##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630048.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1139534
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.859752319807924	34.0	31.0	34.0	31.0	34.0
2	33.0918340304019	34.0	33.0	34.0	31.0	34.0
3	33.22370723471173	34.0	33.0	34.0	31.0	34.0
4	36.499949979553044	37.0	37.0	37.0	35.0	37.0
5	36.44925645044378	37.0	37.0	37.0	35.0	37.0
6	36.49032850270374	37.0	37.0	37.0	35.0	37.0
7	36.42256483790742	37.0	37.0	37.0	35.0	37.0
8	36.422148878401174	37.0	37.0	37.0	35.0	37.0
9	38.20899244779006	39.0	39.0	39.0	37.0	39.0
10	38.17348319576248	39.0	39.0	39.0	37.0	39.0
11	38.21149171503439	39.0	39.0	39.0	37.0	39.0
12	38.14083915003852	39.0	39.0	39.0	37.0	39.0
13	38.1581198981338	39.0	39.0	39.0	37.0	39.0
14	39.61844578573346	41.0	40.0	41.0	37.0	41.0
15	39.60398636635677	41.0	40.0	41.0	37.0	41.0
16	39.52798424619186	41.0	39.0	41.0	37.0	41.0
17	39.44513546765608	41.0	39.0	41.0	37.0	41.0
18	39.335435362174366	41.0	39.0	41.0	36.0	41.0
19	39.35356207010936	41.0	39.0	41.0	36.0	41.0
20	39.5813911651605	41.0	39.0	41.0	37.0	41.0
21	39.60116767029329	41.0	40.0	41.0	37.0	41.0
22	39.61534364047058	41.0	40.0	41.0	37.0	41.0
23	39.55004063064376	41.0	40.0	41.0	37.0	41.0
24	39.52102613875496	41.0	40.0	41.0	37.0	41.0
25	39.49776487581766	41.0	40.0	41.0	37.0	41.0
26	39.35639041924155	41.0	39.0	41.0	36.0	41.0
27	39.278106664654146	41.0	39.0	41.0	36.0	41.0
28	39.23646771399537	41.0	39.0	41.0	36.0	41.0
29	39.13272355190806	41.0	39.0	41.0	36.0	41.0
30	39.05720935048888	41.0	39.0	41.0	36.0	41.0
31	38.934770704516055	40.0	39.0	41.0	35.0	41.0
32	38.85510217334454	40.0	39.0	41.0	35.0	41.0
33	38.78147734073753	40.0	39.0	41.0	35.0	41.0
34	38.71508177904301	40.0	39.0	41.0	35.0	41.0
35	38.59193933660602	40.0	38.0	41.0	35.0	41.0
36	38.593391684671104	40.0	38.0	41.0	35.0	41.0
37	38.54887611953658	40.0	38.0	41.0	35.0	41.0
38	38.460540887766406	40.0	38.0	41.0	35.0	41.0
39	38.41827536519314	40.0	38.0	41.0	35.0	41.0
40	38.36218752577808	40.0	38.0	41.0	34.0	41.0
41	38.26345944921345	40.0	38.0	41.0	34.0	41.0
42	38.176417728650485	40.0	38.0	41.0	34.0	41.0
43	37.88396835899587	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	0.0
15	2.0
16	2.0
17	15.0
18	21.0
19	57.0
20	107.0
21	231.0
22	421.0
23	683.0
24	1203.0
25	1616.0
26	2563.0
27	3438.0
28	4788.0
29	6371.0
30	8349.0
31	10794.0
32	13912.0
33	18149.0
34	25055.0
35	35233.0
36	53101.0
37	93952.0
38	210050.0
39	649418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56930113537639	18.835857464542524	11.632913103075468	25.961928297005617
2	18.5869838021507	20.629924161982004	35.40605194755049	25.377040088316804
3	18.826730926852555	23.203081259532404	30.270882659051857	27.69930515456318
4	13.9012087397129	15.475185470551997	36.01937283135036	34.60423295838474
5	14.181586508169127	36.52852832824646	35.2029864839487	14.086898679635713
6	34.4509246762273	36.080099409056686	15.434291561287333	14.034684353428684
7	29.007998006202534	30.35740925676636	22.276913194340846	18.35767954269026
8	26.634396165450084	34.66873300840519	19.93595627686405	18.76091454928067
9	26.137965168217885	14.404484640212578	19.827052110775107	39.63049808079443
10	16.071657361693465	26.623690034698395	34.726651420668446	22.578001182939694
11	35.302149826157006	21.987847664045127	22.25944991549177	20.450552594306092
12	20.687403798394783	25.1722195213131	31.26672832929952	22.8736483509926
13	30.02806410339665	20.457660763083858	24.897194818232716	24.617080315286774
14	21.86604348795209	20.633346613615743	26.159728450401655	31.340881448030512
15	24.359255625545178	28.39862610505698	22.768693167557966	24.473425101839872
16	24.14504525534122	26.89757392056753	24.495978180554506	24.461402643536744
17	23.14744448169164	26.84492081851002	26.2687203716607	23.738914328137643
18	23.381926296187743	26.308999994734688	26.74347584187922	23.565597867198345
19	24.161982003169715	25.76877916762466	26.92942904731232	23.1398097818933
20	23.97708185977777	25.908924174267728	26.82280651564587	23.291187450308634
21	23.718818394185693	25.925509901415843	26.553222633111428	23.802449071287036
22	23.90924711329368	25.702611769372393	26.71785133221124	23.67028978512269
23	23.77893068570135	25.878736395754753	26.561822639780825	23.780510278763074
24	23.74444290385368	26.076624304320887	26.46239603206223	23.7165367597632
25	24.147414644933807	25.523327956866577	26.65238597531974	23.676871422879877
26	23.95830225337726	25.943850731965874	26.524965468340568	23.572881546316303
27	23.654493854505436	26.093824317659674	26.459500111449064	23.792181716385823
28	23.712675532278986	25.895058857392584	26.534179761200633	23.858085849127804
29	23.633257103342242	26.05775694275028	26.77673505134555	23.532250902561923
30	23.592099928567293	26.0974222796336	26.4418613222598	23.8686164695393
31	23.9320634575186	25.78466285341201	26.61149206605507	23.671781623014322
32	23.44572430484742	25.957979314351302	26.792092206112322	23.80420417468895
33	23.318216042698158	25.886985381743767	27.04096586850414	23.753832707053938
34	23.72733064568499	25.23005017840626	26.931184150714238	24.11143502519451
35	23.396932430274127	25.633109674656485	27.140831252073216	23.829126642996172
36	23.560771332843075	25.481380985560765	26.910386175401523	24.047461506194637
37	23.302069091400522	24.986792846900574	27.39909471766529	24.31204334403361
38	23.05249338764793	25.21486853397968	27.718435781644075	24.014202296728314
39	22.901642250253175	25.017331646093933	28.03198500439653	24.049041099256364
40	22.790017673891256	24.79469677956077	28.31701379686784	24.098271749680134
41	22.513501132919245	24.654464017747603	28.49489352665212	24.337141322681024
42	22.110353881498927	25.01434797031067	28.37633629185263	24.49896185633777
43	22.19670496887324	24.62629460814684	28.67057937718401	24.50642104579591
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	397.0
1	387.5
2	378.0
3	747.5
4	1117.0
5	1117.0
6	1268.0
7	1419.0
8	1197.5
9	976.0
10	1338.5
11	1701.0
12	1701.0
13	2695.5
14	3690.0
15	4728.5
16	5767.0
17	6372.0
18	6977.0
19	6977.0
20	8587.0
21	10197.0
22	10911.0
23	11625.0
24	14037.5
25	16450.0
26	16450.0
27	19475.0
28	22500.0
29	26243.5
30	29987.0
31	33923.5
32	37860.0
33	37860.0
34	42734.5
35	47609.0
36	51448.5
37	55288.0
38	59391.5
39	63495.0
40	63495.0
41	67772.5
42	72050.0
43	74043.0
44	76036.0
45	77270.5
46	78505.0
47	78505.0
48	78993.0
49	79481.0
50	79799.5
51	80118.0
52	78292.0
53	76466.0
54	76466.0
55	74436.5
56	72407.0
57	68647.5
58	64888.0
59	61371.0
60	57854.0
61	57854.0
62	52878.0
63	47902.0
64	42657.0
65	37412.0
66	32298.5
67	27185.0
68	27185.0
69	23431.5
70	19678.0
71	16318.0
72	12958.0
73	10483.0
74	8008.0
75	8008.0
76	6537.0
77	5066.0
78	3994.0
79	2922.0
80	2294.0
81	1666.0
82	1666.0
83	1216.5
84	767.0
85	596.5
86	426.0
87	294.0
88	162.0
89	162.0
90	124.0
91	86.0
92	57.0
93	28.0
94	23.5
95	19.0
96	19.0
97	12.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1139534.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.62778637101084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.1351705503173	62.55806765759028
2	8.422787253907801	12.234567866505776
3	2.3467829529687165	5.1132495290202575
4	1.0173861747133133	2.9556202301559367
5	0.5143315303792356	1.8677380256129097
6	0.3579963782792565	1.5600290689956853
7	0.23574033322981214	1.198490899259199
8	0.1717540512372738	0.9979293233293097
9	0.119696360475029	0.7823953528171121
>10	0.6471332332726142	8.30518383873951
>50	0.026491287354131445	1.2738213488399268
>100	0.004002222453314927	0.40984761505484
>500	2.42557137405612E-4	0.1064029832678826
>1k	4.85114274811224E-4	0.6366562608114698
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2214	0.19428994659220347	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2045	0.17945932284600546	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1707	0.14979807535360945	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1262	0.1107470246609579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.755103401916924E-4	0.0
11	0.0	0.0	0.0	2.6326551028753856E-4	0.0
12	0.0	0.0	0.0	4.3877585047923096E-4	0.0
13	0.0	0.0	0.0	4.3877585047923096E-4	0.0
14	0.0	0.0	0.0	4.3877585047923096E-4	0.0
15	0.0	0.0	0.0	5.265310205750771E-4	0.0
16	0.0	0.0	0.0	5.265310205750771E-4	0.0
17	0.0	0.0	0.0	6.142861906709234E-4	0.0
18	0.0	0.0	0.0	6.142861906709234E-4	0.0
19	0.0	0.0	0.0	7.020413607667696E-4	0.0
20	0.0	0.0	0.0	0.0010530620411501543	0.0
21	0.0	0.0	0.0	0.0016673482318210777	0.0
22	0.0	0.0	0.0	0.0021061240823003085	0.0
23	0.0	0.0	0.0	0.0025448999327795396	0.0
24	0.0	0.0	0.0	0.004212248164600617	0.0
25	0.0	0.0	0.0	0.006142861906709234	0.0
26	0.0	0.0	0.0	0.010091844561022313	0.0
27	0.0	0.0	0.0	0.05116126416587833	0.0
28	0.0	0.0	0.0	0.13593275847846575	0.0
29	0.0	0.0	0.0	0.23132262837265058	0.0
30	0.0	0.0	0.0	0.3407533254821708	0.0
31	0.0	0.0	0.0	0.5576841059591026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	595	0.0	26.428572	1
GCGGTTA	45	0.0038257872	20.555557	32
TCGTATT	50	0.007034846	18.5	24
TAGCACA	165	3.6379788E-12	17.939394	4
GTATCAA	890	0.0	17.252808	2
CTAGCCT	135	2.2189852E-8	16.444445	4
TGCGCAA	70	0.0025924745	15.857143	10
CGCACGA	70	0.0025924745	15.857143	28
TATAGAC	85	5.3640996E-4	15.235294	3
GTTCTAC	140	5.9941885E-7	14.535715	1
TAAGCTA	130	4.443589E-6	14.230769	33
AGCGATA	80	0.0062985774	13.875	22
GGTTAGA	80	0.0062985774	13.875	1
TGTACTG	285	0.0	13.631578	5
GTCTAAT	110	2.4563298E-4	13.454545	1
TCAACGC	1190	0.0	13.369748	5
TAGGGTC	125	4.8443093E-5	13.32	5
TACACTT	335	0.0	13.253732	5
TAATACT	295	0.0	13.169492	4
GTATATA	225	4.0563464E-10	13.155556	1
>>END_MODULE