##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630040.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	304833
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.639815899197266	33.0	31.0	34.0	31.0	34.0
2	32.88122348958282	34.0	31.0	34.0	31.0	34.0
3	33.00625588436947	34.0	31.0	34.0	31.0	34.0
4	36.333697467137746	37.0	37.0	37.0	35.0	37.0
5	36.29898009729918	37.0	37.0	37.0	35.0	37.0
6	36.3912470106583	37.0	37.0	37.0	35.0	37.0
7	36.31646508088035	37.0	37.0	37.0	35.0	37.0
8	36.3444902618811	37.0	37.0	37.0	35.0	37.0
9	38.10262340363412	39.0	39.0	39.0	37.0	39.0
10	38.06374309868026	39.0	38.0	39.0	37.0	39.0
11	38.10277758641617	39.0	39.0	39.0	37.0	39.0
12	38.02375398988955	39.0	38.0	39.0	35.0	39.0
13	38.07420784495117	39.0	38.0	39.0	35.0	39.0
14	39.43476592101249	41.0	39.0	41.0	37.0	41.0
15	39.41736623003415	41.0	39.0	41.0	37.0	41.0
16	39.32222889254116	41.0	39.0	41.0	36.0	41.0
17	39.284076855196126	40.0	39.0	41.0	36.0	41.0
18	39.21916918443869	40.0	39.0	41.0	36.0	41.0
19	39.240318469457044	40.0	39.0	41.0	36.0	41.0
20	39.429333438308845	41.0	39.0	41.0	37.0	41.0
21	39.43087526612932	41.0	39.0	41.0	37.0	41.0
22	39.45112241784846	41.0	39.0	41.0	37.0	41.0
23	39.37452638001791	41.0	39.0	41.0	36.0	41.0
24	39.31881062745831	41.0	39.0	41.0	36.0	41.0
25	39.31960450476162	41.0	39.0	41.0	36.0	41.0
26	39.139240174128126	41.0	39.0	41.0	36.0	41.0
27	39.03289342033179	40.0	39.0	41.0	35.0	41.0
28	38.98736685332625	40.0	39.0	41.0	35.0	41.0
29	38.893715575413424	40.0	39.0	41.0	35.0	41.0
30	38.76833872973071	40.0	38.0	41.0	35.0	41.0
31	38.620264210239704	40.0	38.0	41.0	35.0	41.0
32	38.51169656828493	40.0	38.0	41.0	35.0	41.0
33	38.390046353249154	40.0	38.0	41.0	35.0	41.0
34	38.34808895362379	40.0	38.0	41.0	35.0	41.0
35	38.17031620592259	40.0	38.0	41.0	34.0	41.0
36	38.14343919457539	40.0	38.0	41.0	34.0	41.0
37	38.074965636922514	40.0	38.0	41.0	34.0	41.0
38	37.96158880436173	40.0	38.0	41.0	34.0	41.0
39	37.9036784075215	40.0	38.0	41.0	33.0	41.0
40	37.79844045756201	40.0	38.0	41.0	33.0	41.0
41	37.64009145991412	40.0	37.0	41.0	33.0	41.0
42	37.52248936302828	40.0	37.0	41.0	33.0	41.0
43	37.20954096177251	40.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	1.0
16	0.0
17	4.0
18	10.0
19	12.0
20	29.0
21	67.0
22	102.0
23	188.0
24	354.0
25	539.0
26	800.0
27	1066.0
28	1537.0
29	2026.0
30	2704.0
31	3320.0
32	4431.0
33	5787.0
34	8237.0
35	11360.0
36	17133.0
37	32845.0
38	66231.0
39	146048.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04586445693215	19.931569088648537	12.936263462289189	24.08630299213012
2	19.904013016963386	21.33824093848107	35.24552787919943	23.512218165356114
3	18.275580399759868	26.148087641429896	30.21162406957252	25.364707889237714
4	12.970052454950743	17.543704257741126	38.46433949080316	31.021903796504972
5	12.180439781782155	38.70578316652068	36.42158165290503	12.692195398792125
6	29.934095061886346	39.111907175404234	15.685637709828004	15.268360052881413
7	27.25623538133995	30.10074368588703	24.82966083068434	17.813360102088684
8	25.408994433017423	34.694078397023944	19.880721575419987	20.01620559453865
9	24.874275422936492	14.766117841572271	20.897671839991077	39.461934895500164
10	17.989522131790192	26.3203130894621	34.38538478445575	21.304779994291955
11	32.68773393956691	24.563285471061207	22.723917686077293	20.02506290329459
12	19.594007210505424	29.08576171215059	29.10675681438689	22.213474262957096
13	28.909599682449077	21.024954647298685	26.085758431665862	23.979687238586372
14	21.66924184717534	22.0793024377282	27.04858069828398	29.202875016812484
15	23.91014096242861	29.890792663523964	23.562737630112228	22.636328743935206
16	22.182309658075077	26.277666788044602	27.16733424530809	24.372689308572234
17	21.360548234607148	28.74951202789724	27.018728287291733	22.871211450203884
18	21.10926310471635	27.241145151607604	27.825727529499762	23.82386421417629
19	22.6471543435258	25.948962218657428	30.533767669510848	20.870115768305926
20	22.419488703650853	26.502051943195127	29.475483297412026	21.602976055741998
21	21.063992415519316	27.351697486820655	28.961759389567405	22.62255070809263
22	21.860494106609192	26.826819930912993	29.004733739457343	22.307952223020475
23	22.05076222062572	27.461921773561258	28.87974727145683	21.60756873435619
24	20.158578631578603	28.748855930952356	28.171818667926374	22.920746769542667
25	22.249231546453306	26.780237047826187	29.706101373538957	21.264430032181554
26	20.502045382225678	28.35519776402161	29.180895769158848	21.96186108459386
27	20.60242821479302	28.199374739611528	28.846286327267716	22.351910718327737
28	21.203084967834847	27.309051185403156	30.55148228702273	20.936381559739267
29	21.62921993353738	27.783081228082263	29.911787765760266	20.67591107262009
30	21.152237454606293	28.328625837753783	28.282371003139424	22.236765704500495
31	22.189198675996366	27.869686024807027	29.093634875489204	20.847480423707406
32	21.36546896169378	27.802436087956355	28.69308769063717	22.139007259712695
33	20.581761161029153	28.511676885376584	28.67570112159773	22.230860831996534
34	22.078318292310872	26.00669874980727	29.501399126734967	22.41358383114689
35	21.743708850419736	26.70905052930621	29.563728336499	21.98351228377505
36	22.026158585192547	27.357930407797053	28.077012659390547	22.538898347619845
37	21.066288754826413	25.717688045585618	30.134860727021024	23.081162472566945
38	20.94228643224323	26.643440834817756	30.590192006770923	21.8240807261681
39	20.025390951767033	26.841582112172897	30.60265784872373	22.530369087336343
40	20.51943195126512	25.728185596703767	31.638306876224032	22.11407557580708
41	20.48957954027287	25.069136215567212	30.87264174154373	23.568642502616186
42	18.8273579304078	26.660827403857194	30.533439621038404	23.978375044696605
43	19.961093451168345	24.750929197298195	31.957169991437933	23.33080736009553
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	377.0
1	318.0
2	259.0
3	428.0
4	597.0
5	597.0
6	667.0
7	737.0
8	632.5
9	528.0
10	721.0
11	914.0
12	914.0
13	1660.5
14	2407.0
15	3240.5
16	4074.0
17	3620.0
18	3166.0
19	3166.0
20	3458.0
21	3750.0
22	2741.0
23	1732.0
24	1742.5
25	1753.0
26	1753.0
27	2235.5
28	2718.0
29	4184.0
30	5650.0
31	7514.5
32	9379.0
33	9379.0
34	11320.0
35	13261.0
36	14980.5
37	16700.0
38	18846.0
39	20992.0
40	20992.0
41	24635.0
42	28278.0
43	29802.5
44	31327.0
45	30896.5
46	30466.0
47	30466.0
48	29774.0
49	29082.0
50	28445.5
51	27809.0
52	24115.5
53	20422.0
54	20422.0
55	19040.5
56	17659.0
57	13521.5
58	9384.0
59	7937.5
60	6491.0
61	6491.0
62	5698.0
63	4905.0
64	4158.5
65	3412.0
66	2844.0
67	2276.0
68	2276.0
69	1997.5
70	1719.0
71	1453.0
72	1187.0
73	905.0
74	623.0
75	623.0
76	498.0
77	373.0
78	294.5
79	216.0
80	161.5
81	107.0
82	107.0
83	77.5
84	48.0
85	39.0
86	30.0
87	21.0
88	12.0
89	12.0
90	10.0
91	8.0
92	6.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	304833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.72775585320487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.58427304164788	24.409758785958214
2	10.232045306470205	6.697437613381753
3	3.9091865884829353	3.83816712757477
4	2.114970179922819	2.768729107412911
5	1.354182328471909	2.215967431347656
6	0.9953390467598857	1.9545127988111521
7	0.736731318598707	1.6878093907155722
8	0.6094321655891345	1.5956277699592891
9	0.5482884779231193	1.6149826298333843
>10	4.216909737884027	28.408341616557266
>50	0.46609532401142684	10.659279015067266
>100	0.22753470656041697	11.91439247063146
>500	0.0020047110710168896	0.4576276190569919
>1k	0.0030070666065253344	1.777366623692317
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2081	0.6826688711524015	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1991	0.6531445086325955	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1346	0.44155324390731976	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	873	0.2863863164421175	No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATT	522	0.17124130261487439	No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAA	453	0.1486059580163565	No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACT	414	0.13581206759110726	No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAA	408	0.13384377675645354	No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCG	390	0.12793890425249232	No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC	377	0.1236742741107426	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.12203403174853115	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.11317672299258938	No Hit
GGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATC	343	0.1125206260477048	No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACAT	332	0.10891209285083964	No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAG	328	0.10759989896107049	No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTAT	325	0.10661575354374363	No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACC	306	0.10038283256734015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.280484724422881E-4	0.0
8	0.0	0.0	0.0	3.280484724422881E-4	0.0
9	0.0	0.0	0.0	3.280484724422881E-4	0.0
10	0.0	0.0	0.0	3.280484724422881E-4	0.0
11	0.0	0.0	0.0	3.280484724422881E-4	0.0
12	0.0	0.0	0.0	3.280484724422881E-4	0.0
13	0.0	0.0	0.0	3.280484724422881E-4	0.0
14	0.0	0.0	0.0	3.280484724422881E-4	0.0
15	0.0	0.0	0.0	3.280484724422881E-4	0.0
16	0.0	0.0	0.0	3.280484724422881E-4	0.0
17	0.0	0.0	0.0	6.560969448845762E-4	0.0
18	0.0	0.0	0.0	6.560969448845762E-4	0.0
19	0.0	0.0	0.0	6.560969448845762E-4	0.0
20	0.0	0.0	0.0	6.560969448845762E-4	0.0
21	0.0	0.0	0.0	9.841454173268641E-4	0.0
22	0.0	0.0	0.0	9.841454173268641E-4	0.0
23	0.0	0.0	0.0	0.0029524362519805928	0.0
24	0.0	0.0	0.0	0.0052487755590766095	0.0
25	0.0	0.0	0.0	0.011809745007922371	0.0
26	0.0	0.0	0.0	0.020995102236306438	0.0
27	0.0	0.0	0.0	0.0767633425514954	0.0
28	0.0	0.0	0.0	0.18042665984325845	0.0
29	0.0	0.0	0.0	0.29294728589096325	0.0
30	0.0	0.0	0.0	0.41465326916705214	0.0
31	0.0	0.0	0.0	0.71973834853838	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTAAG	20	0.0018398152	37.0	1
CTCTAAT	40	1.5949645E-6	32.375	1
GCTCTAG	30	3.59333E-4	30.833332	1
AACTTCC	25	0.0054900865	29.6	22
AGGGTTG	25	0.0054900865	29.6	5
ATTTGGG	25	0.0054900865	29.6	11
GCCGAGT	45	3.995565E-6	28.777777	12
CCGAGTT	45	3.995565E-6	28.777777	13
CCCCTTG	40	5.9260365E-5	27.75	9
AGTCCAA	40	5.9260365E-5	27.75	30
ACTGTTA	40	5.9260365E-5	27.75	24
GAGTTCC	40	5.9260365E-5	27.75	15
GGTATCA	460	0.0	27.347826	1
ATCTCGT	35	8.854387E-4	26.428572	37
GAATCTC	35	8.854387E-4	26.428572	35
CGAGTTC	45	1.3200984E-4	24.666666	14
ACCAGTA	45	1.3200984E-4	24.666666	12
CCCTTGT	45	1.3200984E-4	24.666666	10
CAGTATT	45	1.3200984E-4	24.666666	14
TAGTCCA	45	1.3200984E-4	24.666666	29
>>END_MODULE