##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630051.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117718
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74181518544318	34.0	31.0	34.0	31.0	34.0
2	32.9796462732972	34.0	31.0	34.0	31.0	34.0
3	33.101913046433	34.0	33.0	34.0	31.0	34.0
4	36.40238536162694	37.0	37.0	37.0	35.0	37.0
5	36.357243582119985	37.0	37.0	37.0	35.0	37.0
6	36.43386737797108	37.0	37.0	37.0	35.0	37.0
7	36.35611376340067	37.0	37.0	37.0	35.0	37.0
8	36.393661122343225	37.0	37.0	37.0	35.0	37.0
9	38.16345843456396	39.0	39.0	39.0	37.0	39.0
10	38.1262933451129	39.0	39.0	39.0	37.0	39.0
11	38.16435039671078	39.0	39.0	39.0	37.0	39.0
12	38.083835946924005	39.0	39.0	39.0	37.0	39.0
13	38.12646324266467	39.0	39.0	39.0	37.0	39.0
14	39.53399650011043	41.0	39.0	41.0	37.0	41.0
15	39.501197777740025	41.0	39.0	41.0	37.0	41.0
16	39.42290049100392	41.0	39.0	41.0	36.0	41.0
17	39.36939125707199	41.0	39.0	41.0	36.0	41.0
18	39.3047537334987	41.0	39.0	41.0	36.0	41.0
19	39.32323009225437	41.0	39.0	41.0	36.0	41.0
20	39.506897840602115	41.0	39.0	41.0	37.0	41.0
21	39.51527378990469	41.0	39.0	41.0	37.0	41.0
22	39.52298713875533	41.0	39.0	41.0	37.0	41.0
23	39.44982075808288	41.0	39.0	41.0	37.0	41.0
24	39.41234985303862	41.0	39.0	41.0	36.0	41.0
25	39.408348765694285	41.0	39.0	41.0	36.0	41.0
26	39.236769228155424	41.0	39.0	41.0	36.0	41.0
27	39.131984913097405	41.0	39.0	41.0	35.0	41.0
28	39.09185511136784	41.0	39.0	41.0	35.0	41.0
29	38.9876229633531	40.0	39.0	41.0	35.0	41.0
30	38.87488744287195	40.0	39.0	41.0	35.0	41.0
31	38.71819942574628	40.0	38.0	41.0	35.0	41.0
32	38.61638831784434	40.0	38.0	41.0	35.0	41.0
33	38.52677585415994	40.0	38.0	41.0	35.0	41.0
34	38.47312220730899	40.0	38.0	41.0	35.0	41.0
35	38.30679250412002	40.0	38.0	41.0	35.0	41.0
36	38.30382779184152	40.0	38.0	41.0	35.0	41.0
37	38.22201362578365	40.0	38.0	41.0	34.0	41.0
38	38.12490018518833	40.0	38.0	41.0	34.0	41.0
39	38.067236956115465	40.0	38.0	41.0	34.0	41.0
40	37.952479654768176	40.0	38.0	41.0	34.0	41.0
41	37.806529162914764	40.0	38.0	41.0	33.0	41.0
42	37.694354304354476	40.0	37.0	41.0	33.0	41.0
43	37.38232046076216	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	5.0
20	6.0
21	24.0
22	41.0
23	80.0
24	128.0
25	181.0
26	266.0
27	430.0
28	553.0
29	719.0
30	932.0
31	1236.0
32	1583.0
33	2117.0
34	2969.0
35	4195.0
36	6399.0
37	11655.0
38	23852.0
39	60344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.600808712346456	19.40739733940434	12.71258431166007	24.279209636589137
2	19.925584872321988	21.361219184831544	34.70412341358161	24.009072529264852
3	18.509488778266707	25.358059090368506	30.20183829151022	25.930613839854566
4	13.354797057374403	17.07555344127491	37.895648923699014	31.67400057765168
5	12.68794916665251	38.17682937188875	35.9554188824139	13.179802579044836
6	30.975721639851166	38.193819127066384	15.57535805909037	15.255101173992083
7	27.787594080769296	30.10584617475662	24.123753376713843	17.98280636776024
8	25.686810853055608	34.547817665947434	19.59003720756384	20.175334273433123
9	25.015715523539306	14.730967226762262	20.679080514449787	39.57423673524865
10	17.897857591872103	26.146383730610438	34.025382694235375	21.93037598328208
11	33.31011400125724	24.437214359741077	22.26422467252247	19.988446966479213
12	20.087837034268336	28.592058988429976	28.717783176744422	22.602320800557262
13	29.29968229157818	20.892301941929016	25.45235223160434	24.35566353488846
14	22.132554069895853	22.006829881581407	26.183761192001224	29.676854856521516
15	24.016717919094784	29.472977794389983	23.340525663025197	23.169778623490036
16	22.568341290202007	26.204998385973262	26.65692587369816	24.569734450126575
17	21.72904738442719	28.654921082587197	26.581321463157714	23.034710069827895
18	21.906590326033403	26.905825787050407	27.2303301109431	23.95725377597309
19	23.16638067245451	26.051241101615723	29.198593248271294	21.58378497765847
20	22.878404322193717	26.410574423622556	28.620941572231946	22.09007968195178
21	22.067993000220866	27.170865967821406	28.110399429144227	22.650741602813504
22	22.558147437095432	26.082672148694336	28.500314310470788	22.858866103739444
23	22.351721911687253	27.13263901867174	28.29813622385701	22.217502845783994
24	21.050306665080956	27.979578314276495	27.42316383220918	23.546951188433376
25	22.646494164019096	26.727433357685314	28.902122020421682	21.723950457873904
26	21.776618698924548	27.441852562904568	28.323620856623457	22.457907881547428
27	21.35272430724273	27.73577532747753	28.353352928184307	22.558147437095432
28	21.587182928694	26.692604359571177	29.913012453490545	21.80720025824428
29	22.364464228070474	27.491972340678572	28.61329618240201	21.530267248848943
30	21.66873375354661	28.06282811464687	27.683956574185764	22.584481557620755
31	22.665182894714487	27.12584311660069	28.355901391460947	21.853072597223875
32	21.766424845817973	27.287245790788155	28.33041675869451	22.615912604699368
33	21.049457177322076	28.231026690905388	27.84705822389099	22.872457907881547
34	22.748432695084865	25.68341290202008	28.705890348120082	22.86226405477497
35	21.979646273297202	26.650129971627106	28.864744559030903	22.505479196044785
36	22.42392837119217	26.62889277765507	27.821573591124555	23.125605260028202
37	21.57613958782854	25.544096909563535	29.54858220493043	23.3311812976775
38	21.57104266127525	25.9883790074585	30.068468713365842	22.372109617900406
39	20.84048318863725	25.99262644625291	30.293582969469412	22.87330739564043
40	20.98064866885268	25.540698958528008	30.845750012742318	22.632902359876994
41	20.944120695220782	24.81778487571994	30.278292189809548	23.959802239249733
42	19.46176455597275	26.195654020625565	29.956336329193494	24.386245094208192
43	20.123515520141353	24.852613873834077	31.385174739632006	23.638695866392563
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	143.5
2	128.0
3	183.0
4	238.0
5	238.0
6	238.5
7	239.0
8	210.0
9	181.0
10	266.5
11	352.0
12	352.0
13	586.5
14	821.0
15	1082.0
16	1343.0
17	1245.5
18	1148.0
19	1148.0
20	1220.5
21	1293.0
22	963.0
23	633.0
24	664.0
25	695.0
26	695.0
27	885.5
28	1076.0
29	1573.5
30	2071.0
31	2779.5
32	3488.0
33	3488.0
34	4217.0
35	4946.0
36	5583.0
37	6220.0
38	6861.0
39	7502.0
40	7502.0
41	8680.0
42	9858.0
43	10400.0
44	10942.0
45	11258.0
46	11574.0
47	11574.0
48	11441.5
49	11309.0
50	10938.5
51	10568.0
52	9323.0
53	8078.0
54	8078.0
55	7507.5
56	6937.0
57	5652.5
58	4368.0
59	3828.5
60	3289.0
61	3289.0
62	2967.5
63	2646.0
64	2298.0
65	1950.0
66	1640.5
67	1331.0
68	1331.0
69	1130.0
70	929.0
71	767.0
72	605.0
73	490.0
74	375.0
75	375.0
76	282.0
77	189.0
78	152.0
79	115.0
80	92.5
81	70.0
82	70.0
83	48.0
84	26.0
85	20.0
86	14.0
87	9.5
88	5.0
89	5.0
90	4.0
91	3.0
92	3.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	117718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.53463361592959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00609094409634	37.85997043782599
2	9.568733153638814	9.288299155609167
3	3.5670528931984453	5.193768157800846
4	1.9585535758042498	3.802307208753122
5	1.3144537403297512	3.1898265345996366
6	0.9608989393355969	2.7982126777553136
7	0.7491161129975146	2.545065325608658
8	0.5338327440753317	2.0727501316706025
9	0.4078132110477124	1.7813758303742842
>10	2.7794308117758253	24.75067534276831
>50	0.13652116077992088	4.317946278394128
>100	0.014002170336402142	1.2691347117688034
>500	0.0035005425841005355	1.130668207071136
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.5793506515571111	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	649	0.5513175555140251	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	469	0.39840975891537406	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	312	0.26504018077099506	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	140	0.11892828624339524	No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATT	121	0.10278801882464873	No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAG	119	0.10108904330688594	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.10023955554800455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.494877588813945E-4	0.0
10	0.0	0.0	0.0	8.494877588813945E-4	0.0
11	0.0	0.0	0.0	8.494877588813945E-4	0.0
12	0.0	0.0	0.0	8.494877588813945E-4	0.0
13	0.0	0.0	0.0	8.494877588813945E-4	0.0
14	0.0	0.0	0.0	8.494877588813945E-4	0.0
15	0.0	0.0	0.0	8.494877588813945E-4	0.0
16	0.0	0.0	0.0	8.494877588813945E-4	0.0
17	0.0	0.0	0.0	8.494877588813945E-4	0.0
18	0.0	0.0	0.0	8.494877588813945E-4	0.0
19	0.0	0.0	0.0	8.494877588813945E-4	0.0
20	0.0	0.0	0.0	8.494877588813945E-4	0.0
21	0.0	0.0	0.0	0.001698975517762789	0.0
22	0.0	0.0	0.0	0.0025484632766441836	0.0
23	0.0	0.0	0.0	0.003397951035525578	0.0
24	0.0	0.0	0.0	0.005946414312169762	0.0
25	0.0	0.0	0.0	0.01868873069539068	0.0
26	0.0	0.0	0.0	0.0314310470786116	0.0
27	0.0	0.0	0.0	0.10533648210129291	0.0
28	0.0	0.0	0.0	0.23700708472790907	0.0
29	0.0	0.0	0.0	0.3720756383900508	0.0
30	0.0	0.0	0.0	0.5411237024074483	0.0
31	0.0	0.0	0.0	0.8588321242290898	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGT	20	0.0018352327	37.0	34
CATCGTT	20	0.0018352327	37.0	32
CTAATAA	25	1.2269052E-4	37.0	24
ACGACCA	30	3.580055E-4	30.833332	11
GTCGATG	25	0.0054765693	29.6	21
GGTCGAT	25	0.0054765693	29.6	20
CATGCGG	25	0.0054765693	29.6	26
AACCACG	25	0.0054765693	29.6	7
CGACCAA	25	0.0054765693	29.6	12
ACCACGA	25	0.0054765693	29.6	8
CTCTAGC	25	0.0054765693	29.6	2
GGTATCA	210	0.0	29.071428	1
TCGATGA	35	8.8219834E-4	26.428572	22
AAAACCA	35	8.8219834E-4	26.428572	27
GAAAAAC	35	8.8219834E-4	26.428572	25
TGGATGG	35	8.8219834E-4	26.428572	5
TATTAAC	40	0.0019209622	23.125	2
TTAACCC	40	0.0019209622	23.125	16
ATAAAAC	40	0.0019209622	23.125	27
TGAGCCG	40	0.0019209622	23.125	10
>>END_MODULE