.0	0.0	0.0047783657443055535	0.0
24	0.0	0.0	0.0	0.011058503579678566	0.0
25	0.0	0.0	0.0	0.017611690886154753	0.0
26	0.0	0.0	0.0	0.03276593653238093	0.0
27	0.0	0.0	0.0	0.11085808526788883	0.0
28	0.0	0.0	0.0	0.26431188802787287	0.0
29	0.0	2.7304947110317446E-4	0.0	0.4139429981924125	0.0
30	0.0	2.7304947110317446E-4	0.0	0.5899233823184085	0.0
31	0.0	2.7304947110317446E-4	0.0	0.9530791788856305	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	610	0.0	28.508196	1
CCTTATA	80	6.9530324E-7	20.8125	2
GTACGAT	45	0.0038245039	20.555555	6
GTATCAA	920	0.0	18.902174	2
GTATAAC	60	9.232458E-4	18.5	1
ACCGTGT	60	9.232458E-4	18.5	8
AGTACGA	65	0.0015791944	17.076923	5
TAGGACA	90	4.442416E-5	16.444445	4
GTCTAAT	70	0.0025912898	15.857143	1
TCTATAG	70	0.0025912898	15.857143	3
AACTGTG	210	0.0	15.857142	7
GTTATAT	105	9.333769E-6	15.857142	1
CTCTTAG	110	1.4503594E-5	15.136364	1
GTATATA	135	3.9661245E-7	15.074075	1
GGATAGA	75	0.0041025155	14.8	1
GTTCTAG	90	8.268966E-4	14.388888	1
GTGTAAG	90	8.268966E-4	14.388888	1
GTATTAG	155	1.2078453E-7	14.322581	1
CTAGTCC	105	1.6543377E-4	14.095238	3
ACTGTCC	120	3.2971475E-5	13.874999	8
>>END_MODULE