##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630042.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3164537
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8435132848818	34.0	31.0	34.0	31.0	34.0
2	33.06475512847535	34.0	33.0	34.0	31.0	34.0
3	33.1950174701702	34.0	33.0	34.0	31.0	34.0
4	36.474742118673284	37.0	37.0	37.0	35.0	37.0
5	36.43050341961557	37.0	37.0	37.0	35.0	37.0
6	36.47751598417083	37.0	37.0	37.0	35.0	37.0
7	36.40728738516883	37.0	37.0	37.0	35.0	37.0
8	36.41386180664028	37.0	37.0	37.0	35.0	37.0
9	38.19320109071248	39.0	39.0	39.0	37.0	39.0
10	38.171887388265645	39.0	39.0	39.0	37.0	39.0
11	38.206024767604234	39.0	39.0	39.0	37.0	39.0
12	38.136349172090576	39.0	39.0	39.0	37.0	39.0
13	38.155114950465105	39.0	39.0	39.0	37.0	39.0
14	39.60799478723112	41.0	40.0	41.0	37.0	41.0
15	39.59652612688681	41.0	40.0	41.0	37.0	41.0
16	39.52257028437335	41.0	39.0	41.0	37.0	41.0
17	39.452924709049064	41.0	39.0	41.0	37.0	41.0
18	39.35558566703439	41.0	39.0	41.0	36.0	41.0
19	39.378805493505055	41.0	39.0	41.0	36.0	41.0
20	39.6019787412819	41.0	40.0	41.0	37.0	41.0
21	39.61514464833244	41.0	40.0	41.0	37.0	41.0
22	39.625014970594435	41.0	40.0	41.0	37.0	41.0
23	39.55892283768526	41.0	40.0	41.0	37.0	41.0
24	39.53192457538022	41.0	40.0	41.0	37.0	41.0
25	39.50631229781798	41.0	40.0	41.0	37.0	41.0
26	39.37496606928597	41.0	39.0	41.0	36.0	41.0
27	39.29252367723936	41.0	39.0	41.0	36.0	41.0
28	39.25442394890627	41.0	39.0	41.0	36.0	41.0
29	39.14972522046669	41.0	39.0	41.0	36.0	41.0
30	39.06956720682994	41.0	39.0	41.0	35.0	41.0
31	38.94613335220919	40.0	39.0	41.0	35.0	41.0
32	38.860988195113535	40.0	39.0	41.0	35.0	41.0
33	38.78709112897084	40.0	39.0	41.0	35.0	41.0
34	38.706343455614515	40.0	38.0	41.0	35.0	41.0
35	38.5875153300467	40.0	38.0	41.0	35.0	41.0
36	38.55935923643806	40.0	38.0	41.0	35.0	41.0
37	38.51604895123678	40.0	38.0	41.0	35.0	41.0
38	38.419398161563606	40.0	38.0	41.0	35.0	41.0
39	38.377864123566894	40.0	38.0	41.0	35.0	41.0
40	38.31701951975913	40.0	38.0	41.0	34.0	41.0
41	38.2114312457083	40.0	38.0	41.0	34.0	41.0
42	38.11667962801509	40.0	38.0	41.0	34.0	41.0
43	37.8240342900083	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	5.0
14	7.0
15	16.0
16	23.0
17	36.0
18	62.0
19	139.0
20	346.0
21	590.0
22	1045.0
23	1861.0
24	3092.0
25	4526.0
26	6702.0
27	9688.0
28	13513.0
29	17755.0
30	23249.0
31	30574.0
32	39245.0
33	51341.0
34	69954.0
35	98625.0
36	150425.0
37	267923.0
38	570468.0
39	1803325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.879448083558515	19.751072589765897	12.121488862351743	25.24799046432385
2	18.473571331287957	21.427083962045632	35.695711568548575	24.403633138117836
3	18.849676903761907	23.478000099224623	31.293456199121707	26.37886679789176
4	13.841424511705819	15.917715608950061	36.62624263833856	33.614617241005554
5	13.914547372964828	36.80588977155268	35.37275753135451	13.906805324127985
6	33.38188177291022	36.81252581341283	15.460808326778924	14.344784086898022
7	27.810229426927226	31.322812784303046	22.41244137768021	18.45451641108952
8	25.889348110007877	34.947166046723424	20.390818625283888	18.77266721798481
9	25.67193873858956	14.493336623967423	21.019788992828968	38.814935644614046
10	15.651231127965955	27.495491441560016	35.30712391733767	21.54615351313636
11	34.39615337093546	22.284176168583272	22.860911406629153	20.458759053852113
12	20.145569478252266	26.01119847864	31.595490904356627	22.247741138751103
13	29.556772444120575	21.005916505321316	25.43951927248757	23.99779177807054
14	21.177157985512572	21.965424957900634	26.975668162514772	29.881748894072025
15	23.695820273234283	28.997764917901105	23.73908094612261	23.567333862742007
16	23.126890284423915	27.919534516423727	25.351070314551542	23.602504884600812
17	22.06594519198227	27.469042074717407	27.464586446611307	23.000426286689017
18	22.47870699568373	26.592136543197313	27.990508564128024	22.938647896990936
19	23.187120264354625	26.268013298627892	28.329389101786457	22.215477335231032
20	23.147841216582393	26.54947627409634	28.018000737548654	22.284681771772615
21	22.932106655728784	26.586985710705864	27.66433130660188	22.81657632696347
22	22.829153206298425	26.84086171215568	27.745354217694405	22.58463086385149
23	23.001658694463046	26.590840935024616	27.619174621753512	22.788325748758822
24	23.04649937731807	26.562495556221972	27.493311027805962	22.897694038653995
25	23.143891191665634	26.522837306057728	27.57139512036042	22.761876381916217
26	22.826783191348373	26.649301303792623	27.634848320623206	22.8890671842358
27	22.47216575442158	26.88567079481137	27.841829626261283	22.800333824505763
28	22.664263366173316	26.782875346377683	27.670272144076684	22.882589143372318
29	22.733657403910904	26.781390137008987	27.982260912101836	22.50269154697828
30	22.790442962114206	26.890505625309487	27.545293355710488	22.773758056865823
31	22.8848011573257	26.80063465840343	27.61683620700279	22.69772797726808
32	22.386718815422288	26.804079080130837	27.94443547349897	22.86476663094791
33	22.376985954027397	26.6191231134286	28.105217287710648	22.89867364483335
34	22.687995115873193	26.1739395052104	28.05156014924142	23.086505229674987
35	22.37186672173528	26.298918293576595	28.346864012018187	22.982350972669934
36	22.5167220354826	26.126412805411974	28.26283908198893	23.094026077116496
37	22.358341836420305	25.508628908431152	28.66738483386353	23.46564442128501
38	21.96296014235258	25.62491764198049	29.26029937396845	23.151822841698486
39	21.634128468082377	25.66030986523463	29.607237962457067	23.098323704225926
40	21.675366728213323	25.190825703728535	29.909146266894655	23.224661301163486
41	21.235333952486574	25.042462767855138	30.24553038880569	23.476672890852594
42	20.81546210393495	25.451685349231184	29.946339701510837	23.78651284532303
43	20.71007543915587	25.05115282267201	30.510024057231753	23.72874768094037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3680.0
1	2762.5
2	1845.0
3	3446.5
4	5048.0
5	5048.0
6	5474.5
7	5901.0
8	5225.5
9	4550.0
10	5966.5
11	7383.0
12	7383.0
13	11763.0
14	16143.0
15	20687.5
16	25232.0
17	26882.5
18	28533.0
19	28533.0
20	36583.5
21	44634.0
22	45846.0
23	47058.0
24	55923.5
25	64789.0
26	64789.0
27	75177.0
28	85565.0
29	93926.0
30	102287.0
31	112122.0
32	121957.0
33	121957.0
34	134227.5
35	146498.0
36	155927.5
37	165357.0
38	173195.0
39	181033.0
40	181033.0
41	185799.0
42	190565.0
43	195675.0
44	200785.0
45	200034.5
46	199284.0
47	199284.0
48	201245.5
49	203207.0
50	201797.5
51	200388.0
52	199301.0
53	198214.0
54	198214.0
55	192787.5
56	187361.0
57	177621.0
58	167881.0
59	158901.0
60	149921.0
61	149921.0
62	136582.5
63	123244.0
64	108318.0
65	93392.0
66	80354.5
67	67317.0
68	67317.0
69	57741.5
70	48166.0
71	40033.0
72	31900.0
73	26056.0
74	20212.0
75	20212.0
76	15941.0
77	11670.0
78	8998.5
79	6327.0
80	4971.5
81	3616.0
82	3616.0
83	2742.5
84	1869.0
85	1400.0
86	931.0
87	669.0
88	407.0
89	407.0
90	318.5
91	230.0
92	156.0
93	82.0
94	62.0
95	42.0
96	42.0
97	30.0
98	18.0
99	16.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3164537.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.067228903057114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70963907391074	49.2871400400963
2	11.284400484428915	13.782141348327784
3	3.429300481012295	6.282536323540251
4	1.5047918326616185	3.6757386918639146
5	0.822545260767843	2.5115279861217332
6	0.4980055597816553	1.8247091708508862
7	0.3453850207760674	1.476419428639347
8	0.2298843988951583	1.1230722566857858
9	0.16910608502642968	0.9294156002887938
>10	0.9050504205600014	9.88486006498488
>50	0.061749290005817616	2.5776334960707294
>100	0.03760131598047013	4.238207516097351
>500	0.0017111349876300726	0.7622382164316899
>1k	6.222309039518659E-4	0.58757445719565
>5k	1.5555772598796648E-4	0.7257602155057287
>10k+	5.1852575329322165E-5	0.3310251872991661
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10454	0.33034848383823606	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9646	0.3048155227763177	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7846	0.24793516397501433	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5428	0.17152588198526356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.32003986681148E-5	0.0
5	0.0	0.0	0.0	6.32003986681148E-5	0.0
6	0.0	3.16001993340574E-5	0.0	6.32003986681148E-5	0.0
7	0.0	3.16001993340574E-5	0.0	6.32003986681148E-5	0.0
8	0.0	3.16001993340574E-5	0.0	6.32003986681148E-5	0.0
9	0.0	3.16001993340574E-5	0.0	9.48005980021722E-5	0.0
10	0.0	3.16001993340574E-5	0.0	2.212013953384018E-4	0.0
11	0.0	3.16001993340574E-5	0.0	2.212013953384018E-4	0.0
12	0.0	3.16001993340574E-5	0.0	3.792023920086888E-4	0.0
13	0.0	3.16001993340574E-5	0.0	3.792023920086888E-4	0.0
14	0.0	6.32003986681148E-5	0.0	3.792023920086888E-4	0.0
15	0.0	6.32003986681148E-5	0.0	4.74002990010861E-4	0.0
16	0.0	6.32003986681148E-5	0.0	5.688035880130332E-4	0.0
17	0.0	6.32003986681148E-5	0.0	5.688035880130332E-4	0.0
18	0.0	6.32003986681148E-5	0.0	5.688035880130332E-4	0.0
19	0.0	6.32003986681148E-5	0.0	6.320039866811479E-4	0.0
20	0.0	6.32003986681148E-5	0.0	8.848055813536072E-4	0.0
21	0.0	6.32003986681148E-5	0.0	0.001358808571364468	0.0
22	0.0	6.32003986681148E-5	0.0	0.001959212358711559	0.0
23	0.0	6.32003986681148E-5	0.0	0.00316001993340574	0.0
24	0.0	6.32003986681148E-5	0.0	0.0069836440528266855	0.0
25	0.0	6.32003986681148E-5	0.0	0.011565672956265009	0.0
26	0.0	6.32003986681148E-5	0.0	0.020919331959145998	0.0
27	0.0	6.32003986681148E-5	0.0	0.0760300795977421	0.0
28	0.0	6.32003986681148E-5	0.0	0.1723790873672831	0.0
29	0.0	9.48005980021722E-5	0.0	0.2815893762657855	0.0
30	0.0	9.48005980021722E-5	0.0	0.4088433789840346	0.0
31	0.0	9.48005980021722E-5	0.0	0.6674594103339604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2395	0.0	27.18998	1
GTATCAA	3785	0.0	17.058125	2
CGAATTA	370	0.0	15.0	15
GTATTAG	510	0.0	14.509804	1
TATACTG	500	0.0	13.69	5
ACGTTAC	95	0.001245918	13.631579	1
CCGAATT	430	0.0	12.906977	14
CTATACT	620	0.0	12.830645	4
TACACCG	160	2.6990474E-6	12.718751	5
TACGTTA	220	4.092726E-9	12.613636	19
ATTACGT	235	8.112693E-10	12.595745	17
GTATAGA	400	0.0	12.487499	1
GTATGCG	105	0.0026453347	12.333334	3
CCCGATT	105	0.0026453347	12.333334	13
GTCTAGA	350	0.0	12.157144	1
TAGAGAG	825	0.0	12.10909	5
TGCGCAA	260	3.092282E-10	12.096154	10
CTTATAC	2785	0.0	12.089767	37
ATTATAC	700	0.0	11.892858	3
GGACCGT	140	1.399792E-4	11.892857	6
>>END_MODULE