##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961130
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85977547262077	34.0	31.0	34.0	31.0	34.0
2	33.074622579671846	34.0	33.0	34.0	31.0	34.0
3	33.19846950984778	34.0	33.0	34.0	31.0	34.0
4	36.47855857168125	37.0	37.0	37.0	35.0	37.0
5	36.435816174711015	37.0	37.0	37.0	35.0	37.0
6	36.4908222612966	37.0	37.0	37.0	35.0	37.0
7	36.41808080072415	37.0	37.0	37.0	35.0	37.0
8	36.42529834673769	37.0	37.0	37.0	35.0	37.0
9	38.19571650036936	39.0	39.0	39.0	37.0	39.0
10	38.18890472672791	39.0	39.0	39.0	37.0	39.0
11	38.21577310041305	39.0	39.0	39.0	37.0	39.0
12	38.16078053957321	39.0	39.0	39.0	37.0	39.0
13	38.17637364352377	39.0	39.0	39.0	37.0	39.0
14	39.61393984164473	41.0	40.0	41.0	37.0	41.0
15	39.614975081414585	41.0	40.0	41.0	37.0	41.0
16	39.51491161445382	41.0	39.0	41.0	37.0	41.0
17	39.483679627105595	41.0	39.0	41.0	37.0	41.0
18	39.38702672895446	41.0	39.0	41.0	36.0	41.0
19	39.386466971169355	41.0	39.0	41.0	36.0	41.0
20	39.62053104158647	41.0	40.0	41.0	37.0	41.0
21	39.61770624161144	41.0	40.0	41.0	37.0	41.0
22	39.62783182295839	41.0	40.0	41.0	37.0	41.0
23	39.56330465181609	41.0	40.0	41.0	37.0	41.0
24	39.532294278609555	41.0	40.0	41.0	37.0	41.0
25	39.50792088479186	41.0	40.0	41.0	37.0	41.0
26	39.34875823249717	41.0	39.0	41.0	36.0	41.0
27	39.2619884927117	41.0	39.0	41.0	36.0	41.0
28	39.19910417945543	41.0	39.0	41.0	35.0	41.0
29	39.105377004151364	41.0	39.0	41.0	35.0	41.0
30	39.008140418049585	41.0	39.0	41.0	35.0	41.0
31	38.85171204727769	40.0	39.0	41.0	35.0	41.0
32	38.78847190286434	40.0	38.0	41.0	35.0	41.0
33	38.692717946583706	40.0	38.0	41.0	35.0	41.0
34	38.6226514623412	40.0	38.0	41.0	35.0	41.0
35	38.48243525849781	40.0	38.0	41.0	35.0	41.0
36	38.451424885291274	40.0	38.0	41.0	35.0	41.0
37	38.394473172203554	40.0	38.0	41.0	35.0	41.0
38	38.29949434519784	40.0	38.0	41.0	35.0	41.0
39	38.23518774775525	40.0	38.0	41.0	34.0	41.0
40	38.15690489319863	40.0	38.0	41.0	34.0	41.0
41	38.049868384089564	40.0	38.0	41.0	34.0	41.0
42	37.95378772902729	40.0	38.0	41.0	34.0	41.0
43	37.65605901386909	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	1.0
16	7.0
17	11.0
18	24.0
19	38.0
20	81.0
21	162.0
22	314.0
23	523.0
24	900.0
25	1424.0
26	2060.0
27	2967.0
28	4067.0
29	5660.0
30	7444.0
31	9348.0
32	12022.0
33	15999.0
34	21978.0
35	30997.0
36	48415.0
37	86230.0
38	175036.0
39	535416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1558373997274	19.003464671792578	12.42995224371313	24.410745684766887
2	19.081393776076077	21.725677067618324	33.833820606993854	25.35910854931175
3	19.75206267622486	22.930716968568248	30.912363572045404	26.404856783161485
4	14.198495520897279	15.570630403795532	35.95122407998918	34.27964999531801
5	14.12649693589837	36.686192294486695	35.05738037518338	14.129930394431556
6	33.894894551205354	35.74157502106895	14.908180995286799	15.4553494324389
7	28.335188788197225	31.028268808589885	21.766878570016544	18.86966383319634
8	27.501898806613053	32.63980939102931	20.412535244972062	19.445756557385575
9	25.60059513281242	14.693225682269828	20.79645833550092	38.909720849416836
10	17.2245169748109	27.277475471580324	33.647373404222115	21.85063414938666
11	33.6785866636147	22.78172567706762	22.35483233277496	21.184855326542714
12	22.027197153350745	26.16919667474743	29.625024710497016	22.178581461404807
13	30.813417539770892	20.455505498735864	24.860424708416133	23.870652253077107
14	21.530906329008562	21.973614391393465	26.82758835953513	29.667890920062845
15	24.376931320424916	28.98380031837525	23.273022379906987	23.366245981292852
16	22.71794658370876	27.60063675049161	25.607566094076763	24.07385057172287
17	23.073777740784283	27.12265770499308	26.558321975175055	23.245242579047577
18	22.649381457243038	25.946021870090412	28.07206101151769	23.332535661148857
19	24.582730744020058	25.306358140938272	28.73575895040213	21.375152164639537
20	24.756172422044884	25.632952878382735	26.998637021006523	22.612237678565855
21	23.093754226795543	25.908357870423355	27.963334824633506	23.034553078147596
22	23.33357610312861	26.059846222675393	28.255490932548145	22.35108674164785
23	23.36811877685641	25.8812023347518	27.233464775836776	23.517214112555013
24	22.75914808610698	26.11238854265292	28.446516080030797	22.681947291209305
25	23.334720589306336	26.432844672416845	27.39275644293696	22.83967829533986
26	23.042148304599795	26.514935544619355	27.603237855440995	22.83967829533986
27	22.83593270421275	26.00033294143352	27.87978733365934	23.28394702069439
28	22.700259070052958	26.542715345478758	27.402744685942587	23.354280898525694
29	23.00812585186187	26.764121398770197	27.99402786303622	22.233724886331714
30	23.638737735790162	26.335979524101838	26.95358588328322	23.07169685682478
31	22.18003808017646	26.994579297285487	28.332171506455943	22.49321111608211
32	22.222175980356457	26.21830553619177	28.233329518379406	23.32618896507236
33	21.688013067951264	26.89573730920895	28.229583927252293	23.186665695587486
34	23.433978754174774	25.00244503865242	28.092245585924903	23.47133062124791
35	22.1596454173733	25.46117590752552	28.34174357266967	24.037435102431512
36	22.050086876905308	25.950807903197276	28.949569777241376	23.04953544265604
37	22.17025792556678	24.492732512771425	29.814593239208016	23.52241632245378
38	22.389479050700736	25.0914028279213	29.340775961628502	23.178342159749462
39	21.762196581107656	24.766368753446464	30.02361803294039	23.44781663250549
40	20.729661960400776	24.228876426706066	30.71561599367411	24.325845619219045
41	20.084067711964043	24.62788592594134	30.95231654406792	24.335729818026696
42	20.099986474254266	24.546107186332755	31.319176386128827	24.034729953284156
43	20.475586028945095	24.635064975601637	31.17393068575531	23.71541830969796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2313.0
1	1698.5
2	1084.0
3	1817.0
4	2550.0
5	2550.0
6	2753.0
7	2956.0
8	2416.5
9	1877.0
10	2714.0
11	3551.0
12	3551.0
13	5485.0
14	7419.0
15	9376.0
16	11333.0
17	11355.0
18	11377.0
19	11377.0
20	13614.0
21	15851.0
22	14886.0
23	13921.0
24	16623.0
25	19325.0
26	19325.0
27	21731.0
28	24137.0
29	26283.5
30	28430.0
31	31231.0
32	34032.0
33	34032.0
34	36464.5
35	38897.0
36	42522.5
37	46148.0
38	46290.0
39	46432.0
40	46432.0
41	48027.5
42	49623.0
43	50093.0
44	50563.0
45	51575.0
46	52587.0
47	52587.0
48	54928.0
49	57269.0
50	58301.5
51	59334.0
52	59449.5
53	59565.0
54	59565.0
55	57956.5
56	56348.0
57	55338.5
58	54329.0
59	53498.5
60	52668.0
61	52668.0
62	49219.5
63	45771.0
64	42229.0
65	38687.0
66	33085.5
67	27484.0
68	27484.0
69	24613.0
70	21742.0
71	17952.0
72	14162.0
73	9468.5
74	4775.0
75	4775.0
76	3517.0
77	2259.0
78	1715.5
79	1172.0
80	891.5
81	611.0
82	611.0
83	439.5
84	268.0
85	207.0
86	146.0
87	103.0
88	60.0
89	60.0
90	45.5
91	31.0
92	24.5
93	18.0
94	13.5
95	9.0
96	9.0
97	8.5
98	8.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	961130.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.4008694778374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.62468801140977	52.574302926691196
2	9.294212578388828	11.41345466849854
3	2.220795422315644	4.090763095877448
4	0.8534986820918659	2.096222447145155
5	0.41320256249010995	1.2685498303681597
6	0.2607390587945375	0.9605762950090542
7	0.2019170581970639	0.867851805499477
8	0.14594912869738952	0.7169122721242004
9	0.11402213986934547	0.6300952674931259
>10	0.7318307062700857	8.490429798408702
>50	0.0687236594180114	2.9063950636094367
>100	0.06070187228077613	7.604847031283139
>500	0.007502478423934806	3.1732055663354712
>1k	0.0020461304792549467	2.6274297038092644
>5k	1.705108732712456E-4	0.5789642278476742
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5530	0.5753644148034085	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4855	0.505134581170081	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3798	0.395159863910189	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2849	0.2964219200316294	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1844	0.19185750106645302	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1825	0.18988066130492232	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1599	0.16636667256250456	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1574	0.16376556761312205	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1499	0.15596225276497455	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1464	0.15232070583583907	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1351	0.14056371146463018	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1336	0.13900304849500067	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1102	0.1146567061687805	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	983	0.1022754466097198	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	969	0.10081882783806562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.0404419797529991E-4	1.0404419797529991E-4
11	0.0	0.0	0.0	2.0808839595059983E-4	1.0404419797529991E-4
12	0.0	0.0	0.0	4.1617679190119965E-4	1.0404419797529991E-4
13	0.0	0.0	0.0	4.1617679190119965E-4	1.0404419797529991E-4
14	0.0	0.0	0.0	4.1617679190119965E-4	1.0404419797529991E-4
15	0.0	0.0	0.0	4.1617679190119965E-4	3.1213259392589974E-4
16	0.0	0.0	0.0	4.1617679190119965E-4	3.1213259392589974E-4
17	0.0	0.0	0.0	6.242651878517995E-4	3.1213259392589974E-4
18	0.0	0.0	0.0	6.242651878517995E-4	3.1213259392589974E-4
19	0.0	0.0	0.0	7.283093858270993E-4	3.1213259392589974E-4
20	0.0	0.0	0.0	0.001248530375703599	3.1213259392589974E-4
21	0.0	0.0	0.0	0.002393016553431898	3.1213259392589974E-4
22	0.0	0.0	0.0	0.004161767919011996	3.1213259392589974E-4
23	0.0	0.0	0.0	0.0066588286704191944	3.1213259392589974E-4
24	0.0	0.0	0.0	0.01258934795501129	3.1213259392589974E-4
25	0.0	0.0	0.0	0.02236950256468948	3.1213259392589974E-4
26	0.0	0.0	0.0	0.037872088063009166	3.1213259392589974E-4
27	0.0	0.0	0.0	0.13244826402255677	3.1213259392589974E-4
28	0.0	0.0	0.0	0.29777449460530836	3.1213259392589974E-4
29	0.0	0.0	0.0	0.5050305369721058	3.1213259392589974E-4
30	0.0	0.0	0.0	0.7132229771206808	4.1617679190119965E-4
31	0.0	0.0	0.0	1.0980824654313153	4.1617679190119965E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1270	0.0	31.464565	1
GCGACCA	25	0.0054959003	29.6	12
GTACGGA	25	0.0054959003	29.6	6
TAGTACG	45	0.003825359	20.555555	4
AACCACG	235	0.0	20.468084	7
GTATCAA	1970	0.0	20.190355	2
TTAGGCA	90	2.1514697E-6	18.5	4
CGCGAAT	50	0.007034063	18.5	28
CATCGTT	230	0.0	18.5	32
ACCACGA	255	0.0	18.137255	8
CGTGCGA	175	0.0	17.971428	10
CCACGAC	250	0.0	17.760002	9
CGACCAA	240	0.0	17.729168	12
TCTATAC	115	6.4015694E-8	17.695652	3
CTTAGAC	95	3.6051424E-6	17.526316	3
TACGGCT	85	2.7223647E-5	17.411764	31
ATCGTTG	245	0.0	17.367346	33
CCATCGT	245	0.0	17.367346	31
CCGCGAA	75	2.0668223E-4	17.266666	27
CGTTGTA	215	0.0	17.209303	35
>>END_MODULE