##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630041.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2041125
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.553613815910346	33.0	31.0	34.0	31.0	34.0
2	32.82277322554964	34.0	31.0	34.0	31.0	34.0
3	32.96864866188989	34.0	31.0	34.0	31.0	34.0
4	36.364493600342946	37.0	37.0	37.0	35.0	37.0
5	36.27888247902505	37.0	37.0	37.0	35.0	37.0
6	36.34063518892768	37.0	37.0	37.0	35.0	37.0
7	36.27369808316492	37.0	37.0	37.0	35.0	37.0
8	36.27694776165105	37.0	37.0	37.0	35.0	37.0
9	37.993500643027744	39.0	38.0	39.0	35.0	39.0
10	37.9288343438055	39.0	38.0	39.0	35.0	39.0
11	37.99075264866189	39.0	38.0	39.0	35.0	39.0
12	37.90200306203687	39.0	38.0	39.0	35.0	39.0
13	37.93004299099761	39.0	38.0	39.0	35.0	39.0
14	39.30731679833425	40.0	39.0	41.0	36.0	41.0
15	39.291358442035644	40.0	39.0	41.0	36.0	41.0
16	39.205935942188745	40.0	39.0	41.0	36.0	41.0
17	39.12734692877702	40.0	39.0	41.0	36.0	41.0
18	39.00290281094984	40.0	38.0	41.0	36.0	41.0
19	39.03356923265356	40.0	39.0	41.0	36.0	41.0
20	39.246579704819645	40.0	39.0	41.0	36.0	41.0
21	39.24535293036928	40.0	39.0	41.0	36.0	41.0
22	39.26852005634148	40.0	39.0	41.0	36.0	41.0
23	39.200214587543634	40.0	39.0	41.0	36.0	41.0
24	39.15409590299467	40.0	39.0	41.0	36.0	41.0
25	39.13003466225733	40.0	39.0	41.0	36.0	41.0
26	38.96790054504256	40.0	39.0	41.0	35.0	41.0
27	38.86817514850879	40.0	39.0	41.0	35.0	41.0
28	38.82144748606773	40.0	38.0	41.0	35.0	41.0
29	38.70588817441362	40.0	38.0	41.0	35.0	41.0
30	38.60994745544736	40.0	38.0	41.0	35.0	41.0
31	38.47925090330087	40.0	38.0	41.0	35.0	41.0
32	38.392623185743155	40.0	38.0	41.0	34.0	41.0
33	38.31097850450119	40.0	38.0	41.0	34.0	41.0
34	38.23921709841387	40.0	38.0	41.0	34.0	41.0
35	38.09787984567334	40.0	38.0	41.0	34.0	41.0
36	38.08543964725335	40.0	38.0	41.0	34.0	41.0
37	38.04131986037112	40.0	38.0	41.0	34.0	41.0
38	37.93384628574928	40.0	38.0	41.0	33.0	41.0
39	37.88563120827975	40.0	38.0	41.0	33.0	41.0
40	37.81738698021924	40.0	38.0	41.0	33.0	41.0
41	37.7108638618409	40.0	38.0	41.0	33.0	41.0
42	37.612696429665014	40.0	37.0	41.0	33.0	41.0
43	37.299623002020944	40.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	13.0
17	31.0
18	86.0
19	142.0
20	264.0
21	539.0
22	956.0
23	1625.0
24	2604.0
25	3939.0
26	5540.0
27	7808.0
28	10787.0
29	14386.0
30	18555.0
31	23911.0
32	30923.0
33	40158.0
34	55541.0
35	78091.0
36	119512.0
37	215728.0
38	516385.0
39	893596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00344172943842	19.56264315022353	12.199595811133566	25.234319309204484
2	18.615175454712475	21.7071467940474	35.316210423173494	24.36146732806663
3	19.07159042194868	23.744552636413744	31.3475656806908	25.83629126094678
4	14.323865515340806	16.05467573029579	36.21576336579093	33.405695388572475
5	13.7591279319003	37.15010104721661	35.43036315757242	13.660407863310676
6	33.23074285014392	36.621471002510866	15.545642721538366	14.602143425806846
7	27.7075632310613	31.166342090758775	22.647265601077837	18.478829077102088
8	25.94201726988793	34.73422744809847	20.546512340008576	18.77724294200502
9	25.53753444791475	14.711788841937656	20.637344601629003	39.11333210851859
10	16.06021189295119	27.408120521771085	34.97571192357156	21.555955661706168
11	34.124661644926206	22.62507195786637	22.714679404740032	20.53558699246739
12	19.961393839181824	26.16988180537694	31.056402719088737	22.8123216363525
13	29.25161369342887	20.92654785963623	25.76779962030743	24.054038826627473
14	21.43136750566477	21.83976973482761	26.569416375773162	30.159446383734462
15	23.88163390287219	29.041729438422436	23.48239328801519	23.594243370690183
16	23.483422132402474	27.89040357645906	25.112670708555328	23.513503582583134
17	22.601016596239816	27.620013472962214	26.777389919774635	23.001580011023332
18	22.38628207483618	26.769551105395312	27.629077102088313	23.2150897176802
19	23.473868577377672	26.434637761038644	28.181419560291506	21.910074101292178
20	23.50012860554841	26.313038152979363	27.890795517178024	22.2960377242942
21	22.652654785963623	26.89369832812787	27.292400024496295	23.16124686141221
22	22.967922101782108	26.63006920203319	27.36422316124686	23.03778553493784
23	22.975907893931044	26.723057137607935	27.334141711066202	22.96689325739482
24	23.25256904893135	26.75965460224141	27.244093330883707	22.743683017943535
25	23.51218078265662	26.1067303570335	27.52046053034479	22.860628329965095
26	23.041166023638926	26.704880886765874	27.414538551044153	22.839414538551043
27	22.90413374977035	26.914667156592564	27.024312572723375	23.156886520913712
28	22.839561516320657	26.705909731153167	27.563623002020943	22.890905750505237
29	22.86092228550432	26.960720191071104	27.608941147651418	22.569416375773162
30	22.71805989344112	27.309106497642233	27.229052605793374	22.743781003123278
31	23.10549329413926	26.681315451038028	27.507967419927738	22.705223834894973
32	22.53350480739788	26.881058239941208	27.651466715659257	22.933970237001652
33	22.61924183967175	26.726094678179923	27.714716149182433	22.939947332965886
34	22.951068650866556	26.21554289913651	27.604041888664337	23.229346561332598
35	22.82550064302774	26.41959703594831	27.828868883581357	22.926033437442587
36	22.815506154694102	26.35125237307857	27.702321023945125	23.130920448282197
37	22.867438299957133	25.26778124808623	28.215028476942862	23.649751975013782
38	22.4412517606712	25.840553616265538	28.738906240431135	22.979288382632127
39	22.098303631575725	25.86955722946904	28.923020393165533	23.1091187457897
40	21.976802008696183	25.213938391818235	29.47355012554351	23.335709473942064
41	21.634833731398125	25.425929328189113	29.41015371425072	23.529083226162044
42	21.544540388266277	25.22667646518464	29.270769796068347	23.95801335048074
43	21.28860309878131	25.162153224324825	29.63586257578541	23.91338110110846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1470.0
1	1213.0
2	956.0
3	1745.0
4	2534.0
5	2534.0
6	2879.0
7	3224.0
8	2797.0
9	2370.0
10	3338.5
11	4307.0
12	4307.0
13	6646.5
14	8986.0
15	12121.5
16	15257.0
17	16225.0
18	17193.0
19	17193.0
20	21137.5
21	25082.0
22	27056.0
23	29030.0
24	33645.0
25	38260.0
26	38260.0
27	42574.5
28	46889.0
29	55831.5
30	64774.0
31	70939.0
32	77104.0
33	77104.0
34	83667.5
35	90231.0
36	98939.0
37	107647.0
38	112365.0
39	117083.0
40	117083.0
41	125434.0
42	133785.0
43	131339.0
44	128893.0
45	131591.5
46	134290.0
47	134290.0
48	135093.5
49	135897.0
50	139604.0
51	143311.0
52	144187.0
53	145063.0
54	145063.0
55	133140.0
56	121217.0
57	112823.5
58	104430.0
59	95736.5
60	87043.0
61	87043.0
62	80099.5
63	73156.0
64	65094.5
65	57033.0
66	49909.5
67	42786.0
68	42786.0
69	36423.5
70	30061.0
71	25445.0
72	20829.0
73	17186.0
74	13543.0
75	13543.0
76	10650.0
77	7757.0
78	6311.0
79	4865.0
80	3619.5
81	2374.0
82	2374.0
83	1828.0
84	1282.0
85	953.5
86	625.0
87	451.5
88	278.0
89	278.0
90	206.0
91	134.0
92	89.5
93	45.0
94	32.5
95	20.0
96	20.0
97	12.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2041125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.40106481427729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.43347395483833	48.726037201168296
2	9.773332062397412	11.41545998455051
3	2.7298562957045434	4.782795433773119
4	1.1763920058355515	2.748101831191988
5	0.6749261323106884	1.9708202398963002
6	0.43438107933752923	1.5220990541092063
7	0.2759541210719263	1.128121015734194
8	0.20704646428465087	0.9673387184203877
9	0.15815025448619166	0.8312528936378273
>10	0.9581363617994088	10.486610594235147
>50	0.10044743434749057	4.0506693746358895
>100	0.07232248997578436	8.0483088692908
>500	0.004397422238527573	1.8058478984245514
>1k	0.0010147897473525163	0.9697203195833735
>5k	1.6913162455875271E-4	0.5468165713484651
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5851	0.2866556433339457	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5221	0.25579031171535305	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4351	0.21316675852777267	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3051	0.14947639169575602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.899258987078204E-5	0.0
9	0.0	0.0	0.0	4.899258987078204E-5	0.0
10	0.0	0.0	0.0	4.899258987078204E-5	0.0
11	0.0	0.0	0.0	9.798517974156409E-5	0.0
12	0.0	0.0	0.0	1.9597035948312817E-4	0.0
13	0.0	0.0	0.0	1.9597035948312817E-4	0.0
14	0.0	0.0	0.0	2.449629493539102E-4	0.0
15	0.0	0.0	0.0	2.939555392246923E-4	0.0
16	0.0	0.0	0.0	3.429481290954743E-4	0.0
17	0.0	0.0	0.0	3.429481290954743E-4	0.0
18	0.0	0.0	0.0	3.429481290954743E-4	0.0
19	0.0	0.0	0.0	4.409333088370384E-4	0.0
20	0.0	0.0	0.0	5.879110784493846E-4	0.0
21	0.0	0.0	0.0	7.838814379325127E-4	0.0
22	0.0	0.0	0.0	0.001077836977157205	0.0
23	0.0	0.0	0.0	0.00215567395431441	0.0
24	0.0	0.0	0.0	0.0038214220099209995	0.0
25	0.0	0.0	0.0	0.007054932941392615	0.0
26	0.0	0.0	0.0	0.013717925163818973	0.0
27	0.0	0.0	0.0	0.05692938942984874	0.0
28	0.0	0.0	0.0	0.13409271847633045	0.0
29	0.0	0.0	0.0	0.22257333578296282	0.0
30	0.0	0.0	0.0	0.32188131545103804	0.0
31	0.0	0.0	0.0	0.5230448894604691	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1385	0.0	26.714802	1
CGTGCGA	110	3.6379788E-12	23.545454	10
CGTCTAG	45	0.0038268107	20.555555	1
ATCTCGC	400	0.0	16.1875	11
GCTATAC	115	1.2432738E-6	16.086956	3
GTATCAA	2305	0.0	16.05206	2
GTCGTAC	70	0.0025934172	15.857143	1
CGACCAA	175	1.3278623E-10	15.857142	12
AATCTCG	410	0.0	15.341464	10
ACCGTCC	85	5.366684E-4	15.235294	8
GTTCTAT	195	4.1836756E-11	15.179487	1
CGACGTT	75	0.0041058566	14.8	24
TAGCACT	415	0.0	14.710843	4
ACGTTAA	190	4.5656634E-10	14.605264	20
TGCGAGA	165	1.6470949E-8	14.575757	12
TATACGA	155	1.2114651E-7	14.322581	36
TCGTTGT	195	6.730261E-10	14.23077	34
CGAATTA	240	5.456968E-12	13.874999	15
GTATTAT	335	0.0	13.80597	1
CGGCCTT	430	0.0	13.767442	24
>>END_MODULE