FastQCFastQC Report
Fri 10 Feb 2017
ERR1630055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021552
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24720.24198474478049087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.22788854605541373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19920.19499741569690038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.13518646138424673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.027.050421
TACGCAA358.8687177E-426.4285720
CGTGCGA855.1793904E-821.76470610
AGAACCG555.1425566E-420.1818185
AACTGCG500.00703435918.57
TGTACGA500.00703435918.527
CTACACT1258.574716E-917.764
GAACCGC650.001579771617.0769236
ATCTCGC1757.2759576E-1216.91428611
TGCGAGA1107.805502E-716.81818212
CTTAGAC803.3821038E-416.18753
CGAGAAT1059.34098E-615.85714414
GTGCGAG1201.93489E-615.41666611
CGGACTG855.363427E-415.2352935
GTATCAA11400.014.9298261
GTACTAG750.0041039914.81
CTAGCAC2151.2732926E-1114.6279073
GTGCTAC1051.655346E-414.0952391
GTGTAAT1051.655346E-414.0952391
GTTCTAC3300.014.0151511
Produced by FastQC (version 0.11.5)