FastQCFastQC Report
Fri 10 Feb 2017
ERR1630044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673383
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81720.30568010644191274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74670.2793090253061383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57700.21583140163605438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41750.1561691684281676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.027.1951
TAGCACG358.872486E-426.428574
GTATCAA32750.016.3251912
CGTGCGA1708.54925E-1116.3235310
CTAATCG908.279076E-414.38888826
CGAATTA2201.8189894E-1114.29545415
TAGGACA8500.013.2764714
GACCGAC850.00940899613.0588247
TATACTG3800.012.6578955
CTTATAC23100.012.6536837
TCTTATA37400.012.41577537
GACCGTC1951.4695433E-712.3333347
TACGGGT1951.4695433E-712.3333344
CATATAC3750.012.3333333
GTATTGA3001.8189894E-1212.3333331
CGTCTAT1653.8082799E-612.3333331
TATCAAC44400.012.2083333
ATCAACG43800.012.206624
TCAACGC43850.012.1927035
TAGTCCG2756.184564E-1112.1090915
Produced by FastQC (version 0.11.5)