##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630050.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	143810
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.761469995132465	34.0	31.0	34.0	31.0	34.0
2	32.98955566372297	34.0	31.0	34.0	31.0	34.0
3	33.11934496905639	34.0	33.0	34.0	31.0	34.0
4	36.43795980808011	37.0	37.0	37.0	35.0	37.0
5	36.388630832348234	37.0	37.0	37.0	35.0	37.0
6	36.4265975940477	37.0	37.0	37.0	35.0	37.0
7	36.36431402545025	37.0	37.0	37.0	35.0	37.0
8	36.370391488769904	37.0	37.0	37.0	35.0	37.0
9	38.14303595021209	39.0	39.0	39.0	37.0	39.0
10	38.125359849801825	39.0	39.0	39.0	37.0	39.0
11	38.16597594047702	39.0	39.0	39.0	37.0	39.0
12	38.08500799666226	39.0	38.0	39.0	37.0	39.0
13	38.10232946248522	39.0	39.0	39.0	37.0	39.0
14	39.51732841944232	41.0	39.0	41.0	37.0	41.0
15	39.49094638759474	41.0	39.0	41.0	37.0	41.0
16	39.41514498296363	41.0	39.0	41.0	36.0	41.0
17	39.33702106946666	41.0	39.0	41.0	36.0	41.0
18	39.229844934288295	40.0	39.0	41.0	36.0	41.0
19	39.26891732146582	41.0	39.0	41.0	36.0	41.0
20	39.50171754398164	41.0	39.0	41.0	37.0	41.0
21	39.51045128989639	41.0	39.0	41.0	37.0	41.0
22	39.5373687504346	41.0	39.0	41.0	37.0	41.0
23	39.48682289131493	41.0	39.0	41.0	37.0	41.0
24	39.42696613587372	41.0	39.0	41.0	36.0	41.0
25	39.40952645852166	41.0	39.0	41.0	36.0	41.0
26	39.27285307002295	41.0	39.0	41.0	36.0	41.0
27	39.16005841040261	41.0	39.0	41.0	36.0	41.0
28	39.1372505389055	41.0	39.0	41.0	35.0	41.0
29	39.012161880258674	40.0	39.0	41.0	35.0	41.0
30	38.93777901397677	40.0	39.0	41.0	35.0	41.0
31	38.753584590779504	40.0	38.0	41.0	35.0	41.0
32	38.640393574855715	40.0	38.0	41.0	35.0	41.0
33	38.58236562130589	40.0	38.0	41.0	35.0	41.0
34	38.43631875391141	40.0	38.0	41.0	35.0	41.0
35	38.33495584451707	40.0	38.0	41.0	35.0	41.0
36	38.297420207217854	40.0	38.0	41.0	34.0	41.0
37	38.20104999652319	40.0	38.0	41.0	34.0	41.0
38	38.13218830401224	40.0	38.0	41.0	34.0	41.0
39	37.991349697517556	40.0	38.0	41.0	34.0	41.0
40	37.93167373617968	40.0	38.0	41.0	34.0	41.0
41	37.80365760378277	40.0	37.0	41.0	33.0	41.0
42	37.71634100549336	40.0	37.0	41.0	33.0	41.0
43	37.39429803212572	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	5.0
20	7.0
21	27.0
22	45.0
23	95.0
24	164.0
25	231.0
26	355.0
27	472.0
28	700.0
29	956.0
30	1210.0
31	1531.0
32	1948.0
33	2629.0
34	3604.0
35	4893.0
36	7604.0
37	14286.0
38	28016.0
39	75027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83832834990613	19.505597663583895	12.064529587650371	23.591544398859607
2	19.24066476601071	21.699464571309367	35.06710242681316	23.99276823586677
3	18.99102983102705	22.844725679716294	30.88380502051318	27.280439468743484
4	12.790487448717059	16.460607746331966	37.37014115847298	33.37876364647799
5	14.243793894722204	36.41819066824282	35.097698351992214	14.240317085042765
6	32.85932828036993	37.184479521590994	15.35359154439886	14.602600653640218
7	28.971559696822197	30.429038314442668	21.82741116751269	18.771990821222445
8	25.909185731173075	34.123496279813644	20.374800083443432	19.59251790556985
9	24.69299770530561	13.989986788123218	21.157082261317015	40.15993324525415
10	16.5822960851123	26.809679438147555	34.98504971837841	21.622974758361728
11	33.56581600723176	23.242472707044016	21.76621931715458	21.42549196856964
12	19.842848202489396	26.425839649537586	29.956192198039076	23.77511994993394
13	29.81572908698978	20.24893957304777	25.996801335094915	23.938530004867534
14	21.66886864613031	22.63403101314234	26.275641471385857	29.421458869341492
15	23.11800292052013	29.032751547180307	24.859189207982755	22.990056324316807
16	24.29177386829845	26.669911689034144	25.598358945831308	23.439955496836102
17	22.098602322508864	27.03428134343926	26.850705792364927	24.016410541686948
18	21.56317363187539	25.638689938112787	28.699673179890134	24.09846325012169
19	25.030943606146998	26.00166886864613	28.253250817050272	20.714136708156595
20	24.3675683193102	26.27425074751408	27.729643279326886	21.628537653848827
21	21.73284194423197	26.995341075029554	27.667060705097	23.604756275641474
22	23.619358876295113	25.593491412280088	28.007788053681942	22.779361657742854
23	21.347611431750227	26.10388707322161	28.63222307210903	23.91627842291913
24	21.67860371323274	27.147625338988945	27.877060009735068	23.29671093804325
25	22.910089701689728	26.534316111536054	28.164939851192543	22.39065433558167
26	23.387803351644532	27.160837215770812	26.250608441693903	23.200750990890757
27	22.512342674362003	26.9814338363118	26.50302482442111	24.003198664905085
28	22.51721020791322	26.710938043251513	27.990404005284752	22.78144774355052
29	22.524859189207984	27.584312634726377	28.610666852096518	21.280161323969125
30	23.266115012864198	26.22070787845073	26.845838258813714	23.66733884987136
31	21.764133231346918	25.95229817119811	28.42778666295807	23.855781934496907
32	21.55761073638829	26.83749391558306	28.011960225297265	23.592935122731383
33	22.37605173492803	26.04617203254294	27.90765593491412	23.670120297614908
34	23.68680898407621	25.73464988526528	28.22265489187122	22.35588623878729
35	23.257770669633544	25.92517905569849	27.267227591961618	23.54982268270635
36	22.204297336763787	25.525345942563106	26.799944371045132	25.47041234962798
37	21.476948751825326	24.187469577915305	30.75585842430985	23.579723245949516
38	21.44843891245393	23.9684305681107	29.51880954036576	25.064320979069603
39	20.560461720325428	25.930741951185592	30.801752312078435	22.707044016410542
40	22.105555941867742	23.9684305681107	30.189833808497323	23.736179681524234
41	20.509004937069744	24.834851540226687	30.397747027327725	24.25839649537584
42	19.672484528196925	26.39732981016619	29.650232946248522	24.279952715388358
43	20.61052777970934	24.35366108059245	29.801126486336138	25.234684653362077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	191.0
1	149.0
2	107.0
3	172.0
4	237.0
5	237.0
6	303.5
7	370.0
8	335.5
9	301.0
10	385.0
11	469.0
12	469.0
13	712.0
14	955.0
15	1221.0
16	1487.0
17	1504.5
18	1522.0
19	1522.0
20	2000.5
21	2479.0
22	2385.5
23	2292.0
24	2623.0
25	2954.0
26	2954.0
27	2937.0
28	2920.0
29	3170.5
30	3421.0
31	3909.0
32	4397.0
33	4397.0
34	5668.5
35	6940.0
36	7633.0
37	8326.0
38	7859.0
39	7392.0
40	7392.0
41	7769.0
42	8146.0
43	8764.0
44	9382.0
45	8908.0
46	8434.0
47	8434.0
48	8191.5
49	7949.0
50	8402.0
51	8855.0
52	9495.5
53	10136.0
54	10136.0
55	10252.0
56	10368.0
57	8766.0
58	7164.0
59	7033.0
60	6902.0
61	6902.0
62	6323.0
63	5744.0
64	4516.5
65	3289.0
66	3039.5
67	2790.0
68	2790.0
69	2567.0
70	2344.0
71	2604.5
72	2865.0
73	2539.5
74	2214.0
75	2214.0
76	1238.5
77	263.0
78	181.5
79	100.0
80	77.5
81	55.0
82	55.0
83	40.5
84	26.0
85	17.5
86	9.0
87	9.5
88	10.0
89	10.0
90	7.0
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	143810.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.05069188512621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.0945298027678	43.44134622070788
2	7.033889070502343	7.181698073847438
3	2.228397079655661	3.4128363813364855
4	1.2381497221314155	2.528335998887421
5	0.8172605426610003	2.0860858076628888
6	0.5639097744360901	1.7272790487448717
7	0.42088917947041515	1.5040678673249426
8	0.34188732701318514	1.3962867672623602
9	0.24517816279830007	1.1264863361379598
>10	1.6835567178816606	15.957861066685208
>50	0.20431513566525009	7.399346359780265
>100	0.12531328320802004	11.250956122661846
>500	0.002724201808870001	0.9874139489604339
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	795	0.5528127390306655	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	625	0.4346012099297685	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	489	0.3400319866490508	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	475	0.33029691954662405	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	396	0.2753633266115013	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	391	0.2718865169320631	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	367	0.25519783047076	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	349	0.2426813156247827	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	334	0.23225088658646825	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	321	0.22321118141992907	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	278	0.193310618176761	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	262	0.18218482720255894	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	259	0.18009874139489604	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	258	0.17940337945900842	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	255	0.17731729365134552	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	239	0.16619150267714347	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	223	0.15506571170294137	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	216	0.15019817815172798	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	213	0.14811209234406508	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	212	0.14741673040817746	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	206	0.14324455879285167	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	202	0.14046311104930115	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	201	0.13976774911341353	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	200	0.1390723871775259	No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA	200	0.1390723871775259	No Hit
CTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGA	199	0.13837702524163828	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	194	0.1349002155622001	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	193	0.13420485362631251	No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC	187	0.13003268201098672	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	183	0.1272512342674362	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	181	0.12586051039566096	No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA	180	0.1251651484597733	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	178	0.12377442458799805	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	178	0.12377442458799805	No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTAC	176	0.1223837007162228	No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC	176	0.1223837007162228	No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC	174	0.12099297684444754	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	169	0.11751616716500939	No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC	169	0.11751616716500939	No Hit
GAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCC	167	0.11612544329323413	No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG	166	0.11543008135734649	No Hit
TTCTTAACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCC	162	0.11264863361379598	No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC	161	0.11195327167790835	No Hit
CTTTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATC	158	0.10986718587024547	No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA	158	0.10986718587024547	No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC	155	0.10778110006258257	No Hit
CCCCATCTCTCTTCCCTAATCCTCCAAGTCTTCAGCGAGACCC	150	0.10430429038314443	No Hit
TTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATCAG	146	0.10152284263959391	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	145	0.10082748070370628	No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT	144	0.10013211876781865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.953619358876295E-4	0.0
9	0.0	0.0	0.0	6.953619358876295E-4	0.0
10	0.0	0.0	0.0	6.953619358876295E-4	0.0
11	0.0	0.0	0.0	6.953619358876295E-4	0.0
12	0.0	0.0	0.0	6.953619358876295E-4	0.0
13	0.0	0.0	0.0	6.953619358876295E-4	0.0
14	0.0	0.0	0.0	6.953619358876295E-4	0.0
15	0.0	0.0	0.0	6.953619358876295E-4	0.0
16	0.0	0.0	0.0	6.953619358876295E-4	0.0
17	0.0	0.0	0.0	0.001390723871775259	0.0
18	0.0	0.0	0.0	0.001390723871775259	0.0
19	0.0	0.0	0.0	0.001390723871775259	0.0
20	0.0	0.0	0.0	0.001390723871775259	0.0
21	0.0	0.0	0.0	0.001390723871775259	0.0
22	0.0	0.0	0.0	0.0020860858076628885	0.0
23	0.0	0.0	0.0	0.0062582574229886655	0.0
24	0.0	0.0	0.0	0.009039705166539184	0.0
25	0.0	0.0	0.0	0.020165496140741257	0.0
26	0.0	0.0	0.0	0.038244906473819625	0.0
27	0.0	0.0	0.0	0.11612544329323413	0.0
28	0.0	0.0	0.0	0.273972602739726	0.0
29	0.0	0.0	0.0	0.44850844864752104	0.0
30	0.0	0.0	0.0	0.6223489326194284	0.0
31	0.0	0.0	0.0	1.0326124747931298	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	205	0.0	29.780487	1
AGATAGT	25	0.0054805637	29.6	8
GTAGGGG	25	0.0054805637	29.6	3
TATAGAT	35	8.831558E-4	26.42857	5
GACTGGG	45	0.003809795	20.555557	7
GTATCAA	305	0.0	20.016394	2
GTATTAT	60	9.1833563E-4	18.5	1
AGGGGCT	70	1.2098116E-4	18.5	5
AAAGGAT	50	0.0070057204	18.5	31
AACTGGC	85	2.6963906E-5	17.411764	7
ACTGGCC	90	4.4026005E-5	16.444445	8
GCCAAGT	95	6.991269E-5	15.578948	12
GAGTACG	295	0.0	15.050847	1
CAAGTAC	100	1.0828371E-4	14.8	14
TACGGGG	150	7.932249E-8	14.799999	4
GGACGAT	140	5.8894875E-7	14.535714	12
TGCCCTG	210	1.3096724E-10	14.095238	28
GAACTGG	105	1.639793E-4	14.095238	6
CTGGCCA	105	1.639793E-4	14.095238	9
AGTACGG	315	0.0	14.095238	2
>>END_MODULE