FastQCFastQC Report
Fri 10 Feb 2017
ERR1630046.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630046.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334260
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46581.3935259977263208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38481.1511996649314904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29300.8765631544306827No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.5908574163824567No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.1723209477652127No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.16663674983545743No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.1633458984024412No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.16065338359360978No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.14509663136480583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATAA250.005490837629.5999988
TAGACTG405.9277234E-527.7500025
GTGTATC555.132614E-420.1818181
GTTCGCT500.007024703518.4999989
GATGCTA500.007024703518.49999814
AGTTCGC500.007024703518.4999988
AGTCTTA752.0617455E-417.26666627
ACTGTGG650.001576766717.0769238
GGTATCA16600.016.382531
CTAGAGG700.002587339615.85714218
TAATACT700.002587339615.8571424
ATACTGG700.002587339615.8571426
CAGTCAA700.002587339615.85714215
CTTGCTC1201.9254276E-615.4166673
CCCCTTG855.350029E-415.2352947
AGTCAAA750.00409631414.816
TATAGTA1001.08968365E-414.7999992
TATACAC2201.8189894E-1114.29545437
GAGTACA800.00628630513.8750011
AATACTG950.001241843313.6315795
Produced by FastQC (version 0.11.5)