FastQCFastQC Report
Fri 10 Feb 2017
ERR1630048.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630048.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139534
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22140.19428994659220347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.17945932284600546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17070.14979807535360945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.1107470246609579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.026.4285721
GCGGTTA450.003825787220.55555732
TCGTATT500.00703484618.524
TAGCACA1653.6379788E-1217.9393944
GTATCAA8900.017.2528082
CTAGCCT1352.2189852E-816.4444454
TGCGCAA700.002592474515.85714310
CGCACGA700.002592474515.85714328
TATAGAC855.3640996E-415.2352943
GTTCTAC1405.9941885E-714.5357151
TAAGCTA1304.443589E-614.23076933
AGCGATA800.006298577413.87522
GGTTAGA800.006298577413.8751
TGTACTG2850.013.6315785
GTCTAAT1102.4563298E-413.4545451
TCAACGC11900.013.3697485
TAGGGTC1254.8443093E-513.325
TACACTT3350.013.2537325
TAATACT2950.013.1694924
GTATATA2254.0563464E-1013.1555561
Produced by FastQC (version 0.11.5)