FastQCFastQC Report
Fri 10 Feb 2017
ERR1630051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117718
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6820.5793506515571111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.5513175555140251No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4690.39840975891537406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.26504018077099506No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.11892828624339524No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATT1210.10278801882464873No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAG1190.10108904330688594No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.10023955554800455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT200.001835232737.034
CATCGTT200.001835232737.032
CTAATAA251.2269052E-437.024
ACGACCA303.580055E-430.83333211
GTCGATG250.005476569329.621
GGTCGAT250.005476569329.620
CATGCGG250.005476569329.626
AACCACG250.005476569329.67
CGACCAA250.005476569329.612
ACCACGA250.005476569329.68
CTCTAGC250.005476569329.62
GGTATCA2100.029.0714281
TCGATGA358.8219834E-426.42857222
AAAACCA358.8219834E-426.42857227
GAAAAAC358.8219834E-426.42857225
TGGATGG358.8219834E-426.4285725
TATTAAC400.001920962223.1252
TTAACCC400.001920962223.12516
ATAAAAC400.001920962223.12527
TGAGCCG400.001920962223.12510
Produced by FastQC (version 0.11.5)