FastQCFastQC Report
Fri 10 Feb 2017
ERR1630041.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630041.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041125
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58510.2866556433339457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52210.25579031171535305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43510.21316675852777267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30510.14947639169575602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.026.7148021
CGTGCGA1103.6379788E-1223.54545410
CGTCTAG450.003826810720.5555551
ATCTCGC4000.016.187511
GCTATAC1151.2432738E-616.0869563
GTATCAA23050.016.052062
GTCGTAC700.002593417215.8571431
CGACCAA1751.3278623E-1015.85714212
AATCTCG4100.015.34146410
ACCGTCC855.366684E-415.2352948
GTTCTAT1954.1836756E-1115.1794871
CGACGTT750.004105856614.824
TAGCACT4150.014.7108434
ACGTTAA1904.5656634E-1014.60526420
TGCGAGA1651.6470949E-814.57575712
TATACGA1551.2114651E-714.32258136
TCGTTGT1956.730261E-1014.2307734
CGAATTA2405.456968E-1213.87499915
GTATTAT3350.013.805971
CGGCCTT4300.013.76744224
Produced by FastQC (version 0.11.5)