FastQCFastQC Report
Fri 10 Feb 2017
ERR1630052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961130
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55300.5753644148034085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48550.505134581170081No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37980.395159863910189No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28490.2964219200316294No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC18440.19185750106645302No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT18250.18988066130492232No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.16636667256250456No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA15740.16376556761312205No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA14990.15596225276497455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.15232070583583907No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13510.14056371146463018No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG13360.13900304849500067No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA11020.1146567061687805No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.1022754466097198No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG9690.10081882783806562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.031.4645651
GCGACCA250.005495900329.612
GTACGGA250.005495900329.66
TAGTACG450.00382535920.5555554
AACCACG2350.020.4680847
GTATCAA19700.020.1903552
TTAGGCA902.1514697E-618.54
CGCGAAT500.00703406318.528
CATCGTT2300.018.532
ACCACGA2550.018.1372558
CGTGCGA1750.017.97142810
CCACGAC2500.017.7600029
CGACCAA2400.017.72916812
TCTATAC1156.4015694E-817.6956523
CTTAGAC953.6051424E-617.5263163
TACGGCT852.7223647E-517.41176431
ATCGTTG2450.017.36734633
CCATCGT2450.017.36734631
CCGCGAA752.0668223E-417.26666627
CGTTGTA2150.017.20930335
Produced by FastQC (version 0.11.5)