FastQCFastQC Report
Fri 10 Feb 2017
ERR1630049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14841
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5673.820497271073378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4152.796307526447005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3052.055117579677919No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1801.2128562765312312No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.39080924466006334No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.3840711542348898No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.3638568829593693No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.35038070210902234No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.28299979785728724No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA400.2695236170069402No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.26278552658176674No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA360.2425712553062462No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG340.22909507445589922No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.2156188936055522No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA280.18866653190485816No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTGTCT270.18192844147968465No Hit
CAGTTGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA250.16845226062933766No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA240.16171417020416412No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.16171417020416412No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC230.15497607977899064No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACCTGTC230.15497607977899064No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.14823798935381713No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT210.14149989892864362No Hit
CAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.14149989892864362No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.14149989892864362No Hit
GAGTACGGGGGAGGCAGTACAGGCACAAAAAAAAAAAAAAAAA200.1347618085034701No Hit
GAGTACGGGGGAGGCAGTACAGGCAAAAAAAAAAAAAAAAAAA200.1347618085034701No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT200.1347618085034701RNA PCR Primer, Index 46 (95% over 21bp)
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA190.1280237180782966No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT190.1280237180782966No Hit
GCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1212856276531231No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA180.1212856276531231No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11454753722794961No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA160.1078094468027761No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1078094468027761No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTAAAA160.1078094468027761No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA160.1078094468027761No Hit
GTACAGGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1010713563776026No Hit
TATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTAAAAAA150.1010713563776026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3000.013.5666671
AGTACGG2501.0186341E-1012.582
GAGTACG2501.0186341E-1012.581
CGCAGAG3400.011.4264719
AGTACTT1951.5674959E-611.38461614
GAGTACT1951.5674959E-611.38461613
AGAGTAC3451.8189894E-1211.26086912
CAGAGTA3501.8189894E-1211.111
GCAGAGT3551.8189894E-1210.94366310
GTACGGG2901.2223609E-910.8448283
AACGCAG3601.8189894E-1210.7916665
GTATCAA3601.8189894E-1210.7916662
ACGCAGA3901.2732926E-119.9615388
GTACTTT2301.133492E-59.65217415
TACTTTT2351.4636626E-59.44680916
ACTTTTT2452.3984156E-59.06122517
TATCAAC4552.928573E-108.5384623
CTTTTTT2656.044805E-58.37735818
TCAACGC4901.3460522E-108.3061235
ATCAACG4808.440111E-108.093754
Produced by FastQC (version 0.11.5)