FastQCFastQC Report
Fri 10 Feb 2017
ERR1630050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143810
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7950.5528127390306655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.4346012099297685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4890.3400319866490508No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG4750.33029691954662405No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG3960.2753633266115013No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT3910.2718865169320631No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC3670.25519783047076No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG3490.2426813156247827No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA3340.23225088658646825No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.22321118141992907No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC2780.193310618176761No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA2620.18218482720255894No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG2590.18009874139489604No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC2580.17940337945900842No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC2550.17731729365134552No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT2390.16619150267714347No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT2230.15506571170294137No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA2160.15019817815172798No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG2130.14811209234406508No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC2120.14741673040817746No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA2060.14324455879285167No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG2020.14046311104930115No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG2010.13976774911341353No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA2000.1390723871775259No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA2000.1390723871775259No Hit
CTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGA1990.13837702524163828No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG1940.1349002155622001No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT1930.13420485362631251No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC1870.13003268201098672No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG1830.1272512342674362No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG1810.12586051039566096No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA1800.1251651484597733No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC1780.12377442458799805No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA1780.12377442458799805No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTAC1760.1223837007162228No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC1760.1223837007162228No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC1740.12099297684444754No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA1690.11751616716500939No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC1690.11751616716500939No Hit
GAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCC1670.11612544329323413No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG1660.11543008135734649No Hit
TTCTTAACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCC1620.11264863361379598No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC1610.11195327167790835No Hit
CTTTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATC1580.10986718587024547No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA1580.10986718587024547No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC1550.10778110006258257No Hit
CCCCATCTCTCTTCCCTAATCCTCCAAGTCTTCAGCGAGACCC1500.10430429038314443No Hit
TTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATCAG1460.10152284263959391No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.10082748070370628No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT1440.10013211876781865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2050.029.7804871
AGATAGT250.005480563729.68
GTAGGGG250.005480563729.63
TATAGAT358.831558E-426.428575
GACTGGG450.00380979520.5555577
GTATCAA3050.020.0163942
GTATTAT609.1833563E-418.51
AGGGGCT701.2098116E-418.55
AAAGGAT500.007005720418.531
AACTGGC852.6963906E-517.4117647
ACTGGCC904.4026005E-516.4444458
GCCAAGT956.991269E-515.57894812
GAGTACG2950.015.0508471
CAAGTAC1001.0828371E-414.814
TACGGGG1507.932249E-814.7999994
GGACGAT1405.8894875E-714.53571412
TGCCCTG2101.3096724E-1014.09523828
GAACTGG1051.639793E-414.0952386
CTGGCCA1051.639793E-414.0952389
AGTACGG3150.014.0952382
Produced by FastQC (version 0.11.5)