FastQCFastQC Report
Fri 10 Feb 2017
ERR1630047.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630047.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170822
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68190.3141206418582454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64090.29523378701708386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49410.22760963358580297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.15832712216846892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.027.2805961
CGAATTA2650.018.15094215
GTATCAA28100.016.2615662
GCTTAGG5850.015.1794861
CCGAATT3450.015.01449314
CGACCAA2006.184564E-1114.812
CGTGCGA1651.6472768E-814.57575710
GTACTAG1406.001428E-714.5357141
TAGGACA7400.014.2500014
TTAGGAC7100.014.0704233
TGTCCGA4900.013.96938712
CTAGCAC5050.013.9207933
GTCCTAT1907.1358954E-913.6315791
ACGCAAT2750.013.45454511
TACGCAT1102.4579375E-413.45454528
TAGAACA6200.013.427424
GCTCTAT1254.8482565E-513.3200011
TCGATTG850.0094082513.0588248
TCGTACT850.0094082513.0588244
GTTCTAG7300.012.9246581
Produced by FastQC (version 0.11.5)