##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630043.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1574779
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.793725976787854	34.0	31.0	34.0	31.0	34.0
2	33.03281920828256	34.0	33.0	34.0	31.0	34.0
3	33.14880056185662	34.0	33.0	34.0	31.0	34.0
4	36.456375148512905	37.0	37.0	37.0	35.0	37.0
5	36.40497936535857	37.0	37.0	37.0	35.0	37.0
6	36.44679793164628	37.0	37.0	37.0	35.0	37.0
7	36.37729357579698	37.0	37.0	37.0	35.0	37.0
8	36.38022351072754	37.0	37.0	37.0	35.0	37.0
9	38.15556532059419	39.0	39.0	39.0	37.0	39.0
10	38.12949626582524	39.0	39.0	39.0	37.0	39.0
11	38.16336578021424	39.0	39.0	39.0	37.0	39.0
12	38.09840047397127	39.0	39.0	39.0	37.0	39.0
13	38.097587661506786	39.0	39.0	39.0	37.0	39.0
14	39.53857461904178	41.0	39.0	41.0	37.0	41.0
15	39.52448311794861	41.0	39.0	41.0	37.0	41.0
16	39.4428145155606	41.0	39.0	41.0	36.0	41.0
17	39.36172377203405	41.0	39.0	41.0	36.0	41.0
18	39.20257699651824	41.0	39.0	41.0	36.0	41.0
19	39.2398711184236	41.0	39.0	41.0	36.0	41.0
20	39.46721349471894	41.0	39.0	41.0	37.0	41.0
21	39.484548625553174	41.0	39.0	41.0	37.0	41.0
22	39.49389850893363	41.0	39.0	41.0	37.0	41.0
23	39.41747445197072	41.0	39.0	41.0	36.0	41.0
24	39.37119938734261	41.0	39.0	41.0	36.0	41.0
25	39.34034362916955	41.0	39.0	41.0	36.0	41.0
26	39.168717642285046	41.0	39.0	41.0	36.0	41.0
27	39.08698807896219	41.0	39.0	41.0	35.0	41.0
28	39.017611995079946	41.0	39.0	41.0	35.0	41.0
29	38.90275333872245	40.0	39.0	41.0	35.0	41.0
30	38.803320338917395	40.0	39.0	41.0	35.0	41.0
31	38.67896257189104	40.0	38.0	41.0	35.0	41.0
32	38.58418990855225	40.0	38.0	41.0	35.0	41.0
33	38.497498379137646	40.0	38.0	41.0	35.0	41.0
34	38.4125867820183	40.0	38.0	41.0	35.0	41.0
35	38.25158831810686	40.0	38.0	41.0	34.0	41.0
36	38.229271535879	40.0	38.0	41.0	34.0	41.0
37	38.168200109348675	40.0	38.0	41.0	34.0	41.0
38	38.059655989824606	40.0	38.0	41.0	34.0	41.0
39	38.00903301352126	40.0	38.0	41.0	33.0	41.0
40	37.92046757037019	40.0	38.0	41.0	33.0	41.0
41	37.80184838634501	40.0	38.0	41.0	33.0	41.0
42	37.710417779256645	40.0	38.0	41.0	33.0	41.0
43	37.40167921975083	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	5.0
14	6.0
15	11.0
16	17.0
17	45.0
18	63.0
19	155.0
20	293.0
21	533.0
22	904.0
23	1421.0
24	2100.0
25	3107.0
26	4395.0
27	6078.0
28	8039.0
29	10607.0
30	13640.0
31	17258.0
32	22050.0
33	28537.0
34	39663.0
35	55810.0
36	81479.0
37	142395.0
38	294149.0
39	842015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.495373001544976	18.48449845978388	11.953867812562907	26.066260726108236
2	19.209171572646067	20.48744617498709	35.11152993531156	25.19185231705528
3	19.337761044565617	22.61936436795258	30.66665227311261	27.37622231436919
4	14.4718084251822	15.52516257836814	35.19789125966247	34.805137736787195
5	14.706508024300552	36.32966911547589	34.204418524758076	14.759404335465485
6	34.521161382009794	35.27206039704619	15.218579876922414	14.988198344021605
7	29.018293995538418	30.294219061849315	21.712633963241824	18.97485297937044
8	27.012298233593413	33.859925741961256	19.78118834452326	19.346587679922074
9	26.492415761195698	14.25507960164569	19.850404405951565	39.402100231207044
10	17.07934891181556	26.68806226143478	33.3857004697167	22.846888357032956
11	34.86533665993768	21.865353805200602	22.053761194427917	21.215548340433802
12	21.526131603228134	25.10104592453925	30.134895118616644	23.237927353615966
13	29.721757783155606	19.834656164452284	25.2225867883684	25.220999264023714
14	21.736637331333476	21.120233378778863	26.535532922397365	30.6075963674903
15	24.778968985489392	27.6894726180626	23.051170989707124	24.480387406740885
16	23.637792985555432	26.955210858158512	24.403424226510513	25.003571929775543
17	23.155630091587454	26.815000708035857	26.530643347415733	23.498725852960956
18	23.142675892934818	25.3428576327218	27.07370367524586	24.440762799097524
19	24.15735795308421	25.29408888485305	27.275065263125807	23.27348789893693
20	23.943232669472987	25.842483294481323	26.912347700851992	23.3019363351937
21	24.790716665640065	25.2832302183354	26.454950186661115	23.471102929363422
22	24.353385459166017	25.828513080248083	26.65567676480319	23.16242469578271
23	24.169804143946546	25.740183225709767	26.24869902379953	23.841313606544155
24	24.166883099152326	25.474622153330724	26.53642193603039	23.822072811486564
25	24.020894360415017	25.13876550296899	27.07745023269932	23.762889903916676
26	24.281375354891065	25.67547573342037	26.940415131266036	23.102733780422525
27	24.076457712479023	26.0249850931464	26.209455421998896	23.68910177237568
28	23.580896113041895	25.505293123670054	27.106660680641536	23.807150082646515
29	23.5525746787327	25.519263337903286	27.461123116322987	23.467038867041026
30	23.38474160501251	25.640804201732433	27.09180145277528	23.882652740479777
31	23.340608428230247	25.64613828353058	27.047604775019224	23.96564851321995
32	23.082159464915396	25.407247620142254	27.627559168619854	23.8830337463225
33	23.668400454920977	25.61737234240487	27.25144290087689	23.462784301797267
34	24.067440574201203	25.300311980284217	26.896980465195437	23.735266980319143
35	23.772097545115855	25.249320698332912	27.496302655801223	23.48227910075001
36	23.998161011799116	25.126255811132864	26.627101326598847	24.24848185046918
37	22.891212036736583	24.22263695413769	28.16903197210529	24.71711903702043
38	23.332543804559243	24.32728655893938	28.623381439554375	23.716788196947
39	22.024423744538122	24.697751240015265	29.06680873951202	24.21101627593459
40	22.433052510860254	23.66408238870343	29.857014857322838	24.045850243113478
41	21.60366629222259	23.868365021377603	29.928453452833697	24.59951523356611
42	21.296321579091416	24.692099653348183	29.29414222567103	24.71743654188937
43	21.329977095198753	23.563052339407626	30.155405933149986	24.951564632243635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2591.0
1	1839.0
2	1087.0
3	1938.5
4	2790.0
5	2790.0
6	2963.0
7	3136.0
8	2615.0
9	2094.0
10	2917.5
11	3741.0
12	3741.0
13	6045.5
14	8350.0
15	10621.0
16	12892.0
17	13247.5
18	13603.0
19	13603.0
20	17343.0
21	21083.0
22	21141.5
23	21200.0
24	24909.0
25	28618.0
26	28618.0
27	30851.0
28	33084.0
29	37218.0
30	41352.0
31	46729.0
32	52106.0
33	52106.0
34	57544.5
35	62983.0
36	68680.0
37	74377.0
38	76349.5
39	78322.0
40	78322.0
41	82478.5
42	86635.0
43	89133.0
44	91631.0
45	91394.5
46	91158.0
47	91158.0
48	91097.0
49	91036.0
50	95939.5
51	100843.0
52	104585.5
53	108328.0
54	108328.0
55	106251.0
56	104174.0
57	97726.5
58	91279.0
59	83715.5
60	76152.0
61	76152.0
62	73005.5
63	69859.0
64	61286.5
65	52714.0
66	45317.5
67	37921.0
68	37921.0
69	32278.0
70	26635.0
71	23797.0
72	20959.0
73	22128.5
74	23298.0
75	23298.0
76	21855.5
77	20413.0
78	15757.0
79	11101.0
80	7492.0
81	3883.0
82	3883.0
83	2761.0
84	1639.0
85	1232.0
86	825.0
87	639.0
88	453.0
89	453.0
90	358.5
91	264.0
92	183.0
93	102.0
94	70.0
95	38.0
96	38.0
97	24.5
98	11.0
99	15.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1574779.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.723976278516155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.4667677451368	47.912909298980736
2	9.67578446207667	10.976979366057444
3	2.467962721684401	4.199779766432644
4	0.9755896414230103	2.2135729471057966
5	0.5367276645467446	1.5222663655886457
6	0.3312610674969434	1.1274266960815533
7	0.21862088058812404	0.8680731951128342
8	0.15570061333449284	0.706556631786895
9	0.11854730226471895	0.6052026925391402
>10	0.8488051814316375	9.662278688156798
>50	0.1128985889962507	4.485330948731157
>100	0.07994568732227186	8.931081861027485
>500	0.008118494517354344	3.1511261498758247
>1k	0.0030444354440078785	2.9224045489673887
>5k	2.2551373659317623E-4	0.7150108435557645
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5795	0.3679881430981744	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5384	0.34188924287153943	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4304	0.27330819118111177	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3884	0.24663778219038987	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3177	0.20174259372267472	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2563	0.16275299581719085	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2036	0.12928798263121366	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1760	0.11176171386588214	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1662	0.1055386184347137	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1633	0.10369709019487815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.350097378743303E-5	0.0	0.0	0.0	0.0
8	6.350097378743303E-5	0.0	0.0	0.0	0.0
9	6.350097378743303E-5	0.0	0.0	2.540038951497321E-4	0.0
10	6.350097378743303E-5	0.0	0.0	3.1750486893716514E-4	0.0
11	6.350097378743303E-5	0.0	0.0	3.1750486893716514E-4	0.0
12	6.350097378743303E-5	0.0	0.0	4.445068165120312E-4	0.0
13	6.350097378743303E-5	0.0	0.0	5.080077902994642E-4	0.0
14	6.350097378743303E-5	0.0	0.0	5.715087640868972E-4	0.0
15	6.350097378743303E-5	0.0	0.0	5.715087640868972E-4	0.0
16	1.2700194757486605E-4	0.0	0.0	5.715087640868972E-4	0.0
17	1.905029213622991E-4	0.0	0.0	6.350097378743303E-4	0.0
18	1.905029213622991E-4	0.0	0.0	8.255126592366294E-4	0.0
19	1.905029213622991E-4	0.0	0.0	0.0010160155805989284	0.0
20	1.905029213622991E-4	0.0	0.0	0.0013335204495360936	0.0
21	1.905029213622991E-4	0.0	0.0	0.0016510253184732588	0.0
22	1.905029213622991E-4	0.0	0.0	0.0027305418728596205	0.0
23	1.905029213622991E-4	0.0	0.0	0.004318066217545446	0.0
24	1.905029213622991E-4	0.0	0.0	0.009525146068114955	0.0
25	1.905029213622991E-4	0.0	0.0	0.016700756106094887	0.0
26	1.905029213622991E-4	0.0	0.0	0.03321100929082747	0.0
27	2.540038951497321E-4	0.0	0.0	0.1040145950638153	0.0
28	2.540038951497321E-4	0.0	0.0	0.23063553679595677	0.0
29	2.540038951497321E-4	0.0	0.0	0.38862595957909013	0.0
30	3.810058427245982E-4	0.0	0.0	0.5825579335259106	0.0
31	3.810058427245982E-4	0.0	0.0	0.9274952231392468	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1370	0.0	30.113138	1
CGTGCGA	65	2.6838934E-6	22.76923	10
CTGCGTA	50	2.7027182E-4	22.199999	9
ACTGCGT	100	1.2907549E-8	20.349998	8
TATCCCG	60	9.238884E-4	18.5	5
GACAGGC	1195	0.0	18.422594	7
GTATCAA	2230	0.0	18.41704	2
GCATCAG	1200	0.0	18.191668	15
CCGAATT	255	0.0	17.411764	14
TGCATCA	1255	0.0	17.394423	14
CGAATTA	245	0.0	17.367348	15
GCCTATC	65	0.0015802851	17.076923	7
CGGTCGA	120	1.04189894E-7	16.958334	20
TTGACGG	155	4.0199666E-10	16.709679	37
CGCAAGT	155	4.0199666E-10	16.709679	12
CCAGGAC	1670	0.0	16.505987	3
CAGGACA	1480	0.0	16.5	4
GCTGCAT	1320	0.0	16.397728	12
CTGCATC	1350	0.0	16.307407	13
ACAGGCT	1400	0.0	16.121428	8
>>END_MODULE