FastQCFastQC Report
Fri 10 Feb 2017
ERR1630053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394202
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11710.2970558241713639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.28843080451139264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8340.21156665871811914No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC7850.19913648332580755No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.148908427658916No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT4350.1103495162378679No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG4070.10324655887083273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3250.027.3230761
TAGCACT400.00192894923.1254
AAACGTA450.003821416820.55555514
GTAAACG450.003821416820.55555512
GAGGCGT450.003821416820.55555522
GTAATAG656.887437E-519.9230771
TAAACGT500.00702688518.513
GACTAGG609.222147E-418.51
GTATCAA4900.018.122452
TGCATCA2350.018.10638214
GCCTAAG953.5942412E-617.5263141
ATACACA2050.017.14634337
AACGTAG650.001577445517.07692315
GCATCAG2600.017.07692315
GCGGGTG1504.638423E-916.03333313
ATAGGCA700.002588444215.85714224
ACGTAGG700.002588444215.85714216
GGACACT700.002588444215.8571426
ATCAAGC2750.015.47272730
TATACAC2300.015.28260736
Produced by FastQC (version 0.11.5)