##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630054.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665093
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88344938226684	34.0	31.0	34.0	31.0	34.0
2	33.104499671474514	34.0	33.0	34.0	31.0	34.0
3	33.23048957063148	34.0	33.0	34.0	31.0	34.0
4	36.50927013214693	37.0	37.0	37.0	35.0	37.0
5	36.452799834008175	37.0	37.0	37.0	35.0	37.0
6	36.49580284260998	37.0	37.0	37.0	35.0	37.0
7	36.43375137010914	37.0	37.0	37.0	35.0	37.0
8	36.44000613448044	37.0	37.0	37.0	35.0	37.0
9	38.21763121849125	39.0	39.0	39.0	37.0	39.0
10	38.19881279760875	39.0	39.0	39.0	37.0	39.0
11	38.22976185285366	39.0	39.0	39.0	37.0	39.0
12	38.173962137625864	39.0	39.0	39.0	37.0	39.0
13	38.18700392275967	39.0	39.0	39.0	37.0	39.0
14	39.656857010974406	41.0	40.0	41.0	37.0	41.0
15	39.6458495278104	41.0	40.0	41.0	37.0	41.0
16	39.563757248986235	41.0	40.0	41.0	37.0	41.0
17	39.49531418914347	41.0	39.0	41.0	37.0	41.0
18	39.393436707347696	41.0	39.0	41.0	36.0	41.0
19	39.41903312769793	41.0	39.0	41.0	37.0	41.0
20	39.63889260599645	41.0	40.0	41.0	37.0	41.0
21	39.65057217562055	41.0	40.0	41.0	37.0	41.0
22	39.66943119232949	41.0	40.0	41.0	37.0	41.0
23	39.60869081466802	41.0	40.0	41.0	37.0	41.0
24	39.576363004873	41.0	40.0	41.0	37.0	41.0
25	39.55457808156152	41.0	40.0	41.0	37.0	41.0
26	39.41884668760609	41.0	40.0	41.0	37.0	41.0
27	39.34148607788685	41.0	39.0	41.0	36.0	41.0
28	39.3025546803229	41.0	39.0	41.0	36.0	41.0
29	39.20325127463377	41.0	39.0	41.0	36.0	41.0
30	39.130410333592444	41.0	39.0	41.0	36.0	41.0
31	39.007400468806615	40.0	39.0	41.0	35.0	41.0
32	38.92277771680051	40.0	39.0	41.0	35.0	41.0
33	38.85447599057576	40.0	39.0	41.0	35.0	41.0
34	38.786434378350094	40.0	39.0	41.0	35.0	41.0
35	38.66117971471659	40.0	38.0	41.0	35.0	41.0
36	38.649137789752714	40.0	38.0	41.0	35.0	41.0
37	38.60120765065938	40.0	38.0	41.0	35.0	41.0
38	38.50618484933686	40.0	38.0	41.0	35.0	41.0
39	38.472843647429755	40.0	38.0	41.0	35.0	41.0
40	38.420102151127736	40.0	38.0	41.0	35.0	41.0
41	38.330050083221444	40.0	38.0	41.0	35.0	41.0
42	38.230805315948295	40.0	38.0	41.0	34.0	41.0
43	37.949413089597996	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	1.0
16	2.0
17	6.0
18	17.0
19	23.0
20	61.0
21	124.0
22	222.0
23	386.0
24	603.0
25	979.0
26	1324.0
27	1945.0
28	2592.0
29	3499.0
30	4582.0
31	5966.0
32	7796.0
33	10236.0
34	14313.0
35	20216.0
36	30484.0
37	53895.0
38	118190.0
39	387627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.902872229898676	19.639809770964362	12.417060471242367	25.040257527894592
2	18.484332266314635	21.524508602556335	35.69335416250058	24.297804968628448
3	19.049215673597526	23.520470069599288	31.276227535096595	26.154086721706587
4	14.450761021390992	16.035952866741944	35.82175725800752	33.69152885385954
5	13.98451043688627	36.9542304610032	35.23822984154096	13.823029260569575
6	33.541625005826255	35.98519304819025	15.638414477373841	14.834767468609652
7	27.7410828260108	31.064076753175872	22.50572476330378	18.68911565750955
8	25.718809249232816	34.996308786891454	20.439998616734805	18.844883347140925
9	25.4653108662999	15.046918250530378	20.727627564866868	38.76014331830285
10	16.21833337593389	27.53614907990311	34.81257508348457	21.43294246067843
11	34.129212004937656	22.574888023178712	22.701035794994084	20.594864176889548
12	20.166503030403266	25.915022410399747	31.01866956951885	22.899804989678135
13	29.251999344452578	20.92534427516152	25.691745364933926	24.130911015451975
14	21.579237790805195	21.959635720117337	26.30729837782085	30.15382811125662
15	23.85004803839463	28.94151644957923	23.61083337217502	23.59760213985112
16	23.370716576478777	27.873395149249802	25.18068901642327	23.57519925784815
17	22.54361420132222	27.828439030331097	26.726788584453605	22.90115818389308
18	22.480765847783694	26.82271501880188	27.52261713775367	23.173901995660756
19	23.535054496138134	26.47253842695683	28.161475162120187	21.83093191478485
20	23.635190868044017	26.303088440263238	27.637638646023937	22.4240820456688
21	22.75982456588778	26.945254272710734	27.04854809778482	23.24637306361667
22	23.090003954334207	26.507420766719843	27.172139836083076	23.23043544286288
23	22.99061935699218	26.794598650113592	27.06027578098101	23.15450621191322
24	23.423491150861608	26.741673720817992	27.0163721464517	22.818462981868702
25	23.605721305140783	26.143110813074262	27.292574121213125	22.95859376057183
26	23.14142533450209	26.627103277286036	27.221155537646613	23.01031585056526
27	23.05076132210082	26.615826658828162	27.07395807804322	23.259453941027797
28	22.806885653585287	26.721826872332137	27.373615419197016	23.097672054885557
29	22.999490296845703	26.92044571210342	27.406392790181222	22.673671200869652
30	22.744187654959532	27.38985374977635	27.028250184560655	22.837708410703467
31	23.09616850575784	26.53448465101873	27.416767279162464	22.952579564060965
32	22.55564259434395	26.824519277755144	27.574188872834327	23.045649255066586
33	22.686150658629696	26.460660388847874	27.58125555373459	23.27193339878784
34	22.722536547520424	26.043124796081152	27.661545077154624	23.572793579243807
35	22.714417382230756	26.035907760268113	27.875349763115835	23.374325094385295
36	22.565265308761333	25.901340113337533	28.14478576680254	23.3886088110986
37	22.780272834024714	24.951999194097667	28.447450206211762	23.820277765665853
38	22.262300159526564	25.47448251597897	29.067513866481832	23.19570345801264
39	21.939488161805944	25.214518871796876	29.47888490782492	23.36710805857226
40	21.70779124122491	24.835925201437995	29.92619077332042	23.53009278401667
41	21.27446838261717	25.02552274644298	29.923935449628846	23.776073421311004
42	21.096297810982826	24.85727559905156	29.93626455247612	24.110162037489495
43	20.731837502424472	25.02837948978564	29.955209271485337	24.284573736304544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	583.0
1	458.5
2	334.0
3	612.0
4	890.0
5	890.0
6	976.0
7	1062.0
8	932.5
9	803.0
10	1122.0
11	1441.0
12	1441.0
13	2254.5
14	3068.0
15	3951.0
16	4834.0
17	5184.0
18	5534.0
19	5534.0
20	6744.0
21	7954.0
22	8572.5
23	9191.0
24	10667.5
25	12144.0
26	12144.0
27	13651.0
28	15158.0
29	18076.0
30	20994.0
31	22986.5
32	24979.0
33	24979.0
34	26957.0
35	28935.0
36	31471.5
37	34008.0
38	35907.0
39	37806.0
40	37806.0
41	40375.0
42	42944.0
43	42247.0
44	41550.0
45	42581.0
46	43612.0
47	43612.0
48	44142.5
49	44673.0
50	45970.5
51	47268.0
52	47570.5
53	47873.0
54	47873.0
55	44157.5
56	40442.0
57	37839.0
58	35236.0
59	32025.0
60	28814.0
61	28814.0
62	26354.0
63	23894.0
64	21306.0
65	18718.0
66	16448.5
67	14179.0
68	14179.0
69	11878.5
70	9578.0
71	8119.0
72	6660.0
73	5498.5
74	4337.0
75	4337.0
76	3395.0
77	2453.0
78	2032.0
79	1611.0
80	1217.0
81	823.0
82	823.0
83	594.0
84	365.0
85	271.5
86	178.0
87	138.0
88	98.0
89	98.0
90	69.0
91	40.0
92	30.0
93	20.0
94	12.5
95	5.0
96	5.0
97	3.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	665093.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.87055237956599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.45775618436114	61.107217344787166
2	7.723654204272419	10.793119712825423
3	2.0167615933444485	4.227367396346108
4	0.8599113700779003	2.4032992969924916
5	0.46633185402108573	1.62914321163202
6	0.30750755376046424	1.2891433585279632
7	0.20372721814132275	0.996417328640059
8	0.12760722199002048	0.7132789670451705
9	0.10819877539457773	0.6803917383250565
>10	0.6303457403087401	8.239464143615633
>50	0.06770069708133429	3.273111702563343
>100	0.029416112521677796	3.6989715493018225
>500	4.325898899813135E-4	0.21747099440819836
>1k	6.488848349719703E-4	0.7316032549896374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1904	0.2862757539171214	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1633	0.2455295725560185	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1304	0.19606280625416297	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.1407321983542151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.007098255431947E-4	0.0	0.0	0.0
6	0.0	3.007098255431947E-4	0.0	0.0	0.0
7	0.0	3.007098255431947E-4	0.0	0.0	0.0
8	0.0	3.007098255431947E-4	0.0	0.0	0.0
9	0.0	3.007098255431947E-4	0.0	1.5035491277159735E-4	0.0
10	0.0	3.007098255431947E-4	0.0	1.5035491277159735E-4	0.0
11	0.0	3.007098255431947E-4	0.0	1.5035491277159735E-4	0.0
12	0.0	3.007098255431947E-4	0.0	1.5035491277159735E-4	0.0
13	0.0	3.007098255431947E-4	0.0	1.5035491277159735E-4	0.0
14	0.0	3.007098255431947E-4	0.0	3.007098255431947E-4	0.0
15	0.0	3.007098255431947E-4	0.0	4.5106473831479205E-4	0.0
16	0.0	3.007098255431947E-4	0.0	6.014196510863894E-4	0.0
17	0.0	3.007098255431947E-4	0.0	6.014196510863894E-4	0.0
18	0.0	3.007098255431947E-4	0.0	6.014196510863894E-4	0.0
19	0.0	3.007098255431947E-4	0.0	7.517745638579868E-4	0.0
20	0.0	3.007098255431947E-4	0.0	7.517745638579868E-4	0.0
21	0.0	3.007098255431947E-4	0.0	7.517745638579868E-4	0.0
22	0.0	3.007098255431947E-4	0.0	0.0012028393021727788	0.0
23	0.0	3.007098255431947E-4	0.0	0.0036085179065183364	0.0
24	0.0	3.007098255431947E-4	0.0	0.009021294766295842	0.0
25	0.0	3.007098255431947E-4	0.0	0.017741879707048488	0.0
26	0.0	3.007098255431947E-4	0.0	0.029469562903233083	0.0
27	0.0	3.007098255431947E-4	0.0	0.10675198806783412	0.0
28	0.0	4.5106473831479205E-4	0.0	0.23756076217912384	0.0
29	0.0	4.5106473831479205E-4	0.0	0.3867128356485484	0.0
30	0.0	4.5106473831479205E-4	0.0	0.5507500454823611	0.0
31	0.0	4.5106473831479205E-4	0.0	0.8744641726796102	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCGA	30	3.5978624E-4	30.833332	24
GGTATCA	355	0.0	29.704226	1
TGCGTTA	50	2.7004434E-4	22.2	37
CTTGTCG	45	0.0038241376	20.555557	23
ATGCGTT	55	5.1399623E-4	20.181818	36
CCTATAC	95	1.673452E-7	19.473684	3
GCCTAGC	50	0.007031841	18.5	1
ACTTAAG	65	0.001578988	17.076923	1
CTTACCC	65	0.001578988	17.076923	3
TTACCCC	90	4.4414242E-5	16.444445	4
CTTATAC	550	0.0	16.145454	37
CTACAGT	105	9.331177E-6	15.857144	4
AATGCGT	70	0.0025909534	15.857143	35
GTATCAA	665	0.0	15.857142	2
AGCGCTC	85	5.3599314E-4	15.235294	36
GTGTTAG	110	1.4499607E-5	15.136364	1
AATCTCG	150	8.082134E-8	14.8	10
CACACGG	75	0.004101988	14.8	30
GTCTTAG	100	1.09226356E-4	14.8	1
GCTTAGG	215	1.2732926E-11	14.627907	1
>>END_MODULE