FastQCFastQC Report
Fri 10 Feb 2017
ERR1630040.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630040.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304833
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20810.6826688711524015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.6531445086325955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13460.44155324390731976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.2863863164421175No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATT5220.17124130261487439No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAA4530.1486059580163565No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACT4140.13581206759110726No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAA4080.13384377675645354No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCG3900.12793890425249232No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC3770.1236742741107426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.12203403174853115No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.11317672299258938No Hit
GGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATC3430.1125206260477048No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACAT3320.10891209285083964No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAG3280.10759989896107049No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTAT3250.10661575354374363No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACC3060.10038283256734015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAG200.001839815237.01
CTCTAAT401.5949645E-632.3751
GCTCTAG303.59333E-430.8333321
AACTTCC250.005490086529.622
AGGGTTG250.005490086529.65
ATTTGGG250.005490086529.611
GCCGAGT453.995565E-628.77777712
CCGAGTT453.995565E-628.77777713
CCCCTTG405.9260365E-527.759
AGTCCAA405.9260365E-527.7530
ACTGTTA405.9260365E-527.7524
GAGTTCC405.9260365E-527.7515
GGTATCA4600.027.3478261
ATCTCGT358.854387E-426.42857237
GAATCTC358.854387E-426.42857235
CGAGTTC451.3200984E-424.66666614
ACCAGTA451.3200984E-424.66666612
CCCTTGT451.3200984E-424.66666610
CAGTATT451.3200984E-424.66666614
TAGTCCA451.3200984E-424.66666629
Produced by FastQC (version 0.11.5)