FastQCFastQC Report
Fri 10 Feb 2017
ERR1630045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51641
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14982.900795879243237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12922.5018880347011097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8731.6905172246858118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5561.0766638910942856No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.32338645649774406No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.2943397687883658No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.2691659727735714No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.26529308107898764No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.25561085184252824No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.25367440599523633No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA1230.23818283921690128No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.22269127243856626No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT880.1704072345616855No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTGTCT820.1587885594779342No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA760.1471698843941829No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG670.12974187176855598No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.12974187176855598No Hit
CAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.12005964253209658No Hit
GCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.11618675083751283No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACCTGTC580.11231385914292906No Hit
GTGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTC570.11037741329563719No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.10650452160105342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4750.021.0315781
GCCTGTA609.074896E-418.52
CCTGTAC650.001552463217.0769233
TGCCTGT650.001552463217.0769231
TGTACTG803.3049413E-416.18755
CCTCCCC750.00403420614.812
CTGTACT750.00403420614.84
ACTGCCT908.0868404E-414.3888888
GTATCAA7000.014.2714282
GTACTGC800.006191781313.8756
TTATACA800.006191781313.87537
TGCCTCC800.006191781313.87510
GGGGGAG1652.4362453E-713.45454519
CTAAAAA1254.6716865E-513.3210
GCCTCCC850.0092465213.05882411
TACTGCC850.0092465213.0588247
CTTATAC1153.4751816E-412.86956437
TCTCTTA2652.7284841E-1112.56603837
AACGCAG9700.012.2061855
ACGGGGG1552.6037114E-511.93548317
Produced by FastQC (version 0.11.5)