FastQCFastQC Report
Fri 10 Feb 2017
ERR1630042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3164537
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104540.33034848383823606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96460.3048155227763177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78460.24793516397501433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54280.17152588198526356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.027.189981
GTATCAA37850.017.0581252
CGAATTA3700.015.015
GTATTAG5100.014.5098041
TATACTG5000.013.695
ACGTTAC950.00124591813.6315791
CCGAATT4300.012.90697714
CTATACT6200.012.8306454
TACACCG1602.6990474E-612.7187515
TACGTTA2204.092726E-912.61363619
ATTACGT2358.112693E-1012.59574517
GTATAGA4000.012.4874991
GTATGCG1050.002645334712.3333343
CCCGATT1050.002645334712.33333413
GTCTAGA3500.012.1571441
TAGAGAG8250.012.109095
TGCGCAA2603.092282E-1012.09615410
CTTATAC27850.012.08976737
ATTATAC7000.011.8928583
GGACCGT1401.399792E-411.8928576
Produced by FastQC (version 0.11.5)