##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51641
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.551538506225675	33.0	31.0	34.0	31.0	34.0
2	32.72564435235569	34.0	31.0	34.0	31.0	34.0
3	32.798687089715536	34.0	31.0	34.0	31.0	34.0
4	36.12391316976821	37.0	35.0	37.0	35.0	37.0
5	36.17040723456169	37.0	35.0	37.0	35.0	37.0
6	36.30706221800507	37.0	37.0	37.0	35.0	37.0
7	36.20202939524796	37.0	37.0	37.0	35.0	37.0
8	36.260316415251445	37.0	37.0	37.0	35.0	37.0
9	37.950814275478784	39.0	38.0	39.0	35.0	39.0
10	37.95522937201061	39.0	38.0	39.0	35.0	39.0
11	38.00356306035901	39.0	38.0	39.0	35.0	39.0
12	37.92289072636084	39.0	38.0	39.0	35.0	39.0
13	37.949749230262775	39.0	38.0	39.0	35.0	39.0
14	39.16608896032223	40.0	39.0	41.0	36.0	41.0
15	39.17120117735907	40.0	39.0	41.0	36.0	41.0
16	38.981991053620185	40.0	38.0	41.0	36.0	41.0
17	39.101934509401445	40.0	39.0	41.0	36.0	41.0
18	39.06723339981797	40.0	39.0	41.0	36.0	41.0
19	39.12387444085126	40.0	39.0	41.0	36.0	41.0
20	39.30721713367286	41.0	39.0	41.0	36.0	41.0
21	39.26111035804884	41.0	39.0	41.0	36.0	41.0
22	39.19500009682229	41.0	39.0	41.0	35.0	41.0
23	39.08638484924769	41.0	39.0	41.0	35.0	41.0
24	39.00152979221936	41.0	39.0	41.0	35.0	41.0
25	38.940841579365234	41.0	38.0	41.0	35.0	41.0
26	38.62134737902055	40.0	38.0	41.0	35.0	41.0
27	38.37051954842083	40.0	37.0	41.0	35.0	41.0
28	38.192579539513176	40.0	37.0	41.0	34.0	41.0
29	37.936426482833404	40.0	37.0	41.0	34.0	41.0
30	37.64309366588564	40.0	36.0	41.0	33.0	41.0
31	37.28578067814333	39.0	35.0	41.0	33.0	41.0
32	36.958017854030714	39.0	35.0	41.0	33.0	41.0
33	36.71267016517883	39.0	35.0	41.0	33.0	41.0
34	36.45398036443911	39.0	35.0	41.0	32.0	41.0
35	36.11992409132279	38.0	35.0	41.0	31.0	41.0
36	35.80734300265293	38.0	35.0	41.0	30.0	41.0
37	35.5177668906489	38.0	35.0	41.0	28.0	41.0
38	35.176778141399275	38.0	35.0	41.0	25.0	41.0
39	34.82885691601634	38.0	35.0	41.0	23.0	41.0
40	34.406595534555876	37.0	35.0	40.0	20.0	41.0
41	33.942816754129474	37.0	34.0	40.0	16.0	41.0
42	33.44100617726225	37.0	33.0	40.0	12.0	41.0
43	32.84336089541256	36.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	2.0
20	15.0
21	31.0
22	43.0
23	75.0
24	141.0
25	205.0
26	295.0
27	420.0
28	636.0
29	771.0
30	953.0
31	1150.0
32	1356.0
33	1722.0
34	2263.0
35	2786.0
36	4020.0
37	8929.0
38	9918.0
39	15907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.38324199763753	17.02329544354292	14.418775778935341	19.1746867798842
2	21.527468484343835	24.07583121937995	31.90294533413373	22.493754962142486
3	22.97205708642358	24.71292190313898	31.577622431788694	20.737398578648747
4	14.269669448693866	17.141418640227727	30.930849518793206	37.6580623922852
5	13.64419744001859	38.51203501094092	31.914564009217482	15.929203539823009
6	33.14808001394241	32.91377006642009	13.351794117077517	20.58635580255998
7	26.53318099959335	31.55051218992661	20.780000387289167	21.136306423190877
8	29.823202494142254	31.420770318158052	17.91793342499177	20.838093762707928
9	25.745047539745553	13.671307681880677	19.35090335198776	41.23274142638601
10	21.196336244456923	27.67568404949556	26.5680370248446	24.55994268120292
11	34.689490908386745	21.736604635851357	19.445789198505064	24.12811525725683
12	22.137448926240776	30.224046784531673	26.645494858736274	20.993009430491277
13	34.222807459189404	19.41093317325381	24.110687244631205	22.25557212292558
14	21.11306907302337	24.07583121937995	29.290679886136985	25.520419821459694
15	26.353091535795205	27.820917488042447	24.604480935690635	21.22151004047172
16	20.07707054472222	26.19817586801185	29.058306384461957	24.666447202803973
17	21.115005518870664	28.61486028543212	27.882883755155785	22.387250440541433
18	21.24668383648651	23.357409810034664	29.889041652950176	25.50686470052865
19	23.97126314362619	24.74584148254294	32.30378962452315	18.97910574930772
20	23.268333301059236	24.604480935690635	31.65508026568037	20.47210549756976
21	22.69127243856626	25.04405414302589	32.39092968765129	19.87374373075657
22	21.765651323560736	26.35696442748979	32.2689335992719	19.608450649677582
23	20.87488623380647	26.01034062082454	31.858407079646017	21.256366065722972
24	20.72771634941229	26.781046068046706	32.42965860459712	20.06157897794388
25	20.46048682248601	26.15363761352414	32.25344203249356	21.132433531496293
26	19.989930481594083	27.088940957766116	33.151952905636996	19.769175655002808
27	20.03059584438721	27.220619275381964	33.10935109699657	19.639433783234253
28	19.2695726264015	28.70974613194942	32.710443252454446	19.310237989194633
29	19.683972037721965	28.653589202377955	32.42772215874983	19.234716601150247
30	18.818380743982495	28.887899149900274	33.20036405181929	19.093356054297942
31	18.760287368563738	28.616796731279408	32.85761313684863	19.765302763308224
32	19.01783466625356	28.6903816734765	32.803392653124455	19.488391007145488
33	18.764160260258322	29.220967835634475	31.90488177998102	20.109990124126178
34	18.87647411940125	28.616796731279408	32.17404775275459	20.332681396564745
35	18.713812668228734	28.477372630274395	32.78402819465154	20.024786506845334
36	18.518231637652253	27.981642493367676	32.64654053949381	20.85358532948626
37	17.941170775159275	28.045545206328303	32.91377006642009	21.09951395209233
38	17.753335527971963	27.9274220096435	33.471466470440156	20.847775991944385
39	17.985709029646983	27.75507832923452	34.01173486183458	20.2474777792839
40	17.809492457543424	28.343757866811252	33.53536918340079	20.311380492244535
41	17.530644255533396	27.880947309308496	33.721267984740805	20.867140450417303
42	16.56823066942933	28.130748823609146	32.99510079200635	22.305919714955174
43	16.73476501229643	27.880947309308496	33.0551306132724	22.329157065122672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	98.0
2	102.0
3	200.0
4	298.0
5	298.0
6	363.0
7	428.0
8	416.5
9	405.0
10	539.0
11	673.0
12	673.0
13	1208.5
14	1744.0
15	2451.0
16	3158.0
17	2749.0
18	2340.0
19	2340.0
20	2477.0
21	2614.0
22	1794.0
23	974.0
24	836.5
25	699.0
26	699.0
27	665.0
28	631.0
29	675.5
30	720.0
31	821.0
32	922.0
33	922.0
34	951.0
35	980.0
36	1070.5
37	1161.0
38	1250.0
39	1339.0
40	1339.0
41	1386.5
42	1434.0
43	1563.5
44	1693.0
45	1907.0
46	2121.0
47	2121.0
48	2446.0
49	2771.0
50	2929.5
51	3088.0
52	3093.0
53	3098.0
54	3098.0
55	3122.5
56	3147.0
57	3143.5
58	3140.0
59	2979.0
60	2818.0
61	2818.0
62	2635.0
63	2452.0
64	2228.0
65	2004.0
66	1793.5
67	1583.0
68	1583.0
69	1360.5
70	1138.0
71	969.5
72	801.0
73	645.0
74	489.0
75	489.0
76	382.5
77	276.0
78	210.5
79	145.0
80	117.0
81	89.0
82	89.0
83	68.0
84	47.0
85	29.5
86	12.0
87	11.5
88	11.0
89	11.0
90	6.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	51641.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.26587401483317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.01741718569144	61.84620747080808
2	8.14914843190211	11.45214074088418
3	1.8740009921181724	3.950349528475436
4	0.716529791104007	2.013903681183556
5	0.3169266383729262	1.1134563621928313
6	0.1681089125282478	0.7087391801088283
7	0.11574712010141651	0.5693150791038128
8	0.11574712010141651	0.6506458046900718
9	0.05236179242683128	0.33113223988691154
>10	0.4133825717907733	5.770608624929804
>50	0.027558838119384886	1.3012916093801437
>100	0.02204707049550791	2.122344648631901
>500	0.005511767623876978	2.767181115780097
>1k	0.005511767623876978	5.4026839139443465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1498	2.900795879243237	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1292	2.5018880347011097	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	873	1.6905172246858118	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	1.0766638910942856	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	167	0.32338645649774406	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152	0.2943397687883658	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139	0.2691659727735714	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.26529308107898764	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.25561085184252824	No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA	131	0.25367440599523633	No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA	123	0.23818283921690128	No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.22269127243856626	No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT	88	0.1704072345616855	No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTGTCT	82	0.1587885594779342	No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA	76	0.1471698843941829	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	67	0.12974187176855598	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67	0.12974187176855598	No Hit
CAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.12005964253209658	No Hit
GCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.11618675083751283	No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACCTGTC	58	0.11231385914292906	No Hit
GTGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTC	57	0.11037741329563719	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.10650452160105342	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0019364458472918805	0.0
12	0.0	0.0	0.0	0.003872891694583761	0.0
13	0.0	0.0	0.0	0.003872891694583761	0.0
14	0.0	0.0	0.0	0.003872891694583761	0.0
15	0.0	0.0	0.0	0.003872891694583761	0.0
16	0.0	0.0	0.0	0.003872891694583761	0.0
17	0.0	0.0	0.0	0.005809337541875641	0.0
18	0.0	0.0	0.0	0.005809337541875641	0.0
19	0.0	0.0	0.0	0.005809337541875641	0.0
20	0.0	0.0	0.0	0.007745783389167522	0.0
21	0.0	0.0	0.0	0.009682229236459403	0.0
22	0.0	0.0	0.0	0.015491566778335044	0.0
23	0.0	0.0	0.0	0.025173796014794445	0.0
24	0.0	0.0	0.0	0.03679247109854573	0.0
25	0.0	0.0	0.0	0.04841114618229701	0.0
26	0.0	0.0	0.0	0.07164849634979958	0.0
27	0.0	0.0	0.0	0.18396235549272866	0.0
28	0.0	0.0	0.0	0.4124629654731705	0.0
29	0.0	0.0	0.0	0.745531651207374	0.0
30	0.0	0.0	0.0	1.103774132956372	0.0
31	0.0	0.0	0.0	1.6788985496020603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	475	0.0	21.031578	1
GCCTGTA	60	9.074896E-4	18.5	2
CCTGTAC	65	0.0015524632	17.076923	3
TGCCTGT	65	0.0015524632	17.076923	1
TGTACTG	80	3.3049413E-4	16.1875	5
CCTCCCC	75	0.004034206	14.8	12
CTGTACT	75	0.004034206	14.8	4
ACTGCCT	90	8.0868404E-4	14.388888	8
GTATCAA	700	0.0	14.271428	2
GTACTGC	80	0.0061917813	13.875	6
TTATACA	80	0.0061917813	13.875	37
TGCCTCC	80	0.0061917813	13.875	10
GGGGGAG	165	2.4362453E-7	13.454545	19
CTAAAAA	125	4.6716865E-5	13.32	10
GCCTCCC	85	0.00924652	13.058824	11
TACTGCC	85	0.00924652	13.058824	7
CTTATAC	115	3.4751816E-4	12.869564	37
TCTCTTA	265	2.7284841E-11	12.566038	37
AACGCAG	970	0.0	12.206185	5
ACGGGGG	155	2.6037114E-5	11.935483	17
>>END_MODULE