##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630046.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334260
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72698797343386	34.0	31.0	34.0	31.0	34.0
2	32.93406031233172	34.0	31.0	34.0	31.0	34.0
3	33.05337162687728	34.0	33.0	34.0	31.0	34.0
4	36.32571949979058	37.0	37.0	37.0	35.0	37.0
5	36.320008376712735	37.0	37.0	37.0	35.0	37.0
6	36.40784419314306	37.0	37.0	37.0	35.0	37.0
7	36.32250044875247	37.0	37.0	37.0	35.0	37.0
8	36.34343325554957	37.0	37.0	37.0	35.0	37.0
9	38.10449949141387	39.0	39.0	39.0	37.0	39.0
10	38.102866032429844	39.0	39.0	39.0	37.0	39.0
11	38.13595703943038	39.0	39.0	39.0	37.0	39.0
12	38.076090468497576	39.0	39.0	39.0	37.0	39.0
13	38.10235744629929	39.0	39.0	39.0	37.0	39.0
14	39.45322204272124	41.0	39.0	41.0	37.0	41.0
15	39.43720756297493	41.0	39.0	41.0	37.0	41.0
16	39.35189672709867	41.0	39.0	41.0	36.0	41.0
17	39.34751989469275	41.0	39.0	41.0	36.0	41.0
18	39.29717585113385	41.0	39.0	41.0	36.0	41.0
19	39.34774726260994	41.0	39.0	41.0	36.0	41.0
20	39.552539938969666	41.0	39.0	41.0	37.0	41.0
21	39.53359360976486	41.0	40.0	41.0	37.0	41.0
22	39.50241129659546	41.0	39.0	41.0	37.0	41.0
23	39.41394124334351	41.0	39.0	41.0	36.0	41.0
24	39.38315084066296	41.0	39.0	41.0	36.0	41.0
25	39.32230299766649	41.0	39.0	41.0	36.0	41.0
26	39.13800933405134	41.0	39.0	41.0	35.0	41.0
27	38.975949859390894	41.0	39.0	41.0	35.0	41.0
28	38.91591874588644	41.0	39.0	41.0	35.0	41.0
29	38.739307724525816	40.0	38.0	41.0	35.0	41.0
30	38.56433913719859	40.0	38.0	41.0	35.0	41.0
31	38.3305690181296	40.0	38.0	41.0	35.0	41.0
32	38.15388021300784	40.0	37.0	41.0	35.0	41.0
33	38.00825704541375	40.0	37.0	41.0	34.0	41.0
34	37.87513911326512	40.0	37.0	41.0	34.0	41.0
35	37.66102136061748	40.0	37.0	41.0	33.0	41.0
36	37.53702806198768	40.0	36.0	41.0	33.0	41.0
37	37.406106025249805	40.0	36.0	41.0	33.0	41.0
38	37.19419015137917	40.0	36.0	41.0	33.0	41.0
39	37.04581164363071	40.0	36.0	41.0	32.0	41.0
40	36.85283013223239	40.0	35.0	41.0	31.0	41.0
41	36.622554299048645	40.0	35.0	41.0	30.0	41.0
42	36.3875456231676	40.0	35.0	41.0	29.0	41.0
43	35.97148626817447	40.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	3.0
16	5.0
17	8.0
18	9.0
19	24.0
20	46.0
21	77.0
22	161.0
23	287.0
24	495.0
25	777.0
26	1127.0
27	1728.0
28	2359.0
29	3126.0
30	3955.0
31	4713.0
32	5852.0
33	7588.0
34	10255.0
35	13217.0
36	19132.0
37	38790.0
38	59785.0
39	160739.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.535810446957456	19.44534194938072	12.731107521091367	22.287740082570455
2	19.959013941243345	23.531981092562678	33.69024112965955	22.818763836534433
3	21.021061449171306	23.76473403937055	30.906779153952012	24.307425357506133
4	13.901753126308861	16.065338359360975	35.34733441033926	34.68557410399091
5	13.968467659905462	37.380183091007005	33.850296176628966	14.801053072458565
6	33.145754801651414	35.799377729910844	14.171602943816191	16.883264524621552
7	26.95955244420511	31.24184766349548	21.8500568419793	19.94854305032011
8	26.836893436247234	33.071860228564596	20.17950098725543	19.911745347932747
9	24.90606115000299	13.788966672650032	21.610123855681206	39.69484832166577
10	17.329324477951296	27.62161191886555	33.0126249027703	22.03643870041285
11	32.53006641536528	22.87590498414408	21.419254472566266	23.17477412792437
12	20.81194279901873	28.545742834918926	29.72147430144199	20.920840064620354
13	31.0838867947107	20.693472147430146	24.71010590558248	23.51253515227667
14	21.086280141207446	23.42457966852151	28.624124932687128	26.86501525758392
15	24.497696403996887	28.33542751151798	25.60372165380243	21.5631544306827
16	21.343564889606892	27.83073056901813	26.890145395799674	23.9355591455753
17	20.56243642673368	27.537246454855502	28.37312271884162	23.527194399569197
18	20.78232513612158	26.085382636271166	29.911446179620654	23.220846047986598
19	22.279064201519773	25.911865015257586	31.00191467719739	20.80715610602525
20	22.95608209178484	26.649614072877405	30.517860348231917	19.876443487105846
21	22.139352599772632	27.02237778974451	29.520134027403817	21.31813558307904
22	20.949560222581223	27.521091366002516	30.758690839466286	20.77065757194998
23	21.357924968587326	27.080715610602525	29.904266140130435	21.65709328067971
24	21.66696583497876	27.556393226829414	30.041584395380845	20.735056542810987
25	21.357625800275233	27.56327409800754	29.713097588703402	21.36600251301382
26	20.853527194399568	27.895650092742173	30.158559205408963	21.092263507449292
27	20.720995632142643	27.570154969185662	30.50709028899659	21.201759109675102
28	20.63872434631724	27.8358164303237	30.262968946329206	21.26249027702986
29	20.485550170525936	27.94082450786813	30.866989768443727	20.706635553162208
30	20.92532758930174	28.49249087536648	30.294082450786814	20.288099084544964
31	20.931011787231498	28.978041045892418	30.145096631364805	19.945850535511276
32	19.790881349847425	28.434153054508464	30.56782145635134	21.207144139292765
33	19.804942260515766	28.10207622808592	30.79070184886017	21.302279662538144
34	19.748100281218214	27.061269670316516	31.855741039909052	21.334889008556214
35	19.514449829474064	27.740680907078325	30.86639143181954	21.878477831628075
36	20.457727517501347	28.400047866929935	30.314126727697005	20.828097887871717
37	20.044576078501766	27.640459522527372	30.517561179919824	21.797403219051038
38	19.95482558487405	27.542033147848983	31.22958176269969	21.273559504577275
39	19.14617363728834	27.39035481361814	31.912283850894514	21.551187698199005
40	18.737509722970145	27.143840124454016	32.35475378447914	21.76389636809669
41	18.808113444623945	26.7674863878418	32.47352360437982	21.95087656315443
42	18.295937294321785	27.03374618560402	32.24196733082032	22.428349189253876
43	18.308203195117574	26.380063423682167	32.299108478429964	23.0126249027703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	300.0
1	280.0
2	260.0
3	625.5
4	991.0
5	991.0
6	1268.5
7	1546.0
8	1459.0
9	1372.0
10	1812.5
11	2253.0
12	2253.0
13	4045.0
14	5837.0
15	8144.5
16	10452.0
17	9165.5
18	7879.0
19	7879.0
20	8534.0
21	9189.0
22	7291.5
23	5394.0
24	5783.5
25	6173.0
26	6173.0
27	6850.5
28	7528.0
29	8627.5
30	9727.0
31	10809.0
32	11891.0
33	11891.0
34	12947.5
35	14004.0
36	15004.0
37	16004.0
38	16521.5
39	17039.0
40	17039.0
41	17191.0
42	17343.0
43	17939.0
44	18535.0
45	17974.0
46	17413.0
47	17413.0
48	17732.5
49	18052.0
50	18524.5
51	18997.0
52	18977.0
53	18957.0
54	18957.0
55	19200.5
56	19444.0
57	19380.0
58	19316.0
59	18326.5
60	17337.0
61	17337.0
62	15635.5
63	13934.0
64	12213.0
65	10492.0
66	8710.5
67	6929.0
68	6929.0
69	5609.5
70	4290.0
71	3468.0
72	2646.0
73	2014.0
74	1382.0
75	1382.0
76	1042.0
77	702.0
78	517.0
79	332.0
80	248.5
81	165.0
82	165.0
83	120.5
84	76.0
85	59.0
86	42.0
87	30.5
88	19.0
89	19.0
90	14.5
91	10.0
92	5.5
93	1.0
94	2.5
95	4.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	334260.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.48965660954552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.3677757518047	60.175451982653996
2	10.167694063068746	14.33434526033261
3	2.390871998355879	5.055952384844514
4	0.7625090423079431	2.149960022158413
5	0.3272287911120231	1.1533122559121602
6	0.18385879409897232	0.7776085956409016
7	0.11752032434702975	0.5798777115505176
8	0.09240733290295253	0.5211008931626465
9	0.06960096526829686	0.44155333273096353
>10	0.45440330876002843	6.252406607769088
>50	0.048210632006482956	2.3153896939030116
>100	0.014505853878031438	1.5433926365884032
>500	0.0017065710444742867	0.666437662252514
>1k	0.0017065710444742867	4.0332109605002096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4658	1.3935259977263208	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3848	1.1511996649314904	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2930	0.8765631544306827	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1975	0.5908574163824567	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	576	0.1723209477652127	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.16663674983545743	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.1633458984024412	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.16065338359360978	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	485	0.14509663136480583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	2.991683120923832E-4	0.0
15	0.0	0.0	0.0	2.991683120923832E-4	0.0
16	0.0	0.0	0.0	2.991683120923832E-4	0.0
17	0.0	0.0	0.0	8.975049362771495E-4	0.0
18	0.0	0.0	0.0	0.0011966732483695327	0.0
19	0.0	0.0	0.0	0.0011966732483695327	0.0
20	0.0	0.0	0.0	0.001495841560461916	0.0
21	0.0	0.0	0.0	0.0020941781846466824	0.0
22	0.0	0.0	0.0	0.005085861305570514	0.0
23	0.0	0.0	0.0	0.005983366241847664	0.0
24	0.0	0.0	0.0	0.013462574044157244	0.0
25	0.0	0.0	0.0	0.024232633279483037	0.0
26	0.0	0.0	0.0	0.043080236941303175	0.0
27	0.0	0.0	0.0	0.14360078980434393	0.0
28	0.0	0.0	0.0	0.3293843116137139	0.0
29	0.0	0.0	0.0	0.5818823670196853	0.0
30	0.0	0.0	0.0	0.8586130557051397	0.0
31	0.0	0.0	0.0	1.249326871297792	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCATAA	25	0.0054908376	29.599998	8
TAGACTG	40	5.9277234E-5	27.750002	5
GTGTATC	55	5.132614E-4	20.181818	1
GTTCGCT	50	0.0070247035	18.499998	9
GATGCTA	50	0.0070247035	18.499998	14
AGTTCGC	50	0.0070247035	18.499998	8
AGTCTTA	75	2.0617455E-4	17.266666	27
ACTGTGG	65	0.0015767667	17.076923	8
GGTATCA	1660	0.0	16.38253	1
CTAGAGG	70	0.0025873396	15.857142	18
TAATACT	70	0.0025873396	15.857142	4
ATACTGG	70	0.0025873396	15.857142	6
CAGTCAA	70	0.0025873396	15.857142	15
CTTGCTC	120	1.9254276E-6	15.416667	3
CCCCTTG	85	5.350029E-4	15.235294	7
AGTCAAA	75	0.004096314	14.8	16
TATAGTA	100	1.08968365E-4	14.799999	2
TATACAC	220	1.8189894E-11	14.295454	37
GAGTACA	80	0.006286305	13.875001	1
AATACTG	95	0.0012418433	13.631579	5
>>END_MODULE