FastQCFastQC Report
Fri 10 Feb 2017
ERR1630039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166290
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112620.5198749936527427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105140.48534591398196913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79630.3675869805058418No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG72770.33591993685056015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60370.2786792165407217No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC57440.26515378827396147No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG55460.2560137377728744No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT46260.21354481625267163No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC43780.20209667219070393No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC42690.19706502822798422No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG39980.18455516112801149No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA39960.18446283738557626No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG35330.16308989101182206No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA34870.16096644493581191No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC34730.16032017873876536No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG33170.15311892682881792No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC32980.15224185127568332No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31500.14540989433547677No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG31400.14494827562330068No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC31300.14448665691112456No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT30440.14051673598640993No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.13737772874361237No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT29400.13571590137977835No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT29390.13566973950856073No Hit
CCCCATCTCTCTTCCCTAATCCTCCAAGTCTTCAGCGAGACCC28840.13313083659159208No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA27590.12736060268939062No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA27570.1272682789469554No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA26840.12389846234806974No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC26740.12343684363589362No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC26550.122559768082759No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA26170.12080561697648975No Hit
CTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGA25870.11942076083996142No Hit
CTTTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATC25800.11909762774143812No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA25220.11642023921081665No Hit
GGCTTGAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGC24740.11420446939237128No Hit
CCTCTGATCCCTCGCCCCCACACCCCATCTCTCTTCCCTAATC24240.11189637583149072No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA24100.11125010963444415No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG24090.11120394776322653No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT23980.1106961671798328No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG23850.11009606285400386No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG23540.10866504484625789No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC23460.10829574987651698No Hit
TTCTTAACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCC23150.10686473186877103No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT23140.10681856999755343No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA23000.10617230380050686No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG22820.10534139011858985No Hit
GAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCC22660.10460280017910806No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG22380.10331026778501494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.029.1743981
CGTGCGA705.105365E-621.14285710
GTATCAA43400.018.073732
CGGTTAC650.001580544317.07692322
TTAACGG1302.5918598E-715.65384635
CCGTGTT1406.00141E-714.5357149
TAACGGC1406.00141E-714.53571436
CGCTAAG908.2781824E-414.38888835
ATTACGT1051.6566986E-414.09523817
GTACTAT2650.013.9622646
ACGCCGG800.00630099413.87514
CTATCGC800.00630099413.87530
ACATTCG800.00630099413.87528
ACGACCG800.00630099413.87530
TACGTTA950.001245696113.63157919
CGACCAA1501.3069093E-613.56666712
CTTAACG1102.4579334E-413.45454530
ACGACCA1805.1650204E-813.36111111
CTATACC2254.0745363E-1013.1555564
CGTCTAA850.00940824313.0588241
Produced by FastQC (version 0.11.5)