FastQCFastQC Report
Fri 10 Feb 2017
ERR1630054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665093
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19040.2862757539171214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.2455295725560185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13040.19606280625416297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.1407321983542151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCGA303.5978624E-430.83333224
GGTATCA3550.029.7042261
TGCGTTA502.7004434E-422.237
CTTGTCG450.003824137620.55555723
ATGCGTT555.1399623E-420.18181836
CCTATAC951.673452E-719.4736843
GCCTAGC500.00703184118.51
ACTTAAG650.00157898817.0769231
CTTACCC650.00157898817.0769233
TTACCCC904.4414242E-516.4444454
CTTATAC5500.016.14545437
CTACAGT1059.331177E-615.8571444
AATGCGT700.002590953415.85714335
GTATCAA6650.015.8571422
AGCGCTC855.3599314E-415.23529436
GTGTTAG1101.4499607E-515.1363641
AATCTCG1508.082134E-814.810
CACACGG750.00410198814.830
GTCTTAG1001.09226356E-414.81
GCTTAGG2151.2732926E-1114.6279071
Produced by FastQC (version 0.11.5)