##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630047.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2170822
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82967419714744	34.0	31.0	34.0	31.0	34.0
2	33.05692682311125	34.0	33.0	34.0	31.0	34.0
3	33.183019151270805	34.0	33.0	34.0	31.0	34.0
4	36.47890338314242	37.0	37.0	37.0	35.0	37.0
5	36.422170956439544	37.0	37.0	37.0	35.0	37.0
6	36.46773019621139	37.0	37.0	37.0	35.0	37.0
7	36.40058512397608	37.0	37.0	37.0	35.0	37.0
8	36.40481071225554	37.0	37.0	37.0	35.0	37.0
9	38.17702556911622	39.0	39.0	39.0	37.0	39.0
10	38.152469433237734	39.0	39.0	39.0	37.0	39.0
11	38.18971753556948	39.0	39.0	39.0	37.0	39.0
12	38.127037592211614	39.0	39.0	39.0	37.0	39.0
13	38.13796386806472	39.0	39.0	39.0	37.0	39.0
14	39.5874558116695	41.0	40.0	41.0	37.0	41.0
15	39.5746376257473	41.0	40.0	41.0	37.0	41.0
16	39.48869736901506	41.0	39.0	41.0	37.0	41.0
17	39.421709840788424	41.0	39.0	41.0	37.0	41.0
18	39.31572049665979	41.0	39.0	41.0	36.0	41.0
19	39.34428294903958	41.0	39.0	41.0	36.0	41.0
20	39.56422728348985	41.0	39.0	41.0	37.0	41.0
21	39.57076259591989	41.0	40.0	41.0	37.0	41.0
22	39.59028054810574	41.0	40.0	41.0	37.0	41.0
23	39.524013944948045	41.0	40.0	41.0	37.0	41.0
24	39.48972739358639	41.0	40.0	41.0	37.0	41.0
25	39.46274084194835	41.0	40.0	41.0	37.0	41.0
26	39.318658093570086	41.0	39.0	41.0	36.0	41.0
27	39.23789375637431	41.0	39.0	41.0	36.0	41.0
28	39.195482632845994	41.0	39.0	41.0	36.0	41.0
29	39.08851946405555	41.0	39.0	41.0	36.0	41.0
30	39.00500870177288	40.0	39.0	41.0	35.0	41.0
31	38.874498231545466	40.0	39.0	41.0	35.0	41.0
32	38.78538267992493	40.0	39.0	41.0	35.0	41.0
33	38.71353754476415	40.0	38.0	41.0	35.0	41.0
34	38.63922836602909	40.0	38.0	41.0	35.0	41.0
35	38.499887139525946	40.0	38.0	41.0	35.0	41.0
36	38.49159765287067	40.0	38.0	41.0	35.0	41.0
37	38.44939106016062	40.0	38.0	41.0	35.0	41.0
38	38.34773141234058	40.0	38.0	41.0	34.0	41.0
39	38.29617859041414	40.0	38.0	41.0	34.0	41.0
40	38.24215711836346	40.0	38.0	41.0	34.0	41.0
41	38.13711902680183	40.0	38.0	41.0	34.0	41.0
42	38.05061308573434	40.0	38.0	41.0	34.0	41.0
43	37.75069213413168	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	4.0
15	10.0
16	9.0
17	46.0
18	102.0
19	152.0
20	256.0
21	465.0
22	919.0
23	1459.0
24	2433.0
25	3515.0
26	5054.0
27	7024.0
28	9462.0
29	12844.0
30	16464.0
31	21690.0
32	27707.0
33	36372.0
34	49183.0
35	69314.0
36	104592.0
37	184872.0
38	399922.0
39	1216947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19285505674809	19.267632260959214	12.262221407374717	25.277291274917978
2	18.45061455983033	21.552987762239372	35.43491820149234	24.56147947643796
3	18.928037397815206	23.462402721181196	31.36949966418251	26.240060216821092
4	14.281778975890239	16.054978252477632	36.099459098903544	33.56378367272858
5	13.802283190422798	36.92131367749175	35.45845767179437	13.817945460291078
6	33.23487600549469	36.36129539870151	15.6311295905422	14.772699005261602
7	27.784774615330043	31.15695344897002	22.44324960775227	18.61502232794766
8	25.932849399904732	34.75816994668379	20.498041755611467	18.810938897800003
9	25.59362306075763	14.757727717887509	20.607032727694854	39.04161649366
10	16.22905977551361	27.394369506113353	34.80151758181923	21.575053136553805
11	33.969344331317814	22.579879879603208	22.694214449641656	20.75656133943732
12	19.947144445744517	26.0318902240718	31.019954653122184	23.0010106770615
13	29.23537719813048	20.796730455099496	25.854584116062945	24.11330823070708
14	21.565471512634385	21.78584886278101	26.527186475906362	30.12149314867824
15	23.989760560746117	28.70373526710159	23.56218059334206	23.74432357881024
16	23.45429519324938	27.759116132045836	25.24048494072752	23.546103733977265
17	22.50990638569169	27.638240261062396	26.871249692512787	22.980603660733124
18	22.397230173639294	26.757421842970082	27.706232938490583	23.139115044900045
19	23.547485698965644	26.345780538431985	28.122572923989164	21.984160838613207
20	23.575078933233588	26.176443761856106	27.850417952277983	22.39805935263232
21	22.772157274986156	26.768846086874003	27.151373995656947	23.307622642482894
22	23.04550994968726	26.413727150360554	27.36723692684154	23.173525973110646
23	23.06573270401719	26.511432075038854	27.397916549583524	23.024918671360435
24	23.206370674334423	26.551831518199098	27.353325145958536	22.888472661507944
25	23.500452823861192	26.025394988626427	27.492120496291268	22.982031691221113
26	22.90851115383942	26.788377858709744	27.332825998630934	22.970284988819902
27	23.02731407734029	26.81288470450364	26.908102092202864	23.25169912595321
28	22.84963944533453	26.739133839623886	27.488757714819545	22.922469000222033
29	22.95706419043109	26.85208644467395	27.515798163092136	22.67505120180282
30	22.8542920607954	27.189055574340042	27.27418461762411	22.682467747240445
31	23.02487260586082	26.588637852389557	27.544128445353877	22.842361096395745
32	22.470197925025637	26.756638729476666	27.659061866887292	23.114101478610408
33	22.591949040501706	26.581866223946506	27.69305820560138	23.133126529950406
34	22.882069557061797	26.076205234699113	27.69913885155024	23.342586356688848
35	22.82522473053986	26.283776375953437	28.01777391237052	22.87322498113618
36	22.69978837509478	25.998078147356164	28.042418954663255	23.2597145228858
37	22.84963944533453	24.914801858466518	28.45968025015409	23.775878446044864
38	22.33342945667586	25.451004273957057	29.0342091613223	23.181357108044786
39	22.02958142123122	25.63674036839501	29.07847810645	23.25520010392377
40	21.798885399171375	25.023977092548357	29.64499162068562	23.532145887594652
41	21.38231508617473	25.081144377567576	29.84901571846978	23.687524817787917
42	21.308518155795362	24.95506310512792	29.66475372001942	24.0716650190573
43	21.016739281249222	25.00172745623547	29.920509373868516	24.06102388864679
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1637.0
1	1358.0
2	1079.0
3	2029.0
4	2979.0
5	2979.0
6	3316.5
7	3654.0
8	3110.5
9	2567.0
10	3682.5
11	4798.0
12	4798.0
13	7473.0
14	10148.0
15	13659.0
16	17170.0
17	17880.5
18	18591.0
19	18591.0
20	23150.0
21	27709.0
22	29041.0
23	30373.0
24	35504.5
25	40636.0
26	40636.0
27	44618.0
28	48600.0
29	57426.0
30	66252.0
31	73521.0
32	80790.0
33	80790.0
34	88150.5
35	95511.0
36	104446.5
37	113382.0
38	118758.5
39	124135.0
40	124135.0
41	131424.5
42	138714.0
43	137151.0
44	135588.0
45	138872.0
46	142156.0
47	142156.0
48	142272.0
49	142388.0
50	146896.0
51	151404.0
52	153566.0
53	155728.0
54	155728.0
55	143814.0
56	131900.0
57	123029.0
58	114158.0
59	103786.5
60	93415.0
61	93415.0
62	86095.0
63	78775.0
64	70317.5
65	61860.0
66	53607.0
67	45354.0
68	45354.0
69	38684.5
70	32015.0
71	27492.0
72	22969.0
73	18804.0
74	14639.0
75	14639.0
76	11747.0
77	8855.0
78	7163.5
79	5472.0
80	4099.5
81	2727.0
82	2727.0
83	2054.0
84	1381.0
85	1030.5
86	680.0
87	513.0
88	346.0
89	346.0
90	262.0
91	178.0
92	118.0
93	58.0
94	40.0
95	22.0
96	22.0
97	19.0
98	16.0
99	14.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2170822.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.8245100093599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53363373118303	46.33108786057093
2	10.749688302475032	12.21691541082983
3	3.219453312385649	5.488315709229756
4	1.370057037023904	3.114112792550352
5	0.7490258074622954	2.128151224670504
6	0.4771619814652849	1.626869747511605
7	0.3334700885742681	1.3264492070207439
8	0.22739636566878746	1.0337349645630463
9	0.18263153742940597	0.934015286401386
>10	0.9860793130595664	10.283375203440235
>50	0.09062102967955936	3.5993435331595167
>100	0.07416773769555589	8.17387084013172
>500	0.0055522889016268095	2.2097026227407786
>1k	8.98164381145508E-4	0.9203034440328223
>5k	1.6330261475372873E-4	0.6137521531467695
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6819	0.3141206418582454	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6409	0.29523378701708386	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4941	0.22760963358580297	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3437	0.15832712216846892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.606549961258915E-5	0.0
11	0.0	0.0	0.0	4.606549961258915E-5	0.0
12	0.0	0.0	0.0	4.606549961258915E-5	0.0
13	0.0	0.0	0.0	4.606549961258915E-5	0.0
14	0.0	0.0	0.0	4.606549961258915E-5	0.0
15	0.0	0.0	0.0	1.3819649883776744E-4	0.0
16	0.0	0.0	0.0	1.3819649883776744E-4	0.0
17	0.0	0.0	0.0	1.842619984503566E-4	0.0
18	0.0	0.0	0.0	2.763929976755349E-4	0.0
19	0.0	0.0	0.0	3.685239969007132E-4	0.0
20	0.0	0.0	0.0	5.988514949636589E-4	0.0
21	0.0	0.0	0.0	7.831134934140155E-4	0.0
22	0.0	0.0	0.0	0.001520161487215442	0.0
23	0.0	0.0	0.0	0.0027178644771427597	0.0
24	0.0	0.0	0.0	0.005712121951961055	0.0
25	0.0	0.0	0.0	0.010134409914769613	0.0
26	0.0	0.0	0.0	0.01847226534464825	0.0
27	0.0	0.0	0.0	0.07241496539099014	0.0
28	0.0	0.0	0.0	0.16781661508866227	0.0
29	0.0	0.0	0.0	0.28081528563834346	0.0
30	0.0	0.0	0.0	0.40777180257063916	0.0
31	0.0	0.0	0.0	0.6613163124383298	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1675	0.0	27.280596	1
CGAATTA	265	0.0	18.150942	15
GTATCAA	2810	0.0	16.261566	2
GCTTAGG	585	0.0	15.179486	1
CCGAATT	345	0.0	15.014493	14
CGACCAA	200	6.184564E-11	14.8	12
CGTGCGA	165	1.6472768E-8	14.575757	10
GTACTAG	140	6.001428E-7	14.535714	1
TAGGACA	740	0.0	14.250001	4
TTAGGAC	710	0.0	14.070423	3
TGTCCGA	490	0.0	13.969387	12
CTAGCAC	505	0.0	13.920793	3
GTCCTAT	190	7.1358954E-9	13.631579	1
ACGCAAT	275	0.0	13.454545	11
TACGCAT	110	2.4579375E-4	13.454545	28
TAGAACA	620	0.0	13.42742	4
GCTCTAT	125	4.8482565E-5	13.320001	1
TCGATTG	85	0.00940825	13.058824	8
TCGTACT	85	0.00940825	13.058824	4
GTTCTAG	730	0.0	12.924658	1
>>END_MODULE