##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630055.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1021552
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87829204974392	34.0	31.0	34.0	31.0	34.0
2	33.098450201262395	34.0	33.0	34.0	31.0	34.0
3	33.22678825943271	34.0	33.0	34.0	31.0	34.0
4	36.516489615800275	37.0	37.0	37.0	35.0	37.0
5	36.460564905163906	37.0	37.0	37.0	35.0	37.0
6	36.49952131658496	37.0	37.0	37.0	35.0	37.0
7	36.430134736166146	37.0	37.0	37.0	35.0	37.0
8	36.435271038576595	37.0	37.0	37.0	35.0	37.0
9	38.21446191676978	39.0	39.0	39.0	37.0	39.0
10	38.19419863110248	39.0	39.0	39.0	37.0	39.0
11	38.23025357495262	39.0	39.0	39.0	37.0	39.0
12	38.16922290788917	39.0	39.0	39.0	37.0	39.0
13	38.1794475464783	39.0	39.0	39.0	37.0	39.0
14	39.644844315316305	41.0	40.0	41.0	37.0	41.0
15	39.637421296223785	41.0	40.0	41.0	37.0	41.0
16	39.55975124124861	41.0	40.0	41.0	37.0	41.0
17	39.4880622817047	41.0	39.0	41.0	37.0	41.0
18	39.39135354832647	41.0	39.0	41.0	36.0	41.0
19	39.421021152129306	41.0	39.0	41.0	37.0	41.0
20	39.63434656287688	41.0	40.0	41.0	37.0	41.0
21	39.64627742885335	41.0	40.0	41.0	37.0	41.0
22	39.65671253152067	41.0	40.0	41.0	37.0	41.0
23	39.59341962034238	41.0	40.0	41.0	37.0	41.0
24	39.56025733393895	41.0	40.0	41.0	37.0	41.0
25	39.540353305558604	41.0	40.0	41.0	37.0	41.0
26	39.40754068319576	41.0	40.0	41.0	37.0	41.0
27	39.33445776622238	41.0	39.0	41.0	36.0	41.0
28	39.29751299982771	41.0	39.0	41.0	36.0	41.0
29	39.1948789684715	41.0	39.0	41.0	36.0	41.0
30	39.11630342850878	41.0	39.0	41.0	36.0	41.0
31	38.99041654267233	40.0	39.0	41.0	35.0	41.0
32	38.90334706407506	40.0	39.0	41.0	35.0	41.0
33	38.83950596739079	40.0	39.0	41.0	35.0	41.0
34	38.77030635738563	40.0	39.0	41.0	35.0	41.0
35	38.648195099221574	40.0	38.0	41.0	35.0	41.0
36	38.64301376728742	40.0	38.0	41.0	35.0	41.0
37	38.605738131783795	40.0	38.0	41.0	35.0	41.0
38	38.51547351480884	40.0	38.0	41.0	35.0	41.0
39	38.47213161933998	40.0	38.0	41.0	35.0	41.0
40	38.415814368725236	40.0	38.0	41.0	35.0	41.0
41	38.321823069212336	40.0	38.0	41.0	34.0	41.0
42	38.242908828919134	40.0	38.0	41.0	34.0	41.0
43	37.95527197832318	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	7.0
17	16.0
18	33.0
19	55.0
20	97.0
21	166.0
22	356.0
23	597.0
24	972.0
25	1454.0
26	2068.0
27	2969.0
28	4069.0
29	5382.0
30	7210.0
31	9198.0
32	12061.0
33	15617.0
34	21743.0
35	30976.0
36	46619.0
37	82711.0
38	184340.0
39	592835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.785976631635	19.69278118000846	12.377343493038044	25.143898695318494
2	18.074361363885536	21.605557034786287	36.177404576565856	24.14267702476232
3	18.578104687769198	23.829036603129357	31.551110467210673	26.041748241890772
4	14.062916033642928	16.105983836358796	36.54840869578837	33.28269143420991
5	13.681535545914453	37.105208545428916	35.60102667314048	13.612229235516155
6	32.99009742039563	36.88975206352687	15.817207543032563	14.302942973044935
7	27.38646686610177	31.446465769730764	22.85884614782214	18.308221216345324
8	25.48250113552712	35.358748257553216	20.68460538474791	18.474145222171753
9	25.391267404889817	14.960569799677353	20.83398593512616	38.814176860306674
10	15.727931617773741	27.537119989976038	35.32174573589988	21.41320265635034
11	33.95617648440804	22.69487994737419	23.126673923598602	20.222269644619168
12	19.8713330305261	26.084428399141697	31.456254796623178	22.587983773709023
13	28.946935643021597	21.106806114617758	26.012087490406756	23.93417075195389
14	21.252368944507964	21.911170454367472	26.92843829780569	29.90802230331887
15	23.58812865130703	29.228468056447444	23.774315942800758	23.409087349444764
16	23.21183845756261	28.204242172694098	25.319709618306263	23.26420975143703
17	22.302339968988363	27.84429965386001	27.174436543612075	22.678923833539557
18	22.18849358622958	26.93176656694911	27.934162920732376	22.945576926088933
19	23.268321142731843	26.69017338324432	28.303307124845333	21.738198349178507
20	23.33146036618792	26.526207182796373	28.007580622425486	22.134751828590225
21	22.48353485676696	27.056282989020623	27.507557128760947	22.95262502545147
22	22.869320406597023	26.741663664698418	27.639806882077465	22.74920904662709
23	22.8428900339875	26.83064591915047	27.48974109981675	22.836722947045278
24	23.10611696712453	26.875773333124503	27.4376634767491	22.580446223001864
25	23.282123670650147	26.221670556173354	27.63354190486632	22.86266386831018
26	22.88576597177628	27.002639121650198	27.428755462277003	22.68283944429652
27	22.782491738061303	27.097788463044463	27.186966498034366	22.93275330085987
28	22.69859977759331	26.93480116528576	27.58303052610146	22.78356853101947
29	22.728456309615176	27.1429158770185	27.675928391310478	22.45269942205585
30	22.657290084107316	27.394983319498174	27.35847024918947	22.589256347205037
31	22.919929675630804	26.78532272463859	27.58626090497596	22.708486694754647
32	22.406495215123655	26.960350525474965	27.833042272933735	22.80011198646765
33	22.46229266841042	26.745187714379686	27.88991651917866	22.902603098031232
34	22.648675740441995	26.30262580857362	27.815617805065234	23.23308064591915
35	22.631251272573497	26.377022412955974	28.124951054865537	22.866775259604992
36	22.642802324306547	26.18006719188059	28.098227011449247	23.078903472363617
37	22.67148417310132	25.144877598007735	28.631435306279073	23.55220292261187
38	22.200631979576173	25.56042178959074	29.20174401303115	23.037202217801934
39	21.838144313750057	25.66712218271806	29.456356602502858	23.03837690102902
40	21.724004260184504	24.98727426503986	29.92339107553996	23.365330399235674
41	21.234846586370544	25.271939167071277	30.081092298776763	23.412121947781415
42	21.161037323601736	25.197836233495703	29.7670603160681	23.874066126834464
43	20.796102401052515	25.183837925039548	30.09636318072893	23.923696493179005
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	627.0
1	525.0
2	423.0
3	828.5
4	1234.0
5	1234.0
6	1388.5
7	1543.0
8	1361.0
9	1179.0
10	1617.0
11	2055.0
12	2055.0
13	3186.0
14	4317.0
15	5737.0
16	7157.0
17	7814.5
18	8472.0
19	8472.0
20	10656.5
21	12841.0
22	14169.0
23	15497.0
24	17982.5
25	20468.0
26	20468.0
27	22991.5
28	25515.0
29	30113.5
30	34712.0
31	37821.0
32	40930.0
33	40930.0
34	44213.0
35	47496.0
36	51379.0
37	55262.0
38	58235.5
39	61209.0
40	61209.0
41	64366.0
42	67523.0
43	66422.0
44	65321.0
45	66121.5
46	66922.0
47	66922.0
48	67142.0
49	67362.0
50	68303.5
51	69245.0
52	69397.0
53	69549.0
54	69549.0
55	64088.5
56	58628.0
57	54709.5
58	50791.0
59	46927.0
60	43063.0
61	43063.0
62	39240.0
63	35417.0
64	31408.0
65	27399.0
66	23915.5
67	20432.0
68	20432.0
69	17267.0
70	14102.0
71	11983.5
72	9865.0
73	8118.0
74	6371.0
75	6371.0
76	5097.5
77	3824.0
78	3090.0
79	2356.0
80	1777.5
81	1199.0
82	1199.0
83	926.0
84	653.0
85	485.0
86	317.0
87	234.0
88	151.0
89	151.0
90	109.0
91	67.0
92	52.0
93	37.0
94	23.5
95	10.0
96	10.0
97	7.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1021552.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.45318607126892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.24839892069065	59.724308857383356
2	7.9444605286819945	10.876472696114401
3	2.058265008614069	4.226843928559224
4	0.8562033118617847	2.3443937848684575
5	0.45830525920850923	1.5686227593020612
6	0.28692024020591245	1.1784362754257098
7	0.18645928628507827	0.8934612553151927
8	0.1279854500102927	0.7008809459175723
9	0.10522940510448162	0.6482959243007415
>10	0.6126422930715136	8.044054812685422
>50	0.07259554844415236	3.520729842380525
>100	0.040954065184088	5.053070120649874
>500	0.0010058889510866152	0.4164822702434737
>1k	5.747936863352087E-4	0.8039465268540176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2472	0.24198474478049087	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2328	0.22788854605541373	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1992	0.19499741569690038	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1381	0.13518646138424673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.789026892414679E-5	0.0
5	0.0	0.0	0.0	9.789026892414679E-5	0.0
6	0.0	0.0	0.0	9.789026892414679E-5	0.0
7	0.0	0.0	0.0	1.9578053784829357E-4	0.0
8	0.0	0.0	0.0	1.9578053784829357E-4	0.0
9	0.0	0.0	0.0	2.9367080677244036E-4	0.0
10	0.0	0.0	0.0	3.9156107569658714E-4	0.0
11	0.0	0.0	0.0	3.9156107569658714E-4	0.0
12	0.0	0.0	0.0	4.89451344620734E-4	0.0
13	0.0	0.0	0.0	4.89451344620734E-4	0.0
14	0.0	0.0	0.0	4.89451344620734E-4	0.0
15	0.0	0.0	0.0	5.873416135448807E-4	0.0
16	0.0	0.0	0.0	6.852318824690276E-4	0.0
17	0.0	0.0	0.0	6.852318824690276E-4	0.0
18	0.0	0.0	0.0	6.852318824690276E-4	0.0
19	0.0	0.0	0.0	7.831221513931743E-4	0.0
20	0.0	0.0	0.0	0.0010767929581656146	0.0
21	0.0	0.0	0.0	0.0011746832270897614	0.0
22	0.0	0.0	0.0	0.0016641345717104954	0.0
23	0.0	0.0	0.0	0.003328269143420991	0.0
24	0.0	0.0	0.0	0.007831221513931744	0.0
25	0.0	0.0	0.0	0.01282362522906323	0.0
26	0.0	0.0	0.0	0.023004213197174495	0.0
27	0.0	0.0	0.0	0.08477297288831112	0.0
28	0.0	0.0	0.0	0.19441007408335553	0.0
29	0.0	0.0	0.0	0.3231357777186086	0.0
30	0.0	0.0	0.0	0.4671323633060285	0.0
31	0.0	0.0	0.0	0.7672639278274626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	595	0.0	27.05042	1
TACGCAA	35	8.8687177E-4	26.42857	20
CGTGCGA	85	5.1793904E-8	21.764706	10
AGAACCG	55	5.1425566E-4	20.181818	5
AACTGCG	50	0.007034359	18.5	7
TGTACGA	50	0.007034359	18.5	27
CTACACT	125	8.574716E-9	17.76	4
GAACCGC	65	0.0015797716	17.076923	6
ATCTCGC	175	7.2759576E-12	16.914286	11
TGCGAGA	110	7.805502E-7	16.818182	12
CTTAGAC	80	3.3821038E-4	16.1875	3
CGAGAAT	105	9.34098E-6	15.857144	14
GTGCGAG	120	1.93489E-6	15.416666	11
CGGACTG	85	5.363427E-4	15.235293	5
GTATCAA	1140	0.0	14.929826	1
GTACTAG	75	0.00410399	14.8	1
CTAGCAC	215	1.2732926E-11	14.627907	3
GTGCTAC	105	1.655346E-4	14.095239	1
GTGTAAT	105	1.655346E-4	14.095239	1
GTTCTAC	330	0.0	14.015151	1
>>END_MODULE