FastQCFastQC Report
Fri 10 Feb 2017
ERR1630043.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630043.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574779
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57950.3679881430981744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53840.34188924287153943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43040.27330819118111177No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC38840.24663778219038987No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.20174259372267472No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT25630.16275299581719085No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG20360.12928798263121366No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG17600.11176171386588214No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.1055386184347137No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT16330.10369709019487815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.030.1131381
CGTGCGA652.6838934E-622.7692310
CTGCGTA502.7027182E-422.1999999
ACTGCGT1001.2907549E-820.3499988
TATCCCG609.238884E-418.55
GACAGGC11950.018.4225947
GTATCAA22300.018.417042
GCATCAG12000.018.19166815
CCGAATT2550.017.41176414
TGCATCA12550.017.39442314
CGAATTA2450.017.36734815
GCCTATC650.001580285117.0769237
CGGTCGA1201.04189894E-716.95833420
TTGACGG1554.0199666E-1016.70967937
CGCAAGT1554.0199666E-1016.70967912
CCAGGAC16700.016.5059873
CAGGACA14800.016.54
GCTGCAT13200.016.39772812
CTGCATC13500.016.30740713
ACAGGCT14000.016.1214288
Produced by FastQC (version 0.11.5)